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Article Open Access

Diagnosis of pancreatic lesions collected by endoscopic ultrasound-guided fine-needle aspiration using next-generation sequencing

  • Authors:
    • Eri Kameta
    • Kazuya Sugimori
    • Takashi Kaneko
    • Tomohiro Ishii
    • Haruo Miwa
    • Takeshi Sato
    • Yasuaki Ishii
    • Soichiro Sue
    • Tomohiko Sasaki
    • Yuki Yamashita
    • Wataru Shibata
    • Naomichi Matsumoto
    • Shin Maeda
  • View Affiliations / Copyright

    Affiliations: Department of Gastroenterology, Yokohama City University Graduate School of Medicine, Yokohama 236‑0004, Japan, Gastroenterological Centre, Yokohama City University Medical Centre, Yokohama 232‑0024, Japan, Department of Human Genetics, Yokohama City University Graduate School of Medicine, Yokohama 236‑0004, Japan
    Copyright: © Kameta et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 3875-3881
    |
    Published online on: September 21, 2016
       https://doi.org/10.3892/ol.2016.5168
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Abstract

Endoscopic ultrasound-guided fine‑needle aspiration (EUF‑FNA) has improved the diagnosis of pancreatic lesions. Next-generation sequencing (NGS) facilitates the production of millions of sequences concurrently. Therefore, in the current study, to improve the detectability of oncogenic mutations in pancreatic lesions, an NGS system was used to diagnose EUS‑FNA samples. A total of 38 patients with clinically diagnosed EUS‑FNA specimens were analyzed; 27 patients had pancreatic ductal adenocarcinoma (PDAC) and 11 had non‑PDAC lesions. DNA samples were isolated and sequenced by NGS using an Ion Personal Genome Machine system. The Cancer Hotspot Panel v2, which includes 50 cancer-related genes and 2,790 COSMIC mutations, was used. A >2% mutation frequency was defined as positive. KRAS mutations were detected in 26 of 27 PDAC aspirates (96%) and 0 of 11 non‑PDAC lesions (0%). The G12, G13, and Q61 KRAS mutations were found in 25, 0, and 1 of the 27 PDAC samples, respectively. Mutations were confirmed by TaqMan® polymerase chain reaction analysis. TP53 mutations were detected in 12 of 27 PDAC aspirates (44%). SMAD4 was observed in 3 PDAC lesions and cyclin-dependent kinase inhibitor 2A in 4 PDAC lesions. Therefore, the current study was successfully able to develop an NGS assay with high clinical sensitivity for EUS-FNA samples.
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Copy and paste a formatted citation
Spandidos Publications style
Kameta E, Sugimori K, Kaneko T, Ishii T, Miwa H, Sato T, Ishii Y, Sue S, Sasaki T, Yamashita Y, Yamashita Y, et al: Diagnosis of pancreatic lesions collected by endoscopic ultrasound-guided fine-needle aspiration using next-generation sequencing. Oncol Lett 12: 3875-3881, 2016.
APA
Kameta, E., Sugimori, K., Kaneko, T., Ishii, T., Miwa, H., Sato, T. ... Maeda, S. (2016). Diagnosis of pancreatic lesions collected by endoscopic ultrasound-guided fine-needle aspiration using next-generation sequencing. Oncology Letters, 12, 3875-3881. https://doi.org/10.3892/ol.2016.5168
MLA
Kameta, E., Sugimori, K., Kaneko, T., Ishii, T., Miwa, H., Sato, T., Ishii, Y., Sue, S., Sasaki, T., Yamashita, Y., Shibata, W., Matsumoto, N., Maeda, S."Diagnosis of pancreatic lesions collected by endoscopic ultrasound-guided fine-needle aspiration using next-generation sequencing". Oncology Letters 12.5 (2016): 3875-3881.
Chicago
Kameta, E., Sugimori, K., Kaneko, T., Ishii, T., Miwa, H., Sato, T., Ishii, Y., Sue, S., Sasaki, T., Yamashita, Y., Shibata, W., Matsumoto, N., Maeda, S."Diagnosis of pancreatic lesions collected by endoscopic ultrasound-guided fine-needle aspiration using next-generation sequencing". Oncology Letters 12, no. 5 (2016): 3875-3881. https://doi.org/10.3892/ol.2016.5168
Copy and paste a formatted citation
x
Spandidos Publications style
Kameta E, Sugimori K, Kaneko T, Ishii T, Miwa H, Sato T, Ishii Y, Sue S, Sasaki T, Yamashita Y, Yamashita Y, et al: Diagnosis of pancreatic lesions collected by endoscopic ultrasound-guided fine-needle aspiration using next-generation sequencing. Oncol Lett 12: 3875-3881, 2016.
APA
Kameta, E., Sugimori, K., Kaneko, T., Ishii, T., Miwa, H., Sato, T. ... Maeda, S. (2016). Diagnosis of pancreatic lesions collected by endoscopic ultrasound-guided fine-needle aspiration using next-generation sequencing. Oncology Letters, 12, 3875-3881. https://doi.org/10.3892/ol.2016.5168
MLA
Kameta, E., Sugimori, K., Kaneko, T., Ishii, T., Miwa, H., Sato, T., Ishii, Y., Sue, S., Sasaki, T., Yamashita, Y., Shibata, W., Matsumoto, N., Maeda, S."Diagnosis of pancreatic lesions collected by endoscopic ultrasound-guided fine-needle aspiration using next-generation sequencing". Oncology Letters 12.5 (2016): 3875-3881.
Chicago
Kameta, E., Sugimori, K., Kaneko, T., Ishii, T., Miwa, H., Sato, T., Ishii, Y., Sue, S., Sasaki, T., Yamashita, Y., Shibata, W., Matsumoto, N., Maeda, S."Diagnosis of pancreatic lesions collected by endoscopic ultrasound-guided fine-needle aspiration using next-generation sequencing". Oncology Letters 12, no. 5 (2016): 3875-3881. https://doi.org/10.3892/ol.2016.5168
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