Open Access

Osthole induces lung cancer cell apoptosis through inhibition of inhibitor of apoptosis family proteins

  • Authors:
    • Xiao‑Man Xu
    • Man‑Li Zhang
    • Yi Zhang
    • Li Zhao
  • View Affiliations

  • Published online on: September 22, 2016     https://doi.org/10.3892/ol.2016.5170
  • Pages: 3779-3784
  • Copyright: © Xu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

In the present study, we investigated the effects and mechanisms of Osthole on the apoptosis of non‑small cell lung cancer (NSCLC) cells and its synergistic effect with Embelin. Our results revealed that treatment with both Osthole and Embelin inhibited cell proliferation. Notably, combination treatment of Osthole and Embelin inhibited cell proliferation more significantly compared with monotherapy. In addition, morphological analysis and Annexin V/propidium iodide analysis revealed that the combination of Osthole and Embelin enhanced their effect on cell apoptosis. We further examined the effect of Osthole on the expression of inhibitor of apoptosis protein (IAP) family proteins. That treatment of A549 lung cancer cells with various concentrations of Osthole was observed to decrease the protein expression of X‑chromosome‑encoded IAP, c‑IAP1, c‑IAP2 and Survivin, and increase Smac expression in a dose‑dependent manner. Furthermore, it was noted that Osthole or Embelin alone increased the expression of BAX, caspase‑3, caspase‑9, cleaved caspase‑3 and cleaved caspase‑9, and decreased Bcl‑2 levels following treatment. Osthole and Embelin combination treatment had a synergistic effect on the regulation of these proteins. In conclusion, our study demonstrated that Osthole inhibited proliferation and induced the apoptosis of lung cancer cells via IAP family proteins in a dose‑dependent manner. Osthole enhances the antitumor effect of Embelin, indicating that combination of Osthole and Embelin has potential clinical significance in the treatment of NSCLC.
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November-2016
Volume 12 Issue 5

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Xu XM, Zhang ML, Zhang Y and Zhao L: Osthole induces lung cancer cell apoptosis through inhibition of inhibitor of apoptosis family proteins. Oncol Lett 12: 3779-3784, 2016
APA
Xu, X., Zhang, M., Zhang, Y., & Zhao, L. (2016). Osthole induces lung cancer cell apoptosis through inhibition of inhibitor of apoptosis family proteins. Oncology Letters, 12, 3779-3784. https://doi.org/10.3892/ol.2016.5170
MLA
Xu, X., Zhang, M., Zhang, Y., Zhao, L."Osthole induces lung cancer cell apoptosis through inhibition of inhibitor of apoptosis family proteins". Oncology Letters 12.5 (2016): 3779-3784.
Chicago
Xu, X., Zhang, M., Zhang, Y., Zhao, L."Osthole induces lung cancer cell apoptosis through inhibition of inhibitor of apoptosis family proteins". Oncology Letters 12, no. 5 (2016): 3779-3784. https://doi.org/10.3892/ol.2016.5170