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Inhibition of cell proliferation and tumor growth of colorectal cancer by inhibitors of Wnt and Notch signaling pathways

  • Authors:
    • Yuliang Xiao
    • Xiaojing Yang
    • Yinglei Miao
    • Xikun He
    • Ming Wang
    • Weihong Sha
  • View Affiliations / Copyright

    Affiliations: Department of Graduate School, Southern Medical University, Guangzhou, Guangdong 510515, P.R. China, Foreign Language Department, Kunming Medical University, Kunming, Yunan 650032, P.R. China, Department of Gastroenterology, The First Affiliated Hospital of Kunming Medical University, Kunming, Yunan 650032, P.R. China, Department of Gastroenterology, The Second People's Hospital of Yunnan, Kunming, Yunan 650021, P.R. China, Department of Gastroenterology, Guangdong General Hospital, Guangdong Academy of Medical Sciences, Guangzhou, Guangdong 510080, P.R. China
    Copyright: © Xiao et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 3695-3700
    |
    Published online on: September 22, 2016
       https://doi.org/10.3892/ol.2016.5175
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Abstract

Understanding the role and mechanism of signaling pathways including Notch and Wnt in colorectal carcinogenesis is critical to the development of novel therapeutics. In the present study, we analyzed the cell proliferation, migration, G2/M percentage and the expression of molecules of signaling pathways in HCT‑116 cells through the inhibition of Wnt and Notch pathways, and also investigated the effect of inhibitors of Wnt and Notch pathways on tumor growth in a transplantation tumor model. We observed that rDDK‑1 (an inhibitor of the Wnt signaling pathway) and LY374973 (an inhibitor of the Notch signaling pathway) synergistically inhibited the proliferation, migration and G2/M percentage of HCT‑116 cell lines, and could further synergistically inhibit the tumor volume and weight in the transplantation tumor model. In the cell line and the transplantation tumor model, rDDK‑1 and LY374973 further synergistically inhibited the expression level of all detected Wnt and Notch pathway genes. Our results may pave the way for using inhibitors of Wnt and Notch signaling pathways together to treat colorectal cancer.
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Copy and paste a formatted citation
Spandidos Publications style
Xiao Y, Yang X, Miao Y, He X, Wang M and Sha W: Inhibition of cell proliferation and tumor growth of colorectal cancer by inhibitors of Wnt and Notch signaling pathways. Oncol Lett 12: 3695-3700, 2016.
APA
Xiao, Y., Yang, X., Miao, Y., He, X., Wang, M., & Sha, W. (2016). Inhibition of cell proliferation and tumor growth of colorectal cancer by inhibitors of Wnt and Notch signaling pathways. Oncology Letters, 12, 3695-3700. https://doi.org/10.3892/ol.2016.5175
MLA
Xiao, Y., Yang, X., Miao, Y., He, X., Wang, M., Sha, W."Inhibition of cell proliferation and tumor growth of colorectal cancer by inhibitors of Wnt and Notch signaling pathways". Oncology Letters 12.5 (2016): 3695-3700.
Chicago
Xiao, Y., Yang, X., Miao, Y., He, X., Wang, M., Sha, W."Inhibition of cell proliferation and tumor growth of colorectal cancer by inhibitors of Wnt and Notch signaling pathways". Oncology Letters 12, no. 5 (2016): 3695-3700. https://doi.org/10.3892/ol.2016.5175
Copy and paste a formatted citation
x
Spandidos Publications style
Xiao Y, Yang X, Miao Y, He X, Wang M and Sha W: Inhibition of cell proliferation and tumor growth of colorectal cancer by inhibitors of Wnt and Notch signaling pathways. Oncol Lett 12: 3695-3700, 2016.
APA
Xiao, Y., Yang, X., Miao, Y., He, X., Wang, M., & Sha, W. (2016). Inhibition of cell proliferation and tumor growth of colorectal cancer by inhibitors of Wnt and Notch signaling pathways. Oncology Letters, 12, 3695-3700. https://doi.org/10.3892/ol.2016.5175
MLA
Xiao, Y., Yang, X., Miao, Y., He, X., Wang, M., Sha, W."Inhibition of cell proliferation and tumor growth of colorectal cancer by inhibitors of Wnt and Notch signaling pathways". Oncology Letters 12.5 (2016): 3695-3700.
Chicago
Xiao, Y., Yang, X., Miao, Y., He, X., Wang, M., Sha, W."Inhibition of cell proliferation and tumor growth of colorectal cancer by inhibitors of Wnt and Notch signaling pathways". Oncology Letters 12, no. 5 (2016): 3695-3700. https://doi.org/10.3892/ol.2016.5175
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