Corosolic acid inhibits the proliferation of osteosarcoma cells by inducing apoptosis

  • Authors:
    • Yong Jia
    • Hua Yuan
    • Shouqin Shan
    • Gang Xu
    • Jie Yu
    • Chenguang Zhao
    • Xiang Mou
  • View Affiliations

  • Published online on: September 26, 2016     https://doi.org/10.3892/ol.2016.5185
  • Pages: 4187-4194
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Abstract

Corosolic acid (CRA), a pentacyclic triterpene isolated from medicinal herbs, has been reported to exhibit anticancer properties in several cancers. However, the anticancer activity of CRA in osteosarcoma cells is still unclear. In the present study, the inhibitory effect of CRA in osteosarcoma MG‑63 cells was investigated, and the results revealed that CRA significantly inhibited the viability of MG‑63 cells in a dose‑ and time‑dependent manner. A typical apoptotic hallmark such as DNA ladder was detected by agarose gel electrophoresis following treatment with CRA. Further experiments demonstrated that CRA induced apoptosis of MG‑63 cells by flow cytometry using propidium iodide and annexin V staining. In addition, it was observed that the apoptosis of MG‑63 cells induced by CRA was closely associated with activation of caspase‑3 and caspase‑9, loss of mitochondrial membrane potential, and release of cytochrome c from mitochondria, suggesting that CRA may trigger the activation of the mitochondria‑mediated apoptosis pathway. In addition, the inhibition of caspase activity attenuated the CRA‑induced apoptosis of MG‑63 cells, which further confirmed the role of the mitochondrial pathway in CRA‑induced apoptosis. These results indicated that CRA could induce the apoptosis of osteosarcoma cells through activating the mitochondrial pathway, which provides an evidence that CRA may be a useful chemotherapeutic agent for osteosarcoma.
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November-2016
Volume 12 Issue 5

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Jia Y, Yuan H, Shan S, Xu G, Yu J, Zhao C and Mou X: Corosolic acid inhibits the proliferation of osteosarcoma cells by inducing apoptosis. Oncol Lett 12: 4187-4194, 2016
APA
Jia, Y., Yuan, H., Shan, S., Xu, G., Yu, J., Zhao, C., & Mou, X. (2016). Corosolic acid inhibits the proliferation of osteosarcoma cells by inducing apoptosis. Oncology Letters, 12, 4187-4194. https://doi.org/10.3892/ol.2016.5185
MLA
Jia, Y., Yuan, H., Shan, S., Xu, G., Yu, J., Zhao, C., Mou, X."Corosolic acid inhibits the proliferation of osteosarcoma cells by inducing apoptosis". Oncology Letters 12.5 (2016): 4187-4194.
Chicago
Jia, Y., Yuan, H., Shan, S., Xu, G., Yu, J., Zhao, C., Mou, X."Corosolic acid inhibits the proliferation of osteosarcoma cells by inducing apoptosis". Oncology Letters 12, no. 5 (2016): 4187-4194. https://doi.org/10.3892/ol.2016.5185