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Article Open Access

PBX3 promotes gastric cancer invasion and metastasis by inducing epithelial-mesenchymal transition

  • Authors:
    • Shuanhu Wang
    • Chenglong Li
    • Wenbin Wang
    • Chungen Xing
  • View Affiliations / Copyright

    Affiliations: Department of General Surgery, The Second Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215000, P.R. China, Department of Vascular Surgery, The Second Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215000, P.R. China, Department of General Surgery, The Fourth Affiliated Hospital of Anhui Medical University, Hefei, Anhui 230000, P.R. China
    Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 3485-3491
    |
    Published online on: October 21, 2016
       https://doi.org/10.3892/ol.2016.5305
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Abstract

The overexpression of pre-leukemia transcription factor 3 (PBX3) in tumors plays an important role in invasion, metastasis and proliferation in a variety of human cancer types. Tumor metastasis and angiogenesis significantly contribute to the progression of cancer and create challenges for cancer therapy. In the present study, reverse transcription-polymerase chain reaction demonstrated that PBX3 was upregulated in gastric cancer (GC) tissues and Transwell assay revealed that the overexpression of PBX3 promoted GC invasion and metastasis in vitro. In addition, a nude mouse xenograft model was established, which demonstrated that PBX3 promoted peritoneal metastases in vivo. Furthermore, the overexpression of PBX3 in GC promoted the tubular formation of human umbilical vein endothelial cells. Western blot analysis revealed that overexpressed PBX3 induced epithelial‑mesenchymal transition (EMT) in GC, as measured by increases in the EMT protein markers N‑cadherin and vimentin, while E‑cadherin expression was reduced in PBX3-overexpressing GC cells. Contrasting results were observed in PBX3‑knockdown GC cells. Additionally, the overexpression of PBX3 increased the levels of phosphorylated AKT (Ser473), which is involved in the progression of a variety of human cancers. Gelatin zymography assay demonstrated that the overexpression of PBX3 also elevated matrix metalloproteinase‑9 activity in GC, which was closely associated with tumor metastasis and angiogenesis. Based on these findings, it may be concluded that PBX3 enhances invasion and metastasis in GC by promoting EMT, possibly via the AKT signaling pathway.
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Copy and paste a formatted citation
Spandidos Publications style
Wang S, Li C, Wang W and Xing C: PBX3 promotes gastric cancer invasion and metastasis by inducing epithelial-mesenchymal transition. Oncol Lett 12: 3485-3491, 2016.
APA
Wang, S., Li, C., Wang, W., & Xing, C. (2016). PBX3 promotes gastric cancer invasion and metastasis by inducing epithelial-mesenchymal transition. Oncology Letters, 12, 3485-3491. https://doi.org/10.3892/ol.2016.5305
MLA
Wang, S., Li, C., Wang, W., Xing, C."PBX3 promotes gastric cancer invasion and metastasis by inducing epithelial-mesenchymal transition". Oncology Letters 12.5 (2016): 3485-3491.
Chicago
Wang, S., Li, C., Wang, W., Xing, C."PBX3 promotes gastric cancer invasion and metastasis by inducing epithelial-mesenchymal transition". Oncology Letters 12, no. 5 (2016): 3485-3491. https://doi.org/10.3892/ol.2016.5305
Copy and paste a formatted citation
x
Spandidos Publications style
Wang S, Li C, Wang W and Xing C: PBX3 promotes gastric cancer invasion and metastasis by inducing epithelial-mesenchymal transition. Oncol Lett 12: 3485-3491, 2016.
APA
Wang, S., Li, C., Wang, W., & Xing, C. (2016). PBX3 promotes gastric cancer invasion and metastasis by inducing epithelial-mesenchymal transition. Oncology Letters, 12, 3485-3491. https://doi.org/10.3892/ol.2016.5305
MLA
Wang, S., Li, C., Wang, W., Xing, C."PBX3 promotes gastric cancer invasion and metastasis by inducing epithelial-mesenchymal transition". Oncology Letters 12.5 (2016): 3485-3491.
Chicago
Wang, S., Li, C., Wang, W., Xing, C."PBX3 promotes gastric cancer invasion and metastasis by inducing epithelial-mesenchymal transition". Oncology Letters 12, no. 5 (2016): 3485-3491. https://doi.org/10.3892/ol.2016.5305
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