Identification of proteins associated with pediatric bilateral Wilms tumor

Yan,Zechen; Meng,Qingjun; Yang,Jinjian; Zhang,Junjie; Zhao,Wei; Guo,Fei; Song,Dongjian; Zhan,Yuxiao; Fan,Dandan; Zhou,Ruiyi; Zuo,Shuqiang; Wang,Zehua; Yu,Jiekai; Zheng,Shu; Wang,Jiaxiang

doi:10.3892/ol.2016.5306

December-2016 Volume 12 Issue 6

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December-2016 Volume 12 Issue 6

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Article Open Access

Identification of proteins associated with pediatric bilateral Wilms tumor

Authors:
- Zechen Yan
- Qingjun Meng
- Jinjian Yang
- Junjie Zhang
- Wei Zhao
- Fei Guo
- Dongjian Song
- Yuxiao Zhan
- Dandan Fan
- Ruiyi Zhou
- Shuqiang Zuo
- Zehua Wang
- Jiekai Yu
- Shu Zheng
- Jiaxiang Wang
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Affiliations: Department of Surgery, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450052, P.R. China, Henan Academy of Medical and Parmaceutical Science, Zhengzhou University, Zhengzhou, Henan 450052, P.R. China, Institute of Cancer, The Second Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, Zhejiang 310000, P.R. China

Copyright: © Yan et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
Pages: 5075-5079
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Published online on: October 21, 2016

https://doi.org/10.3892/ol.2016.5306
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Abstract

Wilms tumor (WT) is the most common cancer that primarily develops in abdominal solid organ of children. It has no incipient symptom, and the most frequent symptoms are a painless, palpable abdominal mass. Proteomics technology was used to select the differentially expressed proteins of bilateral Wilms tumor (BWT). Ten serum samples of children with BWT were chosen, 20 serum samples of children with unilateral WT (UWT) and 20 serum samples of healthy children were selected, and proteomics technology was used to detect and collect data. Using bioinformatics, the data were analyzed and 10 difference peaks were obtained (P<0.01). Non‑linear support vector machine was used to classify and to select the composite pattern with the highest Youden's index, and one differentially expressed protein with m/z of 5,648 kDa was obtained. A significantly high expression in children with BWT was obtained, and the expression intensity was also significantly (3,889.36±1,796.83) higher for children with BWT compared to those with UWT (2,886.81±1,404.65) and healthy children (432.21±730.42). Matrix‑assisted laser desorption ionization/time‑of‑flight ionization/time‑of‑flight mass spectrometry was used for identification of the peak, and the peak was further identified as apolipoprotein C‑Ⅲ (APO C‑Ⅲ) by western blot analysis. In conclusion, to the best of our knowledge, a differentially expressed protein of APO C‑Ⅲ of BWT was obtained through proteomics technology for the first time, and it is expected to be a new marker for the early diagnosis and prognosis of BWT.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Identification of proteins associated with pediatric bilateral Wilms tumor

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