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Article

Naphthazarin suppresses cell proliferation and induces apoptosis in human colorectal cancer cells via the B-cell lymphoma 2/B-cell associated X protein signaling pathway

  • Authors:
    • Ai‑Dong Chen
    • Hui Li
    • Yong‑Chun Li
    • Hai Zeng
  • View Affiliations / Copyright

    Affiliations: Department of Gastroenterology, Hongqi Hospital Affiliated to Mudanjiang Medical University, Mudanjiang, Heilongjiang 157011, P.R. China, Department of Medical Oncology, Hongqi Hospital Affiliated to Mudanjiang Medical University, Mudanjiang, Heilongjiang 157011, P.R. China, Department of Second General Surgery, Hongqi Hospital Affiliated to Mudanjiang Medical University, Mudanjiang, Heilongjiang 157011, P.R. China
  • Pages: 5211-5216
    |
    Published online on: October 26, 2016
       https://doi.org/10.3892/ol.2016.5319
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Abstract

Colorectal cancer is the most common gastrointestinal cancer in the USA. Naphthazarin, one of the naturally available 1,4‑naphthoquinone derivatives, is a natural bioactive molecule that exhibits an antitumor effect. To the best of our knowledge, this is the first study to investigate the anticancer effect of naphthazarin on cell proliferation and apoptosis in human SW480 colorectal cancer cells. In the present study, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide and lactate dehydrogenase assays were performed to assess the effect of napthazarin on cell proliferation and cytotoxicity of SW430 cells, respectively. In addition, an Annexin V‑fluorescein isothiocyanate/propidium iodide apoptosis assay and 4',6-diamidino-2-phenylindole staining were used to analyze cell and nuclei apoptosis of SW480 cells, respectively, following treatment with naphthazarin. Poly (ADP-ribose) polymerase (PARP), B‑cell lymphoma 2 (Bcl‑2) and B‑cell associated X protein (Bax) protein expression was analyzed by western blot. Furthermore, caspase‑3 activation was analyzed using a commercial kit. The results revealed that naphthazarin exhibited cell growth inhibition, an increase in cytotoxicity and apoptosis induction in SW480 cells, which was associated with activation of the Bax/Bcl‑2 signaling pathway and cleaved caspase‑3 activation. However, no significant differences in PARP expression were identified following treatment with naphthazarin in SW480 cells. Taken together, these results suggest that naphthazarin decreased cell viability and induced apoptosis of SW480 cells, indicating that naphthazarin may present a potential therapeutic agent for human colorectal cancer treatment.
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Copy and paste a formatted citation
Spandidos Publications style
Chen AD, Li H, Li YC and Zeng H: Naphthazarin suppresses cell proliferation and induces apoptosis in human colorectal cancer cells via the B-cell lymphoma 2/B-cell associated X protein signaling pathway. Oncol Lett 12: 5211-5216, 2016.
APA
Chen, A., Li, H., Li, Y., & Zeng, H. (2016). Naphthazarin suppresses cell proliferation and induces apoptosis in human colorectal cancer cells via the B-cell lymphoma 2/B-cell associated X protein signaling pathway. Oncology Letters, 12, 5211-5216. https://doi.org/10.3892/ol.2016.5319
MLA
Chen, A., Li, H., Li, Y., Zeng, H."Naphthazarin suppresses cell proliferation and induces apoptosis in human colorectal cancer cells via the B-cell lymphoma 2/B-cell associated X protein signaling pathway". Oncology Letters 12.6 (2016): 5211-5216.
Chicago
Chen, A., Li, H., Li, Y., Zeng, H."Naphthazarin suppresses cell proliferation and induces apoptosis in human colorectal cancer cells via the B-cell lymphoma 2/B-cell associated X protein signaling pathway". Oncology Letters 12, no. 6 (2016): 5211-5216. https://doi.org/10.3892/ol.2016.5319
Copy and paste a formatted citation
x
Spandidos Publications style
Chen AD, Li H, Li YC and Zeng H: Naphthazarin suppresses cell proliferation and induces apoptosis in human colorectal cancer cells via the B-cell lymphoma 2/B-cell associated X protein signaling pathway. Oncol Lett 12: 5211-5216, 2016.
APA
Chen, A., Li, H., Li, Y., & Zeng, H. (2016). Naphthazarin suppresses cell proliferation and induces apoptosis in human colorectal cancer cells via the B-cell lymphoma 2/B-cell associated X protein signaling pathway. Oncology Letters, 12, 5211-5216. https://doi.org/10.3892/ol.2016.5319
MLA
Chen, A., Li, H., Li, Y., Zeng, H."Naphthazarin suppresses cell proliferation and induces apoptosis in human colorectal cancer cells via the B-cell lymphoma 2/B-cell associated X protein signaling pathway". Oncology Letters 12.6 (2016): 5211-5216.
Chicago
Chen, A., Li, H., Li, Y., Zeng, H."Naphthazarin suppresses cell proliferation and induces apoptosis in human colorectal cancer cells via the B-cell lymphoma 2/B-cell associated X protein signaling pathway". Oncology Letters 12, no. 6 (2016): 5211-5216. https://doi.org/10.3892/ol.2016.5319
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