Naphthazarin suppresses cell proliferation and induces apoptosis in human colorectal cancer cells via the B-cell lymphoma 2/B-cell associated X protein signaling pathway

  • Authors:
    • Ai‑Dong Chen
    • Hui Li
    • Yong‑Chun Li
    • Hai Zeng
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  • Published online on: October 26, 2016     https://doi.org/10.3892/ol.2016.5319
  • Pages: 5211-5216
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Abstract

Colorectal cancer is the most common gastrointestinal cancer in the USA. Naphthazarin, one of the naturally available 1,4‑naphthoquinone derivatives, is a natural bioactive molecule that exhibits an antitumor effect. To the best of our knowledge, this is the first study to investigate the anticancer effect of naphthazarin on cell proliferation and apoptosis in human SW480 colorectal cancer cells. In the present study, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide and lactate dehydrogenase assays were performed to assess the effect of napthazarin on cell proliferation and cytotoxicity of SW430 cells, respectively. In addition, an Annexin V‑fluorescein isothiocyanate/propidium iodide apoptosis assay and 4',6-diamidino-2-phenylindole staining were used to analyze cell and nuclei apoptosis of SW480 cells, respectively, following treatment with naphthazarin. Poly (ADP-ribose) polymerase (PARP), B‑cell lymphoma 2 (Bcl‑2) and B‑cell associated X protein (Bax) protein expression was analyzed by western blot. Furthermore, caspase‑3 activation was analyzed using a commercial kit. The results revealed that naphthazarin exhibited cell growth inhibition, an increase in cytotoxicity and apoptosis induction in SW480 cells, which was associated with activation of the Bax/Bcl‑2 signaling pathway and cleaved caspase‑3 activation. However, no significant differences in PARP expression were identified following treatment with naphthazarin in SW480 cells. Taken together, these results suggest that naphthazarin decreased cell viability and induced apoptosis of SW480 cells, indicating that naphthazarin may present a potential therapeutic agent for human colorectal cancer treatment.
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December-2016
Volume 12 Issue 6

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Chen AD, Li H, Li YC and Zeng H: Naphthazarin suppresses cell proliferation and induces apoptosis in human colorectal cancer cells via the B-cell lymphoma 2/B-cell associated X protein signaling pathway. Oncol Lett 12: 5211-5216, 2016
APA
Chen, A., Li, H., Li, Y., & Zeng, H. (2016). Naphthazarin suppresses cell proliferation and induces apoptosis in human colorectal cancer cells via the B-cell lymphoma 2/B-cell associated X protein signaling pathway. Oncology Letters, 12, 5211-5216. https://doi.org/10.3892/ol.2016.5319
MLA
Chen, A., Li, H., Li, Y., Zeng, H."Naphthazarin suppresses cell proliferation and induces apoptosis in human colorectal cancer cells via the B-cell lymphoma 2/B-cell associated X protein signaling pathway". Oncology Letters 12.6 (2016): 5211-5216.
Chicago
Chen, A., Li, H., Li, Y., Zeng, H."Naphthazarin suppresses cell proliferation and induces apoptosis in human colorectal cancer cells via the B-cell lymphoma 2/B-cell associated X protein signaling pathway". Oncology Letters 12, no. 6 (2016): 5211-5216. https://doi.org/10.3892/ol.2016.5319