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Article Open Access

Role of AXL expression in non-small cell lung cancer

  • Authors:
    • Xiaohan Qu
    • Jinlu Liu
    • Xinwen Zhong
    • Xi Li
    • Qigang Zhang
  • View Affiliations / Copyright

    Affiliations: Department of Thoracic Surgery, The First Hospital of China Medical University, Shenyang, Liaoning 110001, P.R. China, Department of Ophthalmology, The Fourth Affiliated Hospital of China Medical University, Shenyang, Liaoning 110034, P.R. China
    Copyright: © Qu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 5085-5091
    |
    Published online on: November 7, 2016
       https://doi.org/10.3892/ol.2016.5356
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Abstract

The present study aimed to investigate the expression profile of AXL in non-small cell lung cancer (NSCLC) and its clinical significance. The current study included 257 NSCLC patients, tyrosine‑protein kinase receptor UFO (AXL) expression in paired lung cancer and adjacent normal lung tissues of NSCLC patients were compared by immunohistochemistry, western blot analysis and quantitative polymerase chain reaction (qPCR). These methods were used to detect the expression of the AXL gene and protein in fresh tissues from 35 patients. Small interfering RNA (siRNA) was transfected into the H1299 lung cancer cell line to knock down AXL expression; the effects of AXL‑siRNA on cell proliferation and migration were examined by MTT and Transwell migration assay, respectively. It was found that AXL staining density in lung cancer tissues was significantly increased compared with adjacent normal lung tissues (55.25 vs. 26.85%; P<0.01); and the expression level of AXL in NSCLC patients was significantly associated with the degree of tumor differentiation (P<0.01) and the clinical stage of disease (P<0.01). Western blotting and qPCR showed that AXL expression was significantly higher in cancer tissues compared with that in adjacent lung tissue (P<0.05). Additionally, the current study also showed that AXL‑siRNA inhibited H1299 cell proliferation and migration in vitro. The present study demonstrates the association between increased expression of AXL in NSCLC and the low differentiation phenotype, and its effects on cell proliferation and migration, suggesting its potential clinical values for the prognosis of NSCLC.
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Copy and paste a formatted citation
Spandidos Publications style
Qu X, Liu J, Zhong X, Li X and Zhang Q: Role of AXL expression in non-small cell lung cancer. Oncol Lett 12: 5085-5091, 2016.
APA
Qu, X., Liu, J., Zhong, X., Li, X., & Zhang, Q. (2016). Role of AXL expression in non-small cell lung cancer. Oncology Letters, 12, 5085-5091. https://doi.org/10.3892/ol.2016.5356
MLA
Qu, X., Liu, J., Zhong, X., Li, X., Zhang, Q."Role of AXL expression in non-small cell lung cancer". Oncology Letters 12.6 (2016): 5085-5091.
Chicago
Qu, X., Liu, J., Zhong, X., Li, X., Zhang, Q."Role of AXL expression in non-small cell lung cancer". Oncology Letters 12, no. 6 (2016): 5085-5091. https://doi.org/10.3892/ol.2016.5356
Copy and paste a formatted citation
x
Spandidos Publications style
Qu X, Liu J, Zhong X, Li X and Zhang Q: Role of AXL expression in non-small cell lung cancer. Oncol Lett 12: 5085-5091, 2016.
APA
Qu, X., Liu, J., Zhong, X., Li, X., & Zhang, Q. (2016). Role of AXL expression in non-small cell lung cancer. Oncology Letters, 12, 5085-5091. https://doi.org/10.3892/ol.2016.5356
MLA
Qu, X., Liu, J., Zhong, X., Li, X., Zhang, Q."Role of AXL expression in non-small cell lung cancer". Oncology Letters 12.6 (2016): 5085-5091.
Chicago
Qu, X., Liu, J., Zhong, X., Li, X., Zhang, Q."Role of AXL expression in non-small cell lung cancer". Oncology Letters 12, no. 6 (2016): 5085-5091. https://doi.org/10.3892/ol.2016.5356
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