Open Access

DHA blocks TPA-induced cell invasion by inhibiting MMP-9 expression via suppression of the PPAR‑γ/NF-κB pathway in MCF-7 cells

  • Authors:
    • Jin‑Ki Hwang
    • Hong‑Nu Yu
    • Eun‑Mi Noh
    • Jeong‑Mi Kim
    • On‑Yu Hong
    • Hyun Jo Youn
    • Sung Hoo Jung
    • Kang‑Beom Kwon
    • Jong‑Suk Kim
    • Young‑Rae Lee
  • View Affiliations

  • Published online on: November 11, 2016     https://doi.org/10.3892/ol.2016.5382
  • Pages: 243-249
  • Copyright: © Hwang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Docosahexaenoic acid (DHA) is an omega-3 fatty acid that is considered to have applications in cancer prevention and treatment. The beneficial effects of DHA against cancer metastasis are well established; however, the mechanisms underlying these effects in breast cancer are not clear. Cell invasion is critical for neoplastic metastasis, and involves the degradation of the extracellular matrix by matrix metalloproteinase (MMP)-9. The present study investigated the inhibitory effect of DHA on MMP-9 expression and cell invasion induced by 12-O-tetradecanoylphorbol-13-acetate (TPA) in the MCF-7 breast cancer cell line. DHA inhibited the TPA‑induced activation of mitogen‑activated protein kinase (MAPK) and the transcription of nuclear factor (NF)‑κB, but did not inhibit the transcription of activator protein‑1. DHA increased the activity of peroxisome proliferator‑activated receptor (PPAR)‑γ, an effect that was reversed by the application of the PPAR‑γ antagonist GW9662. In addition, combined treatment with GW9662 and DHA increased NF‑κB‑related protein expression. These results indicate that DHA regulates MMP‑9 expression and cell invasion via modulation of the MAPK signaling pathway and PPAR‑γ/NF‑κB activity. This suggests that DHA could be a potential therapeutic agent for the prevention of breast cancer metastasis.
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January-2017
Volume 13 Issue 1

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Hwang JK, Yu HN, Noh EM, Kim JM, Hong OY, Youn HJ, Jung SH, Kwon KB, Kim JS, Lee YR, Lee YR, et al: DHA blocks TPA-induced cell invasion by inhibiting MMP-9 expression via suppression of the PPAR‑γ/NF-κB pathway in MCF-7 cells. Oncol Lett 13: 243-249, 2017
APA
Hwang, J., Yu, H., Noh, E., Kim, J., Hong, O., Youn, H.J. ... Lee, Y. (2017). DHA blocks TPA-induced cell invasion by inhibiting MMP-9 expression via suppression of the PPAR‑γ/NF-κB pathway in MCF-7 cells. Oncology Letters, 13, 243-249. https://doi.org/10.3892/ol.2016.5382
MLA
Hwang, J., Yu, H., Noh, E., Kim, J., Hong, O., Youn, H. J., Jung, S. H., Kwon, K., Kim, J., Lee, Y."DHA blocks TPA-induced cell invasion by inhibiting MMP-9 expression via suppression of the PPAR‑γ/NF-κB pathway in MCF-7 cells". Oncology Letters 13.1 (2017): 243-249.
Chicago
Hwang, J., Yu, H., Noh, E., Kim, J., Hong, O., Youn, H. J., Jung, S. H., Kwon, K., Kim, J., Lee, Y."DHA blocks TPA-induced cell invasion by inhibiting MMP-9 expression via suppression of the PPAR‑γ/NF-κB pathway in MCF-7 cells". Oncology Letters 13, no. 1 (2017): 243-249. https://doi.org/10.3892/ol.2016.5382