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Article

H19 promotes endometrial cancer progression by modulating epithelial-mesenchymal transition

  • Authors:
    • Le Zhao
    • Zhen Li
    • Wei Chen
    • Wen Zhai
    • Jingjing Pan
    • Huan Pang
    • Xu Li
  • View Affiliations / Copyright

    Affiliations: Center for Translational Medicine, The First Affiliated Hospital, Xi'an Jiaotong University, Xi'an, Shaanxi 710061, P.R. China, Department of Gynecology and Obstetrics, The First Affiliated Hospital, Xi'an Jiaotong University, Xi'an, Shaanxi 710061, P.R. China, Center for Laboratory Medicine, The First Affiliated Hospital, Xi'an Jiaotong University, Xi'an, Shaanxi 710061, P.R. China
  • Pages: 363-369
    |
    Published online on: November 16, 2016
       https://doi.org/10.3892/ol.2016.5389
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Abstract

Endometrial cancer is one of the most common types of gynecological malignancy worldwide. Novel biomarkers and therapeutic targets are imperative for improving patients' survival. Previous studies have suggested the long non‑coding RNA H19 as a potential cancer biomarker. To investigate the role of H19 in endometrial cancer, the present study examined the expression pattern of H19 in endometrial cancer tissues by quantitative polymerase chain reaction, and characterized its function in the endometrial cancer cell line via knocking down its expression with small interfering RNAs. It was found that H19 level was significantly higher in tumor tissues than in paratumoral tissues. Knockdown of H19 did not affect the growth rate of HEC‑1‑B endometrial cancer cells, but significantly suppressed in vitro migration and invasion of HEC‑1‑B cells. Furthermore, H19 downregulation decreased Snail level and increased E‑cadherin expression without affecting vimentin level, indicating partial reversion of epithelial‑mesenchymal transition (EMT). The present findings suggested that H19 contributed to the aggressiveness of endometrial cancer by modulating EMT process.
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1 

Ferlay J, Soerjomataram I, Dikshit R, Eser S, Mathers C, Rebelo M, Parkin DM, Forman D and Bray F: Cancer incidence and mortality worldwide: Sources, methods and major patterns in GLOBOCAN 2012. Int J Cancer. 136:E359–E386. 2015. View Article : Google Scholar : PubMed/NCBI

2 

Weiderpass E, Antoine J, Bray FI, Oh JK and Arbyn M: Trends in corpus uteri cancer mortality in member states of the European Union. Eur J Cancer. 50:1675–1684. 2014. View Article : Google Scholar : PubMed/NCBI

3 

Duong LM, Wilson RJ, Ajani UA, Singh SD and Eheman CR: Trends in endometrial cancer incidence rates in the United States, 1999–2006. J Womens Health (Larchmt). 20:1157–1163. 2011. View Article : Google Scholar : PubMed/NCBI

4 

Edwards BK, Noone AM, Mariotto AB, Simard EP, Boscoe FP, Henley SJ, Jemal A, Cho H, Anderson RN, Kohler BA, et al: Annual Report to the Nation on the status of cancer, 1975–2010, featuring prevalence of comorbidity and impact on survival among persons with lung, colorectal, breast, or prostate cancer. Cancer. 120:1290–1314. 2014. View Article : Google Scholar : PubMed/NCBI

5 

Li X, Zheng S, Chen S, Qin F, Lau S and Chen Q: Trends in gynaecological cancers in the largest obstetrics and gynaecology hospital in China from 2003 to 2013. Tumour Biol. 36:4961–4966. 2015. View Article : Google Scholar : PubMed/NCBI

6 

Bansal N, Yendluri V and Wenham RM: The molecular biology of endometrial cancers and the implications for pathogenesis, classification and targeted therapies. Cancer Control. 16:8–13. 2009.PubMed/NCBI

7 

Bokhman JV: Two pathogenetic types of endometrial carcinoma. Gynecol Oncol. 15:10–17. 1983. View Article : Google Scholar : PubMed/NCBI

8 

Setiawan VW, Yang HP, Pike MC, McCann SE, Yu H, Xiang YB, Wolk A, Wentzensen N, Weiss NS, Webb PM, et al: Type I and II endometrial cancers: Have they different risk factors? J Clin Oncol. 31:2607–2618. 2013. View Article : Google Scholar : PubMed/NCBI

9 

Zemel S, Bartolomei MS and Tilghman SM: Physical linkage of two mammalian imprinted genes, H19 and insulin-like growth factor 2. Nat Genet. 2:61–65. 1992. View Article : Google Scholar : PubMed/NCBI

10 

Zhang Y and Tycko B: Monoallelic expression of the human H19 gene. Nat Genet. 1:40–44. 1992. View Article : Google Scholar : PubMed/NCBI

11 

Pachnis V, Brannan CI and Tilghman SM: The structure and expression of a novel gene activated in early mouse embryogenesis. EMBO J. 7:673–681. 1988.PubMed/NCBI

12 

Hao Y, Crenshaw T, Moulton T, Newcomb E and Tycko B: Tumour-suppressor activity of H19 RNA. Nature. 365:764–767. 1993. View Article : Google Scholar : PubMed/NCBI

13 

Zhu M, Chen Q, Liu X, Sun Q, Zhao X, Deng R, Wang Y, Huang J, Xu M, Yan J and Yu J: lncRNA H19/miR-675 axis represses prostate cancer metastasis by targeting TGFBI. FEBS J. 281:3766–3775. 2014. View Article : Google Scholar : PubMed/NCBI

14 

Berteaux N, Lottin S, Monté D, Pinte S, Quatannens B, Coll J, Hondermarck H, Curgy JJ, Dugimont T and Adriaenssens E: H19 mRNA-like noncoding RNA promotes breast cancer cell proliferation through positive control by E2F1. J Biol Chem. 280:29625–29636. 2005. View Article : Google Scholar : PubMed/NCBI

15 

Medrzycki M, Zhang Y, Zhang W, Cao K, Pan C, Lailler N, McDonald JF, Bouhassira EE and Fan Y: Histone h1.3 suppresses h19 noncoding RNA expression and cell growth of ovarian cancer cells. Cancer Res. 74:6463–6473. 2014. View Article : Google Scholar : PubMed/NCBI

16 

Matouk IJ, Raveh E, Abu-lail R, Mezan S, Gilon M, Gershtain E, Birman T, Gallula J, Schneider T, Barkali M, et al: Oncofetal H19 RNA promotes tumor metastasis. Biochim Biophys Acta. 1843:1414–1426. 2014. View Article : Google Scholar : PubMed/NCBI

17 

Ma C, Nong K, Zhu H, Wang W, Huang X, Yuan Z and Ai K: H19 promotes pancreatic cancer metastasis by derepressing let-7's suppression on its target HMGA2-mediated EMT. Tumour Biol. 35:9163–9169. 2014. View Article : Google Scholar : PubMed/NCBI

18 

Tanos V, Ariel I, Prus D, De-Groot N and Hochberg A: H19 and IGF2 gene expression in human normal, hyperplastic and malignant endometrium. Int J Gynecol Cancer. 14:521–525. 2004. View Article : Google Scholar : PubMed/NCBI

19 

Lottin S, Adriaenssens E, Berteaux N, Lepretrê A, Vilain MO, Denhez E, Coll J, Dugimont T and Curgy JJ: The human H19 gene is frequently overexpressed in myometrium and stroma during pathological endometrial proliferative events. Eur J Cancer. 41:168–177. 2005. View Article : Google Scholar : PubMed/NCBI

20 

Mokhtar NM, Ramzi NH, Yin-Ling W, Rose IM, Mohd Dali AZ Hatta and Jamal R: Laser capture microdissection with genome-wide expression profiling displayed gene expression signatures in endometrioid endometrial cancer. Cancer Invest. 30:156–164. 2012. View Article : Google Scholar : PubMed/NCBI

21 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(−Delta Delta C(T)) Method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

22 

Siegel RL, Miller KD and Jemal A: Cancer statistics, 2015. CA Cancer J Clin. 65:5–29. 2015. View Article : Google Scholar : PubMed/NCBI

23 

Chen W, Zheng R, Baade PD, Zhang S, Zeng H, Bray F, Jemal A, Yu XQ and He J: Cancer statistics in China, 2015. CA Cancer J Clin. 66:115–132. 2016. View Article : Google Scholar : PubMed/NCBI

24 

Matouk IJ, DeGroot N, Mezan S, Ayesh S, Abu-lail R, Hochberg A and Galun E: The H19 non-coding RNA is essential for human tumor growth. PloS one. 2:e8452007. View Article : Google Scholar : PubMed/NCBI

25 

Wang L, Sun Y, Yi J, Wang X, Liang J, Pan Z, Li L and Jiang G: Targeting H19 by lentivirus-mediated RNA interference increases A549 cell migration and invasion. Exp Lung Res. 1–8. 2016.(Epub ahead of print).

26 

Zhang E, Li W, Yin D, De W, Zhu L, Sun S and Han L: c-Myc-regulated long non-coding RNA H19 indicates a poor prognosis and affects cell proliferation in non-small-cell lung cancer. Tumour Biol. 37:4007–4015. 2016. View Article : Google Scholar : PubMed/NCBI

27 

Yoshimizu T, Miroglio A, Ripoche MA, Gabory A, Vernucci M, Riccio A, Colnot S, Godard C, Terris B, Jammes H and Dandolo L: The H19 locus acts in vivo as a tumor suppressor. Proc Natl Acad Sci USA. 105:12417–12422. 2008. View Article : Google Scholar : PubMed/NCBI

28 

Wang L, Cai Y, Zhao X, Jia X, Zhang J, Liu J, Zhen H, Wang T, Tang X, Liu Y and Wang J: Down-regulated long non-coding RNA H19 inhibits carcinogenesis of renal cell carcinoma. Neoplasma. 62:412–418. 2015. View Article : Google Scholar : PubMed/NCBI

29 

Xia T, Liao Q, Jiang X, Shao Y, Xiao B, Xi Y and Guo J: Long noncoding RNA associated-competing endogenous RNAs in gastric cancer. Sci Rep. 4:60882014.PubMed/NCBI

30 

Hernandez JM, Elahi A, Clark CW, Wang J, Humphries LA, Centeno B, Bloom G, Fuchs BC, Yeatman T and Shibata D: miR-675 mediates downregulation of Twist1 and Rb in AFP-secreting hepatocellular carcinoma. Ann Surg Oncol. 20:Suppl 3. S625–S635. 2013. View Article : Google Scholar : PubMed/NCBI

31 

Guo G, Kang Q, Chen Q, Chen Z, Wang J, Tan L and Chen JL: High expression of long non-coding RNA H19 is required for efficient tumorigenesis induced by Bcr-Abl oncogene. FEBS Lett. 588:1780–1786. 2014. View Article : Google Scholar : PubMed/NCBI

32 

Min HY, Lee SC, Woo JK, Jung HJ, Park KH, Jeong HM, Hyun SY, Cho J, Lee W, Park JE, et al: Essential role of DNA methyltransferase 1-mediated transcription of insulin-like growth factor 2 in resistance to histone deacetylase inhibitors. Clin Cancer Res. pii: clincanres.0534.2016, 2016. (Epub ahead of print).

33 

Dugimont T, Montpellier C, Adriaenssens E, Lottin S, Dumont L, Iotsova V, Lagrou C, Stéhelin D, Coll J and Curgy JJ: The H19 TATA-less promoter is efficiently repressed by wild-type tumor suppressor gene product p53. Oncogene. 16:2395–2401. 1998. View Article : Google Scholar : PubMed/NCBI

34 

Luo M, Li Z, Wang W, Zeng Y, Liu Z and Qiu J: Long non-coding RNA H19 increases bladder cancer metastasis by associating with EZH2 and inhibiting E-cadherin expression. Cancer Lett. 333:213–221. 2013. View Article : Google Scholar : PubMed/NCBI

35 

Zhang L, Yang F, Yuan JH, Yuan SX, Zhou WP, Huo XS, Xu D, Bi HS, Wang F and Sun SH: Epigenetic activation of the MiR-200 family contributes to H19-mediated metastasis suppression in hepatocellular carcinoma. Carcinogenesis. 34:577–586. 2013. View Article : Google Scholar : PubMed/NCBI

36 

Zhang EB, Han L, Yin DD, Kong R, De W and Chen J: c-Myc-induced, long, noncoding H19 affects cell proliferation and predicts a poor prognosis in patients with gastric cancer. Med Oncol. 31:9142014. View Article : Google Scholar : PubMed/NCBI

37 

Yang F, Bi J, Xue X, Zheng L, Zhi K, Hua J and Fang G: Up-regulated long non-coding RNA H19 contributes to proliferation of gastric cancer cells. FEBS J. 279:3159–3165. 2012. View Article : Google Scholar : PubMed/NCBI

38 

Ayesh S, Matouk I, Schneider T, Ohana P, Laster M, Al-Sharef W, De-Groot N and Hochberg A: Possible physiological role of H19 RNA. Mol Carcinog. 35:63–74. 2002. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Zhao L, Li Z, Chen W, Zhai W, Pan J, Pang H and Li X: H19 promotes endometrial cancer progression by modulating epithelial-mesenchymal transition. Oncol Lett 13: 363-369, 2017.
APA
Zhao, L., Li, Z., Chen, W., Zhai, W., Pan, J., Pang, H., & Li, X. (2017). H19 promotes endometrial cancer progression by modulating epithelial-mesenchymal transition. Oncology Letters, 13, 363-369. https://doi.org/10.3892/ol.2016.5389
MLA
Zhao, L., Li, Z., Chen, W., Zhai, W., Pan, J., Pang, H., Li, X."H19 promotes endometrial cancer progression by modulating epithelial-mesenchymal transition". Oncology Letters 13.1 (2017): 363-369.
Chicago
Zhao, L., Li, Z., Chen, W., Zhai, W., Pan, J., Pang, H., Li, X."H19 promotes endometrial cancer progression by modulating epithelial-mesenchymal transition". Oncology Letters 13, no. 1 (2017): 363-369. https://doi.org/10.3892/ol.2016.5389
Copy and paste a formatted citation
x
Spandidos Publications style
Zhao L, Li Z, Chen W, Zhai W, Pan J, Pang H and Li X: H19 promotes endometrial cancer progression by modulating epithelial-mesenchymal transition. Oncol Lett 13: 363-369, 2017.
APA
Zhao, L., Li, Z., Chen, W., Zhai, W., Pan, J., Pang, H., & Li, X. (2017). H19 promotes endometrial cancer progression by modulating epithelial-mesenchymal transition. Oncology Letters, 13, 363-369. https://doi.org/10.3892/ol.2016.5389
MLA
Zhao, L., Li, Z., Chen, W., Zhai, W., Pan, J., Pang, H., Li, X."H19 promotes endometrial cancer progression by modulating epithelial-mesenchymal transition". Oncology Letters 13.1 (2017): 363-369.
Chicago
Zhao, L., Li, Z., Chen, W., Zhai, W., Pan, J., Pang, H., Li, X."H19 promotes endometrial cancer progression by modulating epithelial-mesenchymal transition". Oncology Letters 13, no. 1 (2017): 363-369. https://doi.org/10.3892/ol.2016.5389
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