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The effect of miR-146a on STAT1 expression and apoptosis in acute lymphoblastic leukemia Jurkat cells

  • Authors:
    • Weihong Yan
    • Hua Guo
    • Feng Suo
    • Chunling Han
    • Hua Zheng
    • Tong Chen
  • View Affiliations / Copyright

    Affiliations: Department of Pediatrics, Dongying People's Hospital, Dongying, Shangdong 257091, P.R. China, Department of Radiology, Dongying People's Hospital, Dongying, Shangdong 257091, P.R. China
    Copyright: © Yan et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 151-154
    |
    Published online on: November 18, 2016
       https://doi.org/10.3892/ol.2016.5395
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Abstract

The effect of miR-146a-dependent regulation of STAT1 on apoptosis in acute lymphoblastic leukemia (ALL) Jurkat cells was investigated. The miR-146a mimic and miR-146a inhibitor vectors were constructed in vitro, and experimental grouping was as follows: Control group (untreated Jurkat cells), empty vector group (Jurkat cells transfected with empty vector), agonist group (Jurkat cells transfected with miR-146a mimic) and the inhibitor group (Jurkat cells transfected with miR-146a inhibitor). Western blot analysis was used to observe the expression, respectively, of STAT1, p-STAT1 and Bcl-xL, and flow cytometry was used to test apoptosis in Jurkat cells. STAT1 and p-STAT1 expression in the agonist group was higher than that in the control and empty vector groups, but lower in the inhibitor group, and differences were statistically significant (P<0.05). The rate of apoptosis in the agonist group was significantly higher than that of the control group and blank vector group, and it was significantly lower in the inhibitor group (P<0.05). As a tumor suppressor, miR-146a can regulate expression of apoptosis‑promoting factor STAT1, and anti-apoptosis factor Bcl-xL, and is able to promote apoptosis of ALL Jurkat cells.
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1 

Ye EA and Steinle JJ: miR-146a attenuates inflammatory pathways mediated by TLR4/NF-κB and TNFα to protect primary human retinal microvascular endothelial cells grown in high glucose. Mediators Inflamm. 2016:39584532016. View Article : Google Scholar : PubMed/NCBI

2 

Czajka AA, Wójcicka A, Kubiak A, Kotlarek M, Bakuła-Zalewska E, Koperski Ł, Wiechno W and Jażdżewski K: Family of microRNA-146 regulates RARβ in papillary thyroid carcinoma. PLoS One. 11:e01519682016. View Article : Google Scholar : PubMed/NCBI

3 

Lin Y, Zhang Q, Zhang HM, Liu W, Liu CJ, Li Q and Guo AY: Transcription factor and miRNA co-regulatory network reveals shared and specific regulators in the development of B cell and T cell. Sci Rep. 5:152152015. View Article : Google Scholar : PubMed/NCBI

4 

Zhang H, Luo XQ, Zhang P, Huang LB, Zheng YS, Wu J, Zhou H, Qu LH, Xu L and Chen YQ: MicroRNA patterns associated with clinical prognostic parameters and CNS relapse prediction in pediatric acute leukemia. PLoS One. 4:e78262009. View Article : Google Scholar : PubMed/NCBI

5 

Adamaki M, Tsotra M, Vlahopoulos S, Zampogiannis A, Papavassiliou AG and Moschovi M: STAT transcript levels in childhood acute lymphoblastic leukemia: STAT1 and STAT3 transcript correlations. Leuk Res. Sep 11–2015.(Epub ahead of print). View Article : Google Scholar

6 

Lucas CM, Milani M, Butterworth M, Carmell N, Scott LJ, Clark RE, Cohen GM and Varadarajan S: High CIP2A levels correlate with an antiapoptotic phenotype that can be overcome by targeting BCL-XL in chronic myeloid leukemia. Leukemia. Feb 29–2016.(Epub ahead of print). View Article : Google Scholar

7 

Xu L, Zhong H, Wan H, Chen FY, Zhong J, Xiao F, Liu J and Shen L: miR-146a expression level as a novel putative prognostic marker for acute promyelocytic leukemia. Dis Markers. 2014:1506042014. View Article : Google Scholar : PubMed/NCBI

8 

Duyu M, Durmaz B, Gunduz C, Vergin C, Karapinar D Yilmaz, Aksoylar S, Kavakli K, Cetingul N, Irken G, Yaman Y, et al: Prospective evaluation of whole genome microRNA expression profiling in childhood acute lymphoblastic leukemia. Biomed Res Int. 2014:9675852014. View Article : Google Scholar : PubMed/NCBI

9 

Saki N, Abroun S, Soleimani M, Mortazavi Y, Kaviani S and Arefian E: The roles of miR-146a in the differentiation of Jurkat T-lymphoblasts. Hematology. 19:141–147. 2014. View Article : Google Scholar : PubMed/NCBI

10 

Lionetti M, Musto P, Di Martino MT, Fabris S, Agnelli L, Todoerti K, Tuana G, Mosca L, Cantafio ME Gallo, Grieco V, et al: Biological and clinical relevance of miRNA expression signatures in primary plasma cell leukemia. Clin Cancer Res. 19:3130–3142. 2013. View Article : Google Scholar : PubMed/NCBI

11 

Spinello I, Quaranta MT, Riccioni R, Riti V, Pasquini L, Boe A, Pelosi E, Vitale A, Foà R, Testa U, et al: MicroRNA-146a and AMD3100, two ways to control CXCR4 expression in acute myeloid leukemias. Blood Cancer J. 1:e262011. View Article : Google Scholar : PubMed/NCBI

12 

Zhao C, Wang W, Yu W, Jou D, Wang Y, Ma H, Xiao H, Qin H, Zhang C, Lü J, et al: A novel small molecule STAT3 inhibitor, LY5, inhibits cell viability, colony formation, and migration of colon and liver cancer cells. Oncotarget. 7:12917–12926. 2016.PubMed/NCBI

13 

Midgley AC, Morris G, Phillips AO and Steadman R: 17β-estradiol ameliorates age-associated loss of fibroblast function by attenuating IFN-γ/STAT1-dependent miR-7 upregulation. Aging Cell. 15:531–541. 2016. View Article : Google Scholar : PubMed/NCBI

14 

Legrier ME, Bièche I, Gaston J, Beurdeley A, Yvonnet V, Déas O, Thuleau A, Château-Joubert S, Servely JL, Vacher S, et al: Activation of IFN/STAT1 signalling predicts response to chemotherapy in oestrogen receptor-negative breast cancer. Br J Cancer. 114:177–187. 2016. View Article : Google Scholar : PubMed/NCBI

15 

Moles R, Bellon M and Nicot C: STAT1: A novel target of miR-150 and miR-223 is involved in the proliferation of HTLV-I-transformed and ATL cells. Neoplasia. 17:449–462. 2015. View Article : Google Scholar : PubMed/NCBI

16 

Xu D, Han Q, Hou Z, Zhang C and Zhang J: miR-146a negatively regulates NK cell functions via STAT1 signaling. Cell Mol Immunol. Mar 21–2016.(Epub ahead of print). View Article : Google Scholar

17 

Wang S, Zhang X, Ju Y, Zhao B, Yan X, Hu J, Shi L, Yang L, Ma Z, Chen L, et al: MicroRNA-146a feedback suppresses T cell immune function by targeting Stat1 in patients with chronic hepatitis B. J Immunol. 191:293–301. 2013. View Article : Google Scholar : PubMed/NCBI

18 

Kutty RK, Nagineni CN, Samuel W, Vijayasarathy C, Jaworski C, Duncan T, Cameron JE, Flemington EK, Hooks JJ and Redmond TM: Differential regulation of microRNA-146a and microRNA-146b-5p in human retinal pigment epithelial cells by interleukin-1β, tumor necrosis factor-α, and interferon-γ. Mol Vis. 19:737–750. 2013.PubMed/NCBI

19 

Gény C, Rivière G, Bignon J, Birlirakis N, Guittet E, Awang K, Litaudon M, Roussi F and Dumontet V: Anacardic acids from Knema hookeriana as modulators of Bcl-xL/Bak and Mcl-1/Bid interactions. J Nat Prod. 79:838–844. 2016. View Article : Google Scholar : PubMed/NCBI

20 

Sarosiek KA and Letai A: Directly targeting the mitochondrial pathway of apoptosis for cancer therapy with BH3 mimetics: recent successes, current challenges and future promise. FEBS J. Mar 21–2016.(Epub ahead of print). View Article : Google Scholar : PubMed/NCBI

21 

Matsumoto M, Nakajima W, Seike M, Gemma A and Tanaka N: Cisplatin-induced apoptosis in non-small-cell lung cancer cells is dependent on Bax- and Bak-induction pathway and synergistically activated by BH3-mimetic ABT-263 in p53 wild-type and mutant cells. Biochem Biophys Res Commun. 473:490–496. 2016. View Article : Google Scholar : PubMed/NCBI

22 

Hennessy EJ: Selective inhibitors of Bcl-2 and Bcl-xL: balancing antitumor activity with on-target toxicity. Bioorg Med Chem Lett. 26:2105–2114. 2016. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Yan W, Guo H, Suo F, Han C, Zheng H and Chen T: The effect of miR-146a on STAT1 expression and apoptosis in acute lymphoblastic leukemia Jurkat cells. Oncol Lett 13: 151-154, 2017.
APA
Yan, W., Guo, H., Suo, F., Han, C., Zheng, H., & Chen, T. (2017). The effect of miR-146a on STAT1 expression and apoptosis in acute lymphoblastic leukemia Jurkat cells. Oncology Letters, 13, 151-154. https://doi.org/10.3892/ol.2016.5395
MLA
Yan, W., Guo, H., Suo, F., Han, C., Zheng, H., Chen, T."The effect of miR-146a on STAT1 expression and apoptosis in acute lymphoblastic leukemia Jurkat cells". Oncology Letters 13.1 (2017): 151-154.
Chicago
Yan, W., Guo, H., Suo, F., Han, C., Zheng, H., Chen, T."The effect of miR-146a on STAT1 expression and apoptosis in acute lymphoblastic leukemia Jurkat cells". Oncology Letters 13, no. 1 (2017): 151-154. https://doi.org/10.3892/ol.2016.5395
Copy and paste a formatted citation
x
Spandidos Publications style
Yan W, Guo H, Suo F, Han C, Zheng H and Chen T: The effect of miR-146a on STAT1 expression and apoptosis in acute lymphoblastic leukemia Jurkat cells. Oncol Lett 13: 151-154, 2017.
APA
Yan, W., Guo, H., Suo, F., Han, C., Zheng, H., & Chen, T. (2017). The effect of miR-146a on STAT1 expression and apoptosis in acute lymphoblastic leukemia Jurkat cells. Oncology Letters, 13, 151-154. https://doi.org/10.3892/ol.2016.5395
MLA
Yan, W., Guo, H., Suo, F., Han, C., Zheng, H., Chen, T."The effect of miR-146a on STAT1 expression and apoptosis in acute lymphoblastic leukemia Jurkat cells". Oncology Letters 13.1 (2017): 151-154.
Chicago
Yan, W., Guo, H., Suo, F., Han, C., Zheng, H., Chen, T."The effect of miR-146a on STAT1 expression and apoptosis in acute lymphoblastic leukemia Jurkat cells". Oncology Letters 13, no. 1 (2017): 151-154. https://doi.org/10.3892/ol.2016.5395
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