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Article

Transcription factor Oct4 promotes osteosarcoma by regulating lncRNA AK055347

  • Authors:
    • Hongwu Fan
    • Guangyao Liu
    • Changfu Zhao
    • Xuefeng Li
    • Xiaoyu Yang
  • View Affiliations / Copyright

    Affiliations: Department of Orthopedics, China‑Japan Union Hospital of Jilin University, Changchun, Jilin 130001, P.R. China, Department of Anesthesiology, China‑Japan Union Hospital of Jilin University, Changchun, Jilin 130001, P.R. China, Department of Orthopedics, Orthopedics Hospital, The Second Hospital of Jilin University, Changchun, Jilin 130001, P.R. China
  • Pages: 396-402
    |
    Published online on: November 21, 2016
       https://doi.org/10.3892/ol.2016.5400
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Abstract

Osteosarcoma is the most common primary bone tumor in children and adolescents, typically presenting with a poor prognosis. Octamer-binding transcription factor 4 (Oct4) protein, encoded by the POU class 5 homeobox 1 gene, is important in maintaining self-renewal of pluripotent stem cells, and is closely associated with cancer. However, its role in osteosarcoma remains to be elucidated. The present study observed Oct4 was markedly increased in osteosarcoma cell lines and in human osteosarcoma tissue samples. Following Oct4 downregulation by small interfering RNA (siRNA) in osteosarcoma F5M2 cells, the cells exhibited significant decreases in proliferation and invasion ability, and an increase in cell apoptosis. Notably, downregulation of Oct4 decreased the expression of AK055347, a newly identified long noncoding RNA (lncRNA) in human tissues. The downregulation of AK055347 by siRNA resulted in a significant suppressive effect on proliferative and invasive ability, and promotion of cell apoptosis in osteosarcoma cells. Thus, the current study suggests Oct4 exerts a promoting effect in osteosarcoma, and identifies a novel lncRNA in osteosarcoma progression.
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Copy and paste a formatted citation
Spandidos Publications style
Fan H, Liu G, Zhao C, Li X and Yang X: Transcription factor Oct4 promotes osteosarcoma by regulating lncRNA AK055347. Oncol Lett 13: 396-402, 2017.
APA
Fan, H., Liu, G., Zhao, C., Li, X., & Yang, X. (2017). Transcription factor Oct4 promotes osteosarcoma by regulating lncRNA AK055347. Oncology Letters, 13, 396-402. https://doi.org/10.3892/ol.2016.5400
MLA
Fan, H., Liu, G., Zhao, C., Li, X., Yang, X."Transcription factor Oct4 promotes osteosarcoma by regulating lncRNA AK055347". Oncology Letters 13.1 (2017): 396-402.
Chicago
Fan, H., Liu, G., Zhao, C., Li, X., Yang, X."Transcription factor Oct4 promotes osteosarcoma by regulating lncRNA AK055347". Oncology Letters 13, no. 1 (2017): 396-402. https://doi.org/10.3892/ol.2016.5400
Copy and paste a formatted citation
x
Spandidos Publications style
Fan H, Liu G, Zhao C, Li X and Yang X: Transcription factor Oct4 promotes osteosarcoma by regulating lncRNA AK055347. Oncol Lett 13: 396-402, 2017.
APA
Fan, H., Liu, G., Zhao, C., Li, X., & Yang, X. (2017). Transcription factor Oct4 promotes osteosarcoma by regulating lncRNA AK055347. Oncology Letters, 13, 396-402. https://doi.org/10.3892/ol.2016.5400
MLA
Fan, H., Liu, G., Zhao, C., Li, X., Yang, X."Transcription factor Oct4 promotes osteosarcoma by regulating lncRNA AK055347". Oncology Letters 13.1 (2017): 396-402.
Chicago
Fan, H., Liu, G., Zhao, C., Li, X., Yang, X."Transcription factor Oct4 promotes osteosarcoma by regulating lncRNA AK055347". Oncology Letters 13, no. 1 (2017): 396-402. https://doi.org/10.3892/ol.2016.5400
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