Recognition of tumor antigens in 4T1 cells by natural IgM from three strains of mice with different susceptibilities to spontaneous breast cancer

Díaz‑Zaragoza,Mariana; Hernández‑Ávila,Ricardo; Ostoa‑Saloma,Pedro

doi:10.3892/ol.2016.5427

January-2017 Volume 13 Issue 1

Full Size Image

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

An International Open Access Journal Devoted to General Medicine.

January-2017 Volume 13 Issue 1

Full Size Image

Article

Recognition of tumor antigens in 4T1 cells by natural IgM from three strains of mice with different susceptibilities to spontaneous breast cancer

Authors:
- Mariana Díaz‑Zaragoza
- Ricardo Hernández‑Ávila
- Pedro Ostoa‑Saloma
View Affiliations / Copyright

Affiliations: Departamento de Inmunología, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, 04510 Ciudad de México, México
Pages: 271-274
|
Published online on: November 23, 2016

https://doi.org/10.3892/ol.2016.5427
Expand metrics +

Abstract

The issue of antibody responses to tumors is potentially important to cancer immunologists. Early detection of cancer represents one of the most promising approaches to reduce the growing cancer burden. Natural immunoglobulin (Ig)M antibodies have been associated with the recognition and elimination of cancerous and precancerous cells. Using natural IgM antibodies, the present study identified a set of antigens in healthy mice from three different strains and examined whether the global patterns of antibodies are able to discriminate between a condition of more or less susceptibility to breast cancer. The current study performed two‑dimensional (2D) immunoblotting to detect antigens from 4T1 cells using natural IgM from serum of healthy female mice from three different strains. The t‑test was used to analyze the total number of spots. There were no significant differences in the numbers of antigens recognized in each strain. However, differences in patterns were observed on 2D immunoblots among the three strains. The reactivity patterns of natural IgM antibodies to particular antigens exhibited non‑random clustering, which discriminated between strains with different susceptibilities to spontaneous breast cancer. The results demonstrated that the patterns of reactivity to defined subsets of antigens are able to provide information regarding differential diagnosis associated with breast cancer sensitivity. Therefore, it may be concluded that it is possible to segregate the IgM humoral immune response toward cancer antigens according to the genetic background of individuals. In addition, it is possible to identify the recognized antigens that allow grouping or discriminate between the different IgM antibodies expressed. The possible association between a particular antigen and cancer susceptibility requires further study, but the methodology exposed in the present study may identify potential candidates for this possible association.

View Figures

View References

1	Tabernero MD, Lv LL and Anderson KS: Autoantibody profiles as biomarkers of breast cancer. Cancer Biomark. 6:247–256. 2010.PubMed/NCBI
2	Tan HT, Low J, Lim SG and Chung MC: Serum autoantibodies as biomarkers for early cancer detection. FEBS J. 276:6880–6904. 2009. View Article : Google Scholar : PubMed/NCBI
3	Desmetz C, Bascoul-Mollevi C, Rochaix P, Lamy PJ, Kramar A, Rouanet P, Maudelonde T, Mangé A and Solassol J: Identification of a new panel of serum autoantibodies associated with the presence of in situ carcinoma of the breast in younger women. Clin Cancer Res. 15:4733–4741. 2009. View Article : Google Scholar : PubMed/NCBI
4	Anderson KS, Ramachandran N, Wong J, Raphael JV, Hainsworth E, Demirkan G, Cramer D, Aronzon D, Hodi FS, Harris L, et al: Application of protein microarrays for multiplexed detection of antibodies to tumor antigens in breast cancer. J Proteome Res. 7:1490–1499. 2008. View Article : Google Scholar : PubMed/NCBI
5	Tan EM and Zhang J: Autoantibodies to tumor-associated antigens: Reporters from the immune system. Immunol Rev. 222:328–340. 2008. View Article : Google Scholar : PubMed/NCBI
6	Lu H, Goodell V and Disis ML: Humoral immunity directed against tumor-associated-antigens as potential biomarkers for the early diagnosis of cancer. J Proteome Res. 7:1388–1394. 2008. View Article : Google Scholar : PubMed/NCBI
7	Finn OJ: Immune response as a biomarker for cancer detection and a lot more. N Engl J Med. 353:1288–1290. 2005. View Article : Google Scholar : PubMed/NCBI
8	Storr SJ, Chakrabarti J, Barnes A, Murray A, Chapman CJ and Robertson JF: Use of autoantibodies in breast cancer screening and diagnosis. Expert Rev Anticancer Ther. 6:1215–1223. 2006. View Article : Google Scholar : PubMed/NCBI
9	Anderson KS and LaBaer J: The sentinel within: Exploiting the immune system for cancer biomarkers. J Proteome Res. 4:1123–1133. 2005. View Article : Google Scholar : PubMed/NCBI
10	Vollmers HP and Brändlein S: Natural antibodies and cancer. N Biotechnol. 25:294–298. 2009. View Article : Google Scholar : PubMed/NCBI
11	Díaz-Zaragoza M, Hernández-Ávila R, Viedma-Rodríguez R, Arenas-Aranda D and Ostoa-Saloma P: Natural and adaptive IgM antibodies in the recognition of tumor-associated antigens of breast cancer (Review). Oncol Rep. 34:1106–1114. 2015.PubMed/NCBI
12	Díaz-Zaragoza M, Hernández R and Ostoa-Saloma P: 2D immunoblots show differential response of mouse IgG and IgM antibodies to antigens of mammary carcinoma 4 T1 cells. Cancer Cell Int. 14:92014. View Article : Google Scholar : PubMed/NCBI
13	Díaz-Zaragoza M, Hernández-Ávila R, Govezensky T, Mendoza L, Meneses-Ruíz DM and Ostoa-Saloma P: Comparison patterns of 4 T1 antigens recognized by humoral immune response mediated by IgG and IgM antibodies in female and male mice with breast cancer using 2D-immnunoblots. Immunobiology. 220:1050–1058. 2015. View Article : Google Scholar : PubMed/NCBI
14	Hoag WG: Spontaneous cancer in mice. Ann N Y Acad Sci. 108:805–831. 1963. View Article : Google Scholar : PubMed/NCBI
15	Eads D: hcluster 0.2.0: A hierarcial clustering package for Scipy. https://pypi.python.org/pypi/hcluster/0.2.0Accessed March 4, 2016.
16	Nobrega A, Haury M, Grandien A, Malanchère E, Sundblad A and Coutinho A: Global analysis of antibody repertoires. II. Evidence for specificity, self-selection and the immunological ‘homunculus’ of antibodies in normal serum. Eur J Immunol. 23:2851–2859. 1993. View Article : Google Scholar : PubMed/NCBI

Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Recognition of tumor antigens in 4T1 cells by natural IgM from three strains of mice with different susceptibilities to spontaneous breast cancer

This article is mentioned in:

Abstract

Figure 1

Figure 2

Figure 3

Figure 4

Related Articles