Per2 participates in AKT-mediated drug resistance in A549/DDP lung adenocarcinoma cells

  • Authors:
    • Bo Chen
    • Yaoxi Tan
    • Yan Liang
    • Yan Li
    • Lei Chen
    • Shuangshuang Wu
    • Wei Xu
    • Yan Wang
    • Weihong Zhao
    • Jianqing Wu
  • View Affiliations

  • Published online on: November 24, 2016     https://doi.org/10.3892/ol.2016.5430
  • Pages: 423-428
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Abstract

Period2 (Per2) is a key mammalian circadian clock protein, and additionally has a tumor suppressive function. The present study aimed to investigate its role in drug resistance in A549/cisplatin (DDP) lung adenocarcinoma cells. Per2 knockdown and overexpression in A549/DDP cells were used to compare cell proliferation (by MTT assay), apoptosis (active‑caspase 3 western blot) and clone forming assay. The activation of AKT/mechanistic target of rapamycin (mTOR) was investigated by a western blot assay. The Per2 expression level was decreased in A549/DDP cells compared with A549 cells. Per2 knockdown by short hairpin RNA protects A549/DDP cells from apoptosis, and promotes proliferation and migration. Per2 knockdown results in increased activation of the phosphoinositide 3‑kinase (PI3K)/AKT/mTOR signaling pathway. Overexpression of Per2 in A549/DDP cells may reduce the activity of the PI3K/AKT/mTOR signaling pathway, and promote apoptosis of A549 cells. The results of the present study suggest that Per2 participates in AKT-mediated drug resistance in A549/DDP lung adenocarcinoma cells.
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January-2017
Volume 13 Issue 1

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Chen B, Tan Y, Liang Y, Li Y, Chen L, Wu S, Xu W, Wang Y, Zhao W, Wu J, Wu J, et al: Per2 participates in AKT-mediated drug resistance in A549/DDP lung adenocarcinoma cells. Oncol Lett 13: 423-428, 2017
APA
Chen, B., Tan, Y., Liang, Y., Li, Y., Chen, L., Wu, S. ... Wu, J. (2017). Per2 participates in AKT-mediated drug resistance in A549/DDP lung adenocarcinoma cells. Oncology Letters, 13, 423-428. https://doi.org/10.3892/ol.2016.5430
MLA
Chen, B., Tan, Y., Liang, Y., Li, Y., Chen, L., Wu, S., Xu, W., Wang, Y., Zhao, W., Wu, J."Per2 participates in AKT-mediated drug resistance in A549/DDP lung adenocarcinoma cells". Oncology Letters 13.1 (2017): 423-428.
Chicago
Chen, B., Tan, Y., Liang, Y., Li, Y., Chen, L., Wu, S., Xu, W., Wang, Y., Zhao, W., Wu, J."Per2 participates in AKT-mediated drug resistance in A549/DDP lung adenocarcinoma cells". Oncology Letters 13, no. 1 (2017): 423-428. https://doi.org/10.3892/ol.2016.5430