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Article

Hypoxia regulates CD44 expression via hypoxia-inducible factor-1α in human gastric cancer cells

  • Authors:
    • Gai Liang
    • Shuang Li
    • Wei Du
    • Qinghua Ke
    • Jun Cai
    • Jiyuan Yang
  • View Affiliations / Copyright

    Affiliations: Department of Oncology, First Affiliated Hospital of Yangtze University, Jingzhou, Hubei 434000, P.R. China
  • Pages: 967-972
    |
    Published online on: December 8, 2016
       https://doi.org/10.3892/ol.2016.5473
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Abstract

Hypoxia induces proliferation and invasion in cancer cells via hypoxia-inducible factor (HIF)-1α. The cell adhesion molecule cluster of differentiation (CD) 44 has been associated with increased cell invasion and metastasis. Whether hypoxia regulates the expression of CD44 in gastric cancer cells remains to be established. In the current study, the effects of hypoxia on HIF‑1α and CD44 expression levels in human gastric cell lines SGC‑7901 and BGC‑823 were evaluated. The cells were cultured in 1% O2 for 1 week and then treated with 20 nM rapamycin for 72 h. Cell viability was evaluated using the Cell Counting kit‑8 assay, and cell invasion was detected by the Transwell invasion assay. The protein and messenger (m) RNA expression levels of HIF‑1α and CD44 were detected using western blotting and reverse transcription‑quantitative polymerase chain reaction, respectively. The results revealed that cell viability and invasion increased under hypoxic conditions, but decreased following rapamycin treatment in SGC‑7901 and BGC‑823 cells. Hypoxia also increased the protein and mRNA expression levels of HIF‑1α and CD44 in these two cell lines. However, this hypoxia‑induced increase in HIF‑1α and CD44 protein and mRNA expression levels was inhibited by rapamycin. These findings suggest that hypoxia induced the proliferation and invasion of SGC‑7901 and BGC‑823 cells. Furthermore, CD44 expression levels were potentially associated with HIF‑1α expression levels. Therefore, in gastric cancer cells, hypoxia may regulate CD44 expression via HIF‑1α in order to promote cell proliferation and invasion.
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Copy and paste a formatted citation
Spandidos Publications style
Liang G, Li S, Du W, Ke Q, Cai J and Yang J: Hypoxia regulates CD44 expression via hypoxia-inducible factor-1α in human gastric cancer cells. Oncol Lett 13: 967-972, 2017.
APA
Liang, G., Li, S., Du, W., Ke, Q., Cai, J., & Yang, J. (2017). Hypoxia regulates CD44 expression via hypoxia-inducible factor-1α in human gastric cancer cells. Oncology Letters, 13, 967-972. https://doi.org/10.3892/ol.2016.5473
MLA
Liang, G., Li, S., Du, W., Ke, Q., Cai, J., Yang, J."Hypoxia regulates CD44 expression via hypoxia-inducible factor-1α in human gastric cancer cells". Oncology Letters 13.2 (2017): 967-972.
Chicago
Liang, G., Li, S., Du, W., Ke, Q., Cai, J., Yang, J."Hypoxia regulates CD44 expression via hypoxia-inducible factor-1α in human gastric cancer cells". Oncology Letters 13, no. 2 (2017): 967-972. https://doi.org/10.3892/ol.2016.5473
Copy and paste a formatted citation
x
Spandidos Publications style
Liang G, Li S, Du W, Ke Q, Cai J and Yang J: Hypoxia regulates CD44 expression via hypoxia-inducible factor-1α in human gastric cancer cells. Oncol Lett 13: 967-972, 2017.
APA
Liang, G., Li, S., Du, W., Ke, Q., Cai, J., & Yang, J. (2017). Hypoxia regulates CD44 expression via hypoxia-inducible factor-1α in human gastric cancer cells. Oncology Letters, 13, 967-972. https://doi.org/10.3892/ol.2016.5473
MLA
Liang, G., Li, S., Du, W., Ke, Q., Cai, J., Yang, J."Hypoxia regulates CD44 expression via hypoxia-inducible factor-1α in human gastric cancer cells". Oncology Letters 13.2 (2017): 967-972.
Chicago
Liang, G., Li, S., Du, W., Ke, Q., Cai, J., Yang, J."Hypoxia regulates CD44 expression via hypoxia-inducible factor-1α in human gastric cancer cells". Oncology Letters 13, no. 2 (2017): 967-972. https://doi.org/10.3892/ol.2016.5473
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