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Article

Overexpression of KAI1 inhibits retinoblastoma metastasis in vitro

  • Authors:
    • Hui Yan
    • Xunda Ji
    • Jing Li
    • Lei Zhang
    • Peiquan Zhao
  • View Affiliations / Copyright

    Affiliations: Department of Ophthalmology, Xinhua Hospital Affiliated to Shanghai Jiaotong University School of Medicine, Shanghai 200092, P.R. China, Department of Ophthalmology, Tongji University Affiliated Yangpu Hospital, Shanghai 200090, P.R. China
  • Pages: 827-833
    |
    Published online on: December 16, 2016
       https://doi.org/10.3892/ol.2016.5507
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Abstract

The present study aimed to investigate the expression of cluster of differentiation 82 (KAI1), a gene involved in the suppression of tumor metastasis, in human retinoblastoma (RB) tissue and to study the effect of KAI1 expression on RB cell migration and invasion. KAI1 expression was examined in 26 patients with non‑invasive and invasive retinoblastoma using reverse transcription‑quantitative polymerase chain reaction and western blot analysis. A lentiviral vector containing KAI1 cDNA was used to transfect the two RB cell lines, HXO‑Rb44‑Gl and Y79. Following successful transfection, the migratory and invasive capacity of the two RB cell lines was evaluated using a Transwell® migration assay. KAI1 expression was observed to be downregulated in invasive RB compared to non‑invasive RB. The migratory and invasive capacities of KAI1 transfected cell lines were significantly decreased compared to those of the control cells. KAI1 may be involved in retinoblastoma metastasis, and increased expression of KAI1 significantly inhibits the metastatic ability of RB cells in vitro.
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1 

Kiss S, Leiderman YI and Mukai S: Diagnosis, classification, and treatment of retinoblastoma. Int Ophthalmol Clin. 48:135–147. 2008. View Article : Google Scholar : PubMed/NCBI

2 

Liu Q, Wang Y, Wang H, Liu Y, Liu T and Kunda PE: Tandem therapy for retinoblastoma: Immunotherapy and chemotherapy enhance cytotoxicity on retinoblastoma by increasing apoptosis. J Cancer Res Clin Oncol. 139:1357–1372. 2013. View Article : Google Scholar : PubMed/NCBI

3 

Shields CL and Shields JA: Basic understanding of current classification and management of retinoblastoma. Curr Opin Ophthalmol. 17:228–234. 2006. View Article : Google Scholar : PubMed/NCBI

4 

de Moura LR, Marshall JC, Di Cesare S, Fernandes BF, Antecka E and Burnier MN: The effect of imatinib mesylate on the proliferation, invasive ability, and radiosensitivity of retinoblastoma cell lines. Eye (Lond). 27:92–99. 2013. View Article : Google Scholar : PubMed/NCBI

5 

Chawla B, Hasan F, Azad R, Seth R, Upadhyay AD, Pathy S and Pandey RM: Clinical presentation and survival of retinoblastoma in Indian children. Br J Ophthalmol. 100:172–178. 2016. View Article : Google Scholar : PubMed/NCBI

6 

Dunkel IJ, Khakoo Y, Kernan NA, Gershon T, Gilheeney S, Lyden DC, Wolden SL, Orjuela M, Gardner SL and Abramson DH: Intensive multimodality therapy for patients with stage 4a metastatic retinoblastoma. Pediatr Blood Cancer. 55:55–59. 2010.PubMed/NCBI

7 

Dong JT, Lamb PW, RinkerSchaeffer CW, Vukanovic J, Ichikawa T, Isaacs JT and Barrett JC: KAI1, a metastasis suppressor gene for prostate cancer on human chromosome 11p11.2. Science. 268:884–886. 1995. View Article : Google Scholar : PubMed/NCBI

8 

Park JJ, Jin YB, Lee YJ, Lee JS, Lee YS, Ko YG and Lee M: KAI1 suppresses HIF-1α and VEGF expression by blocking CDCP1-enhanced Src activation in prostate cancer. BMC Cancer. 12:812012. View Article : Google Scholar : PubMed/NCBI

9 

Lee HA, Park I, Byun HJ, Jeoung D, Kim YM and Lee H: Metastasis suppressor KAI1/CD82 attenuates the matrix adhesion of human prostate cancer cells by suppressing fibronectin expression and β1 integrin activation. Cell Physiol Biochem. 27:575–586. 2011. View Article : Google Scholar : PubMed/NCBI

10 

Gellersen B, Wolf A, Kruse M, Schwenke M and Bamberger AM: Human endometrial stromal cell-trophoblast interactions: Mutual stimulation of chemotactic migration and promigratory roles of cell surface molecules CD82 and CEACAM1. Biol Reprod. 88:802013. View Article : Google Scholar : PubMed/NCBI

11 

Feng J, Huang C, Wren JD, Wang DW, Yan J, Zhang J, Sun Y, Han X and Zhang XA: Tetraspanin CD82: A suppressor of solid tumors and a modulator of membrane heterogeneity. Cancer Metastasis Rev. 34:619–633. 2015. View Article : Google Scholar : PubMed/NCBI

12 

Bouras T and Frauman AG: Expression of the prostate cancer metastasis suppressor gene KAI1 in primary prostate cancers: A biphasic relationship with tumour grade. J Pathol. 188:382–388. 1999. View Article : Google Scholar : PubMed/NCBI

13 

Mooez S, Malik FA, Kayani MA, Rashid R, Zahid A and Khan A: Expressional alterations and transcript isoforms of metastasis suppressor genes (KAI1 and KiSS1) in breast cancer patients. Asian Pac J Cancer Prev. 12:2785–2791. 2011.PubMed/NCBI

14 

Friess H, Guo XZ, BeRBerat P, Graber HU, Zimmermann A, Korc M and Büchler MW: Reduced KAI1 expression in pancreatic cancer is associated with lymph node and distant metastases. Int J Cancer. 79:349–355. 1998. View Article : Google Scholar : PubMed/NCBI

15 

Shiwu WU, Lan Y, Wenqing S, Lei Z and Yisheng T: Expression and clinical significance of CD82/KAI1 and E-cadherin in non-small cell lung cancer. Arch Iran Med. 15:707–712. 2012.PubMed/NCBI

16 

Lakshmi S Amirtha, Pushparaj V, Krishnamurthy V, Biswas J, Krishnakumar S and Shanmugam MP: Tetraspanin protein KAI1 expression in retinoblastoma. Br J Ophthalmol. 88:593–595. 2004. View Article : Google Scholar : PubMed/NCBI

17 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(−Delta Delta C (T)) Method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

18 

Ji X, Zhang J, Cheng L, Wei F, Li H, Liu X, Chen X, Li C, Wang Y and Huang Q: Oncolytic adenovirus delivering herpes simplex virus thymidine kinase suicide gene reduces the growth of human retinoblastoma in an in vivo mouse model. Exp Eye Res. 89:193–199. 2009. View Article : Google Scholar : PubMed/NCBI

19 

Xu H, Wang C, Zhu H, Liu S, Xu X and Jiang Y: Characteristics of an established retinoblastoma cell line HXO-Rb44. Yan Ke Xue Bao. 11:16–21. 1995.PubMed/NCBI

20 

Lv K, Guo Y, Zhang Y, Wang K, Li K, Zhu Y and Sun S: Transient inhibition of foot-and-mouth disease virus replication by siRNAs silencing VP1 protein coding region. Res Vet Sci. 86:443–452. 2009. View Article : Google Scholar : PubMed/NCBI

21 

Li M, Husic N, Lin Y and Snider BJ: Production of lentiviral vectors for transducing cells from the central nervous system. J Vis Exp. 24:e40312012.

22 

Ji XD, Yan H and Zhao PQ: Expression changes of tumor metastasis-related genes after overexpression of KAI1 in retinoblastoma Y79 cells. Zhonghua Yan Ke Za Zhi. 48:1097–1101. 2012.(In Chinese). PubMed/NCBI

23 

Zhang B, Liu W, Li L, Lu J, Liu M, Sun Y and Jin D: KAI1/CD82 and Cyclin D1 as biomarkers of invasion, metastasis and prognosis of laryngeal squamous cell carcinoma. Int J Clin Exp Pathol. 6:1060–1067. 2013.PubMed/NCBI

24 

Yang X, Wei LL, Tang C, Slack R, Mueller S and Lippman ME: Overexpression of KAI1 suppresses in vitro invasiveness and in vivo metastasis in breast cancer cells. Cancer Res. 61:5284–5288. 2001.PubMed/NCBI

25 

Xu JH, Guo XZ, Ren LN, Shao LC and Liu MP: KAI1 is a potential target for anti-metastasis in pancreatic cancer cells. World J Gastroenterol. 14:1126–1132. 2008. View Article : Google Scholar : PubMed/NCBI

26 

Ruseva Z, Geiger PX, Hutzler P, Kotzsch M, Luber B, Schmitt M, Gross E and Reuning U: Tumor suppressor KAI1 affects integrin alphavbeta3-mediated ovarian cancer cell adhesion, motility, and proliferation. Exp Cell Res. 315:1759–1771. 2009. View Article : Google Scholar : PubMed/NCBI

27 

Lombardi DP, Geradts J, Foley JF, Chiao C, Lamb PW and Barrett JC: Loss of KAI1 expression in the progression of colorectal cancer. Cancer Res. 59:5724–5731. 1999.PubMed/NCBI

28 

Stark AM, Tongers K, Maass N, Mehdorn HM and Held-Feindt J: Reduced metastasis-suppressor gene mRNA-expression in breast cancer brain metastases. J Cancer Res Clin Oncol. 131:191–198. 2005. View Article : Google Scholar : PubMed/NCBI

29 

Malik FA, Sanders AJ, Kayani MA and Jiang WG: Effect of expressional alteration of KAI1 on breast cancer cell growth, adhesion, migration and invasion. Cancer Genomics Proteomics. 6:205–213. 2009.PubMed/NCBI

30 

Liu X, Guo XZ, Zhang WW, Lu ZZ, Zhang QW, Duan HF and Wang LS: KAI1 inhibits HGF-induced invasion of pancreatic cancer by sphingosine kinase activity. Hepatobiliary Pancreat Dis Int. 10:201–208. 2011. View Article : Google Scholar : PubMed/NCBI

31 

Yang JM, Peng ZH, Si SH, Liu WW, Luo YH and Ye ZY: KAI1 gene suppresses invasion and metastasis of hepatocellular carcinoma MHCC97-H cells in vitro and in animal models. Liver Int. 28:132–139. 2008. View Article : Google Scholar : PubMed/NCBI

32 

Liu WM and Zhang XA: KAI1/CD82, a tumor metastasis suppressor. Cancer Lett. 240:183–194. 2006. View Article : Google Scholar : PubMed/NCBI

33 

Miranti CK: Controlling cell surface dynamics and signaling: How CD82/KAI1 supresses metastasis. Cell Signal. 21:196–211. 2009. View Article : Google Scholar : PubMed/NCBI

34 

Abe M, Sugiura T, Takahashi M, Ishii K, Shimoda M and Shirasuna K: A novel function of CD82/KAI-1 on E-cadherin-mediated homophilic cellular adhesion of cancer cells. Cancer Lett. 266:163–170. 2008. View Article : Google Scholar : PubMed/NCBI

35 

Singh A and Settleman J: EMT, cancer stem cells and drug resistance: An emerging axis of evil in the war on cancer. Oncogene. 29:4741–4751. 2010. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Yan H, Ji X, Li J, Zhang L and Zhao P: Overexpression of KAI1 inhibits retinoblastoma metastasis in vitro. Oncol Lett 13: 827-833, 2017.
APA
Yan, H., Ji, X., Li, J., Zhang, L., & Zhao, P. (2017). Overexpression of KAI1 inhibits retinoblastoma metastasis in vitro. Oncology Letters, 13, 827-833. https://doi.org/10.3892/ol.2016.5507
MLA
Yan, H., Ji, X., Li, J., Zhang, L., Zhao, P."Overexpression of KAI1 inhibits retinoblastoma metastasis in vitro". Oncology Letters 13.2 (2017): 827-833.
Chicago
Yan, H., Ji, X., Li, J., Zhang, L., Zhao, P."Overexpression of KAI1 inhibits retinoblastoma metastasis in vitro". Oncology Letters 13, no. 2 (2017): 827-833. https://doi.org/10.3892/ol.2016.5507
Copy and paste a formatted citation
x
Spandidos Publications style
Yan H, Ji X, Li J, Zhang L and Zhao P: Overexpression of KAI1 inhibits retinoblastoma metastasis in vitro. Oncol Lett 13: 827-833, 2017.
APA
Yan, H., Ji, X., Li, J., Zhang, L., & Zhao, P. (2017). Overexpression of KAI1 inhibits retinoblastoma metastasis in vitro. Oncology Letters, 13, 827-833. https://doi.org/10.3892/ol.2016.5507
MLA
Yan, H., Ji, X., Li, J., Zhang, L., Zhao, P."Overexpression of KAI1 inhibits retinoblastoma metastasis in vitro". Oncology Letters 13.2 (2017): 827-833.
Chicago
Yan, H., Ji, X., Li, J., Zhang, L., Zhao, P."Overexpression of KAI1 inhibits retinoblastoma metastasis in vitro". Oncology Letters 13, no. 2 (2017): 827-833. https://doi.org/10.3892/ol.2016.5507
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