Trametes robiniophila may induce apoptosis and inhibit MMPs expression in the human gastric carcinoma cell line MKN‑45

  • Authors:
    • Xuening Ji
    • Chunxia Pan
    • Xiaowen Li
    • Yunbin Gao
    • Lu Xia
    • Xiulian Quan
    • Jinyan Lv
    • Ruoyu Wang
  • View Affiliations

  • Published online on: December 20, 2016     https://doi.org/10.3892/ol.2016.5517
  • Pages: 841-846
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Abstract

Gastric carcinoma (GC) is one of the most common malignant tumors and is mainly treated by invasive surgeries. The present study aimed to investigate the treatment potential of Trametes robiniophila on GC using the human GC cell line MKN‑45. Cells were incubated with Trametes robiniophila at a concentration of 0, 5 and 10 mg/ml for 24 h. The apoptosis of the cell line was examined with acridine orange/ethidium bromide staining and flow cytometry. The expression of B-cell lymphoma (Bcl)‑2, Fas, caspase‑3, matrix metalloproteinase (MMP)‑2 and MMP‑9 was analyzed using reverse transcription‑polymerase chain reaction and western blotting. With increasing drug concentrations, the proportion of apoptotic and necrotic cells increased. For a certain concentration, the apoptotic ratio also increased with increasing response times. Compared with the control group, the Bcl‑2, MMP‑2 and MMP‑9 expression levels in the MKN‑45 cell line decreased, while the expression levels of Fas and caspase‑3 increased (P<0.05), and the expression patterns were strengthened with increasing drug concentrations. The present study revealed that Trametes robiniophila had treatment potential on GC, and it may act on gastric cells through apoptotic induction and MMPs expression inhibition. Based on the present results, Trametes robiniophila may be considered as an alternative approach for noninvasive therapy of GC. However, future studies should be performed to clarify this further.
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February-2017
Volume 13 Issue 2

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Ji X, Pan C, Li X, Gao Y, Xia L, Quan X, Lv J and Wang R: Trametes robiniophila may induce apoptosis and inhibit MMPs expression in the human gastric carcinoma cell line MKN‑45. Oncol Lett 13: 841-846, 2017
APA
Ji, X., Pan, C., Li, X., Gao, Y., Xia, L., Quan, X. ... Wang, R. (2017). Trametes robiniophila may induce apoptosis and inhibit MMPs expression in the human gastric carcinoma cell line MKN‑45. Oncology Letters, 13, 841-846. https://doi.org/10.3892/ol.2016.5517
MLA
Ji, X., Pan, C., Li, X., Gao, Y., Xia, L., Quan, X., Lv, J., Wang, R."Trametes robiniophila may induce apoptosis and inhibit MMPs expression in the human gastric carcinoma cell line MKN‑45". Oncology Letters 13.2 (2017): 841-846.
Chicago
Ji, X., Pan, C., Li, X., Gao, Y., Xia, L., Quan, X., Lv, J., Wang, R."Trametes robiniophila may induce apoptosis and inhibit MMPs expression in the human gastric carcinoma cell line MKN‑45". Oncology Letters 13, no. 2 (2017): 841-846. https://doi.org/10.3892/ol.2016.5517