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Article

Repurposing cimetidine for cholangiocarcinoma: Antitumor effects in vitro and in vivo

  • Authors:
    • Paweena Dana
    • Kulthida Vaeteewoottacharn
    • Ryusho Kariya
    • Kouki Matsuda
    • Sopit Wongkham
    • Seiji Okada
  • View Affiliations / Copyright

    Affiliations: Department of Biochemistry, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand, Division of Hematopoiesis, Center for AIDS Research, Kumamoto University, Kumamoto 860‑0811, Japan
  • Pages: 1432-1436
    |
    Published online on: January 2, 2017
       https://doi.org/10.3892/ol.2017.5563
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Abstract

Cimetidine is a histamine type-2 (H2) receptor antagonist that has been demonstrated to have antitumor effects on various types of malignancy. However, its effect on cholangiocarcinoma (CCA), a chemotherapy‑resistant bile duct tumor, has yet to be investigated. In the present study, the antitumor activity of cimetidine in vitro and in vivo was evaluated. A methylthiotetrazole assay revealed that the proliferation of certain CCA cell lines was inhibited by cimetidine, which induced the caspase‑dependent apoptosis of CCA cells via suppression of the protein kinase B signaling pathway. Suppression of Akt phosphorylation, caspase‑3, ‑8 and ‑9 activation, phosphotidylserine exposure determined by Annexin V binding assay and the presence of a sub‑G1 population were demonstrated by western blotting and flow cytometry analysis. In a CCA xenograft mouse model cimetidine inhibited the growth of CCA cells without observable adverse effects. These results suggest that cimetidine has the potential to be an effective antitumor agent for the treatment of CCA.
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Copy and paste a formatted citation
Spandidos Publications style
Dana P, Vaeteewoottacharn K, Kariya R, Matsuda K, Wongkham S and Okada S: Repurposing cimetidine for cholangiocarcinoma: Antitumor effects in vitro and in vivo. Oncol Lett 13: 1432-1436, 2017.
APA
Dana, P., Vaeteewoottacharn, K., Kariya, R., Matsuda, K., Wongkham, S., & Okada, S. (2017). Repurposing cimetidine for cholangiocarcinoma: Antitumor effects in vitro and in vivo. Oncology Letters, 13, 1432-1436. https://doi.org/10.3892/ol.2017.5563
MLA
Dana, P., Vaeteewoottacharn, K., Kariya, R., Matsuda, K., Wongkham, S., Okada, S."Repurposing cimetidine for cholangiocarcinoma: Antitumor effects in vitro and in vivo". Oncology Letters 13.3 (2017): 1432-1436.
Chicago
Dana, P., Vaeteewoottacharn, K., Kariya, R., Matsuda, K., Wongkham, S., Okada, S."Repurposing cimetidine for cholangiocarcinoma: Antitumor effects in vitro and in vivo". Oncology Letters 13, no. 3 (2017): 1432-1436. https://doi.org/10.3892/ol.2017.5563
Copy and paste a formatted citation
x
Spandidos Publications style
Dana P, Vaeteewoottacharn K, Kariya R, Matsuda K, Wongkham S and Okada S: Repurposing cimetidine for cholangiocarcinoma: Antitumor effects in vitro and in vivo. Oncol Lett 13: 1432-1436, 2017.
APA
Dana, P., Vaeteewoottacharn, K., Kariya, R., Matsuda, K., Wongkham, S., & Okada, S. (2017). Repurposing cimetidine for cholangiocarcinoma: Antitumor effects in vitro and in vivo. Oncology Letters, 13, 1432-1436. https://doi.org/10.3892/ol.2017.5563
MLA
Dana, P., Vaeteewoottacharn, K., Kariya, R., Matsuda, K., Wongkham, S., Okada, S."Repurposing cimetidine for cholangiocarcinoma: Antitumor effects in vitro and in vivo". Oncology Letters 13.3 (2017): 1432-1436.
Chicago
Dana, P., Vaeteewoottacharn, K., Kariya, R., Matsuda, K., Wongkham, S., Okada, S."Repurposing cimetidine for cholangiocarcinoma: Antitumor effects in vitro and in vivo". Oncology Letters 13, no. 3 (2017): 1432-1436. https://doi.org/10.3892/ol.2017.5563
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