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Article

Proliferation of sphere‑forming hepatocellular carcinoma cells is suppressed in a medium without glucose and arginine, but with galactose and ornithine

  • Authors:
    • Minoru Tomizawa
    • Fuminobu Shinozaki
    • Yasufumi Motoyoshi
    • Takao Sugiyama
    • Shigenori Yamamoto
    • Naoki Ishige
  • View Affiliations / Copyright

    Affiliations: Department of Gastroenterology, National Hospital Organization, Shimoshizu Hospital, Yotsukaido, Chiba 284‑0003, Japan, Department of Radiology, National Hospital Organization, Shimoshizu Hospital, Yotsukaido, Chiba 284‑0003, Japan, Department of Neurology, National Hospital Organization, Shimoshizu Hospital, Yotsukaido, Chiba 284‑0003, Japan, Department of Rheumatology, National Hospital Organization, Shimoshizu Hospital, Yotsukaido, Chiba 284‑0003, Japan, Department of Pediatrics, National Hospital Organization, Shimoshizu Hospital, Yotsukaido, Chiba 284‑0003, Japan, Department of Neurosurgery, National Hospital Organization, Shimoshizu Hospital, Yotsukaido, Chiba 284‑0003, Japan
  • Pages: 1264-1268
    |
    Published online on: January 2, 2017
       https://doi.org/10.3892/ol.2017.5565
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Abstract

Resistance to sorafenib in hepatocellular carcinoma (HCC) cells exhibiting stemness was evaluated using a sphere formation assay. A hepatocyte selection medium (HSM) deficient in glucose and arginine was used to suppress the proliferation of cell spheres composed of HLF and PLC/PRF/5 HCC cells, which were subjected to a sphere formation assay. Cell spheres were cultured with sorafenib and subjected to a cell proliferation assay and the expression levels of cytochrome P450 (CYP3A4) were analyzed in RNA extracted from sphere‑forming cells using reverse transcription‑quantitative polymerase chain reaction. Sphere‑forming PLC/PRF/5 cells were more resistant to sorafenib, as compared with control cells, exhibiting higher expression levels of CYP3A4. When cultured in HSM, suppressed proliferation was observed in the sphere‑forming PLC/PRF/5 cells and in the control cells, with no significant variation between them. The results suggest that deprivation of glucose and arginine is a potential novel treatment for HCC.
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Copy and paste a formatted citation
Spandidos Publications style
Tomizawa M, Shinozaki F, Motoyoshi Y, Sugiyama T, Yamamoto S and Ishige N: Proliferation of sphere‑forming hepatocellular carcinoma cells is suppressed in a medium without glucose and arginine, but with galactose and ornithine. Oncol Lett 13: 1264-1268, 2017.
APA
Tomizawa, M., Shinozaki, F., Motoyoshi, Y., Sugiyama, T., Yamamoto, S., & Ishige, N. (2017). Proliferation of sphere‑forming hepatocellular carcinoma cells is suppressed in a medium without glucose and arginine, but with galactose and ornithine. Oncology Letters, 13, 1264-1268. https://doi.org/10.3892/ol.2017.5565
MLA
Tomizawa, M., Shinozaki, F., Motoyoshi, Y., Sugiyama, T., Yamamoto, S., Ishige, N."Proliferation of sphere‑forming hepatocellular carcinoma cells is suppressed in a medium without glucose and arginine, but with galactose and ornithine". Oncology Letters 13.3 (2017): 1264-1268.
Chicago
Tomizawa, M., Shinozaki, F., Motoyoshi, Y., Sugiyama, T., Yamamoto, S., Ishige, N."Proliferation of sphere‑forming hepatocellular carcinoma cells is suppressed in a medium without glucose and arginine, but with galactose and ornithine". Oncology Letters 13, no. 3 (2017): 1264-1268. https://doi.org/10.3892/ol.2017.5565
Copy and paste a formatted citation
x
Spandidos Publications style
Tomizawa M, Shinozaki F, Motoyoshi Y, Sugiyama T, Yamamoto S and Ishige N: Proliferation of sphere‑forming hepatocellular carcinoma cells is suppressed in a medium without glucose and arginine, but with galactose and ornithine. Oncol Lett 13: 1264-1268, 2017.
APA
Tomizawa, M., Shinozaki, F., Motoyoshi, Y., Sugiyama, T., Yamamoto, S., & Ishige, N. (2017). Proliferation of sphere‑forming hepatocellular carcinoma cells is suppressed in a medium without glucose and arginine, but with galactose and ornithine. Oncology Letters, 13, 1264-1268. https://doi.org/10.3892/ol.2017.5565
MLA
Tomizawa, M., Shinozaki, F., Motoyoshi, Y., Sugiyama, T., Yamamoto, S., Ishige, N."Proliferation of sphere‑forming hepatocellular carcinoma cells is suppressed in a medium without glucose and arginine, but with galactose and ornithine". Oncology Letters 13.3 (2017): 1264-1268.
Chicago
Tomizawa, M., Shinozaki, F., Motoyoshi, Y., Sugiyama, T., Yamamoto, S., Ishige, N."Proliferation of sphere‑forming hepatocellular carcinoma cells is suppressed in a medium without glucose and arginine, but with galactose and ornithine". Oncology Letters 13, no. 3 (2017): 1264-1268. https://doi.org/10.3892/ol.2017.5565
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