Monocyte‑derived dendritic cells from patients with cervical intraepithelial lesions

  • Authors:
    • Angela Maria Moed Lopes
    • Márcia Antoniazi Michelin
    • Eddie Fernando Cândido Murta
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  • Published online on: January 11, 2017     https://doi.org/10.3892/ol.2017.5595
  • Pages: 1456-1462
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Abstract

Immunotherapy with dendritic cells (DCs) is a great promise for the treatment of neoplasms. However, the obtainment and protocol of differentiation of these cells may depend on extrinsic factors such as the tumor itself. The aim of the present study was to verify the influence of cervical neoplasia on different protocols of differentiation of monocyte‑derived DCs resulting in an increased maturation phenotype. A total of 83 women were included in the study. The patients were grouped in low‑grade squamous intraepithelial lesion (LSIL) (n=30), high‑grade squamous intraepithelial lesion (HSIL) (n=22), cervical cancer (n=10) and healthy patients (n=21) groups. The mononuclear cells of patients were subjected to three differentiation protocols. In protocol I (pI), granulocyte‑macrophage colony‑stimulating factor (GM‑CSF), interleukin (IL)‑4 and tumor necrosis factor (TNF)‑α were used for the differentiation of mature DCs (pIDCs). In protocol II (pII), monocytes were stimulated with GM‑CSF, IL‑4, TNF‑α and activated lymphocytes in the absence of non‑adherent cells (pIIDCs). In protocol III (pIII), monocytes were stimulated with GM‑CSF, IL‑4, TNF‑α and activated lymphocytes in the presence of non‑adherent cells (pIIIDCs). These cells were evaluated by flow cytometry for the expression of maturation markers such as cluster of differentiation (CD)11c, CD86 and human leukocyte antigen‑antigen D related (HLA‑DR). The main cytokines secreted (IL‑4, IL‑12 and transforming growth factor‑β) were measured by ELISA. Our results indicate a significantly lower mature profile of pIIDCs and a significant increase in CD11c+ pIIIDCs able to produce IL‑12 (P=0.0007). Furthermore, a significant reduction in cervical cancer HLA‑DR+ pIDCs (P=0.0113) was also observed. HSIL patients exhibited a higher percentage of HLA‑DR+ pIIDCs (P=0.0113), while LSIL patients had a lower percentage of CD11c+ pIIIDCs (P=0.0411). These findings suggest that the extent of cervical lesions affects the process of differentiation of DCs. Furthermore, activated lymphocytes may induce a better maturation of monocyte‑derived DCs, and the presence of mononuclear cells appears to contribute to the DC differentiation process.
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March-2017
Volume 13 Issue 3

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Lopes AM, Michelin MA and Murta EF: Monocyte‑derived dendritic cells from patients with cervical intraepithelial lesions. Oncol Lett 13: 1456-1462, 2017
APA
Lopes, A.M., Michelin, M.A., & Murta, E.F. (2017). Monocyte‑derived dendritic cells from patients with cervical intraepithelial lesions. Oncology Letters, 13, 1456-1462. https://doi.org/10.3892/ol.2017.5595
MLA
Lopes, A. M., Michelin, M. A., Murta, E. F."Monocyte‑derived dendritic cells from patients with cervical intraepithelial lesions". Oncology Letters 13.3 (2017): 1456-1462.
Chicago
Lopes, A. M., Michelin, M. A., Murta, E. F."Monocyte‑derived dendritic cells from patients with cervical intraepithelial lesions". Oncology Letters 13, no. 3 (2017): 1456-1462. https://doi.org/10.3892/ol.2017.5595