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Association between hyperpolarization-activated channel in interstitial cells of Cajal and gastrointestinal dysmotility induced by malignant ascites

  • Authors:
    • Tieyun Guo
    • Jiade Li
    • Jing Li
    • Dan Kong
    • Chunli Bi
    • Zheng He
    • Dai Tang
    • Xiaoming Jin
    • Lianhong Jin
  • View Affiliations / Copyright

    Affiliations: Department of Histology and Embryology, Basic Medical Science College, Harbin Medical University, Harbin, Heilongjiang 150081, P.R. China, Department of Pathology, Basic Medical Science College, Harbin Medical University, Harbin, Heilongjiang 150081, P.R. China, Department of Gynecology, The Third Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang 150081, P.R. China
    Copyright: © Guo et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1601-1608
    |
    Published online on: January 25, 2017
       https://doi.org/10.3892/ol.2017.5652
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Abstract

Advanced malignant ascites is accompanied by gastrointestinal dysmotility, and patients often feel abdominal pain, abdominal distention, nausea and constipation. Gastrointestinal dysmotility is not only painful for the patients, but it reduces the absorption of nutrients and affects the physical recovery of patients with malignant ascites. It is reported that changes in interstitial cells of Cajal (ICCs) are responsible for the gastrointestinal dysmotility induced by malignant ascites, but the mechanism is not completely understood. The present study observed a significantly decreased expression of ion channels, including hyperpolarization‑activated cyclic nucleotide‑gated potassium channel 2 (HCN2) and cyclic adenosine monophosphate, in the condition of malignant ascites. Using electrophysiology, it was identified that malignant ascites led to lower amplitude and slower frequency signals in cells of the small intestine. In addition, when ICCs were cultured with malignant ascites in vitro, the expression of HCN2 of ICCs was significantly reduced, and the data of flow cytometry revealed that the Ca2+ concentration of ICCs was also decreased. The results of electron microscopy analysis demonstrated the nuclei of ICCs were pyknotic, and the processes of ICCs were reduced in malignant ascites. The present study suggests the small intestinal dysmotility caused by malignant ascites may be associated with changes in HCN2 of ICCs, which offers a potential therapeutic target for gastrointestinal dysmotility in advanced malignant ascites.
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Copy and paste a formatted citation
Spandidos Publications style
Guo T, Li J, Li J, Kong D, Bi C, He Z, Tang D, Jin X and Jin L: Association between hyperpolarization-activated channel in interstitial cells of Cajal and gastrointestinal dysmotility induced by malignant ascites. Oncol Lett 13: 1601-1608, 2017.
APA
Guo, T., Li, J., Li, J., Kong, D., Bi, C., He, Z. ... Jin, L. (2017). Association between hyperpolarization-activated channel in interstitial cells of Cajal and gastrointestinal dysmotility induced by malignant ascites. Oncology Letters, 13, 1601-1608. https://doi.org/10.3892/ol.2017.5652
MLA
Guo, T., Li, J., Li, J., Kong, D., Bi, C., He, Z., Tang, D., Jin, X., Jin, L."Association between hyperpolarization-activated channel in interstitial cells of Cajal and gastrointestinal dysmotility induced by malignant ascites". Oncology Letters 13.3 (2017): 1601-1608.
Chicago
Guo, T., Li, J., Li, J., Kong, D., Bi, C., He, Z., Tang, D., Jin, X., Jin, L."Association between hyperpolarization-activated channel in interstitial cells of Cajal and gastrointestinal dysmotility induced by malignant ascites". Oncology Letters 13, no. 3 (2017): 1601-1608. https://doi.org/10.3892/ol.2017.5652
Copy and paste a formatted citation
x
Spandidos Publications style
Guo T, Li J, Li J, Kong D, Bi C, He Z, Tang D, Jin X and Jin L: Association between hyperpolarization-activated channel in interstitial cells of Cajal and gastrointestinal dysmotility induced by malignant ascites. Oncol Lett 13: 1601-1608, 2017.
APA
Guo, T., Li, J., Li, J., Kong, D., Bi, C., He, Z. ... Jin, L. (2017). Association between hyperpolarization-activated channel in interstitial cells of Cajal and gastrointestinal dysmotility induced by malignant ascites. Oncology Letters, 13, 1601-1608. https://doi.org/10.3892/ol.2017.5652
MLA
Guo, T., Li, J., Li, J., Kong, D., Bi, C., He, Z., Tang, D., Jin, X., Jin, L."Association between hyperpolarization-activated channel in interstitial cells of Cajal and gastrointestinal dysmotility induced by malignant ascites". Oncology Letters 13.3 (2017): 1601-1608.
Chicago
Guo, T., Li, J., Li, J., Kong, D., Bi, C., He, Z., Tang, D., Jin, X., Jin, L."Association between hyperpolarization-activated channel in interstitial cells of Cajal and gastrointestinal dysmotility induced by malignant ascites". Oncology Letters 13, no. 3 (2017): 1601-1608. https://doi.org/10.3892/ol.2017.5652
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