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Article Open Access

miR-141 promotes colon cancer cell proliferation by inhibiting MAP2K4

  • Authors:
    • Lei Ding
    • Li‑Li Yu
    • Ning Han
    • Bu‑Tian Zhang
  • View Affiliations / Copyright

    Affiliations: Department of Radiology, The Third Hospital of Jilin University, Changchun, Jilin 130000, P.R. China
    Copyright: © Ding et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1665-1671
    |
    Published online on: January 26, 2017
       https://doi.org/10.3892/ol.2017.5653
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Abstract

MicroRNAs (miRNAs or miRs) can function as tumor-suppressor or oncogenic genes. Upregulation of miRNA‑141 has been frequently observed in colorectal cancer (CRC) samples. The experimentally observed targets of miR‑141 include the tumor‑suppressor gene mitogen‑activated protein kinase kinase 4 (MAP2K4). The aim of the present study was to investigate the role of miR‑141 in the proliferation of colonic cancer. Western blotting, immunohistochemistry and reverse transcription‑quantitative polymerase chain reaction were used to detect the expression levels of miR‑141 and MAP2K4 in colonic adenocarcinoma (CAC) and adjacent non‑cancerous (NC) tissue samples, as well as in human CAC cell lines (HT29, T94 and LS174). MTT assay was used to investigate the proliferation and apoptosis of these three cell lines. The expression levels of miR‑141 were significantly upregulated in clinical samples of CAC, compared with adjacent NC tissues. By contrast, MAP2K4 was downregulated in CAC. The in vitro assays demonstrated that overexpression of miR‑141 resulted in cell proliferation of CAC by inhibiting MAP2K4 activity. Our study suggests that targeting the miR‑141‑MAP2K4 signaling pathway may represent a novel approach for the treatment of CRC.
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Copy and paste a formatted citation
Spandidos Publications style
Ding L, Yu LL, Han N and Zhang BT: miR-141 promotes colon cancer cell proliferation by inhibiting MAP2K4. Oncol Lett 13: 1665-1671, 2017.
APA
Ding, L., Yu, L., Han, N., & Zhang, B. (2017). miR-141 promotes colon cancer cell proliferation by inhibiting MAP2K4. Oncology Letters, 13, 1665-1671. https://doi.org/10.3892/ol.2017.5653
MLA
Ding, L., Yu, L., Han, N., Zhang, B."miR-141 promotes colon cancer cell proliferation by inhibiting MAP2K4". Oncology Letters 13.3 (2017): 1665-1671.
Chicago
Ding, L., Yu, L., Han, N., Zhang, B."miR-141 promotes colon cancer cell proliferation by inhibiting MAP2K4". Oncology Letters 13, no. 3 (2017): 1665-1671. https://doi.org/10.3892/ol.2017.5653
Copy and paste a formatted citation
x
Spandidos Publications style
Ding L, Yu LL, Han N and Zhang BT: miR-141 promotes colon cancer cell proliferation by inhibiting MAP2K4. Oncol Lett 13: 1665-1671, 2017.
APA
Ding, L., Yu, L., Han, N., & Zhang, B. (2017). miR-141 promotes colon cancer cell proliferation by inhibiting MAP2K4. Oncology Letters, 13, 1665-1671. https://doi.org/10.3892/ol.2017.5653
MLA
Ding, L., Yu, L., Han, N., Zhang, B."miR-141 promotes colon cancer cell proliferation by inhibiting MAP2K4". Oncology Letters 13.3 (2017): 1665-1671.
Chicago
Ding, L., Yu, L., Han, N., Zhang, B."miR-141 promotes colon cancer cell proliferation by inhibiting MAP2K4". Oncology Letters 13, no. 3 (2017): 1665-1671. https://doi.org/10.3892/ol.2017.5653
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