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Article Open Access

A proposed method for the relative quantification of levels of circulating microRNAs in the plasma of gastric cancer patients

Corrigendum in: /10.3892/ol.2017.7626
  • Authors:
    • Mayra‑Cecilia Suárez‑Arriaga
    • Javier Torres
    • Margarita Camorlinga‑Ponce
    • Alejandro Gómez‑Delgado
    • Patricia Piña‑Sánchez
    • Hilda‑Alicia Valdez‑Salazar
    • Rosa‑María Ribas‑Aparicio
    • Ezequiel M. Fuentes‑Pananá
    • Martha‑Eugenia Ruiz‑Tachiquín
  • View Affiliations / Copyright

    Affiliations: Medical Research Unit in Human Genetics, Pediatrics Hospital ‘Dr Silvestre Frenk Freud’, XXI Century National Medical Center, Mexican Institute of Social Security, 06720 Mexico City, Mexico, Medical Research Unit in Infectious and Parasitic Diseases, Pediatrics Hospital ‘Dr Silvestre Frenk Freud’, XXI Century National Medical Center, Mexican Institute of Social Security, 06720 Mexico City, Mexico, Medical Research Unit in Oncological Diseases, Oncology Hospital, XXI Century National Medical Center, Mexican Institute of Social Security, 06720 Mexico City, Mexico, Department of Microbiology, National School of Biological Sciences, National Polytechnic Institute, 11340 Mexico City, Mexico, Virology and Cancer Research Unit, Children's Hospital of Mexico ‘Federico Gómez’, 06720 Mexico City, Mexico
    Copyright: © Suárez‑Arriaga et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 3109-3117
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    Published online on: March 7, 2017
       https://doi.org/10.3892/ol.2017.5816
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Abstract

Gastric cancer (GC) is the fifth most common type of malignancy and the third leading cause of cancer-associated mortality worldwide. It is necessary to identify novel methods aimed at improving the early diagnosis and treatment of GC. MicroRNA expression profiles in the plasma of patients with GC have demonstrated a potential use in the opportune diagnosis of this neoplasm. However, there are currently no standardized targets for use in the normalization of microRNA Cq values for different neoplasms. The present study tested two normalization approaches while analyzing plasma derived from patients with GC and non‑atrophic gastritis. The first method utilized a panel of small nucleolar RNAs (snoRNAs) and a small nuclear RNA (snRNA) provided by a commercial array. The second normalization approach involved the use of hsa‑miR‑18a‑5p and hsa‑miR‑29a‑3p, which were identified by a stability analysis of the samples being tested. The results revealed that the snoRNAs and snRNA were not expressed in all samples tested. Only the stable microRNAs allowed a narrow distribution of the data and enabled the identification of specific downregulation of hsa‑miR‑200c‑3p and hsa‑miR‑26b‑5p in patients with GC. hsa‑miR‑200c‑3p and hsa-miR-26b-5p have been previously linked to cancer, and a Kyoto Encyclopedia of Genes and Genomes analysis demonstrated that these microRNAs were associated with cell adhesion, cell cycle and cancer pathways.
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Copy and paste a formatted citation
Spandidos Publications style
Suárez‑Arriaga MC, Torres J, Camorlinga‑Ponce M, Gómez‑Delgado A, Piña‑Sánchez P, Valdez‑Salazar HA, Ribas‑Aparicio RM, Fuentes‑Pananá EM and Ruiz‑Tachiquín ME: A proposed method for the relative quantification of levels of circulating microRNAs in the plasma of gastric cancer patients Corrigendum in /10.3892/ol.2017.7626. Oncol Lett 13: 3109-3117, 2017.
APA
Suárez‑Arriaga, M., Torres, J., Camorlinga‑Ponce, M., Gómez‑Delgado, A., Piña‑Sánchez, P., Valdez‑Salazar, H. ... Ruiz‑Tachiquín, M. (2017). A proposed method for the relative quantification of levels of circulating microRNAs in the plasma of gastric cancer patients Corrigendum in /10.3892/ol.2017.7626. Oncology Letters, 13, 3109-3117. https://doi.org/10.3892/ol.2017.5816
MLA
Suárez‑Arriaga, M., Torres, J., Camorlinga‑Ponce, M., Gómez‑Delgado, A., Piña‑Sánchez, P., Valdez‑Salazar, H., Ribas‑Aparicio, R., Fuentes‑Pananá, E. M., Ruiz‑Tachiquín, M."A proposed method for the relative quantification of levels of circulating microRNAs in the plasma of gastric cancer patients Corrigendum in /10.3892/ol.2017.7626". Oncology Letters 13.5 (2017): 3109-3117.
Chicago
Suárez‑Arriaga, M., Torres, J., Camorlinga‑Ponce, M., Gómez‑Delgado, A., Piña‑Sánchez, P., Valdez‑Salazar, H., Ribas‑Aparicio, R., Fuentes‑Pananá, E. M., Ruiz‑Tachiquín, M."A proposed method for the relative quantification of levels of circulating microRNAs in the plasma of gastric cancer patients Corrigendum in /10.3892/ol.2017.7626". Oncology Letters 13, no. 5 (2017): 3109-3117. https://doi.org/10.3892/ol.2017.5816
Copy and paste a formatted citation
x
Spandidos Publications style
Suárez‑Arriaga MC, Torres J, Camorlinga‑Ponce M, Gómez‑Delgado A, Piña‑Sánchez P, Valdez‑Salazar HA, Ribas‑Aparicio RM, Fuentes‑Pananá EM and Ruiz‑Tachiquín ME: A proposed method for the relative quantification of levels of circulating microRNAs in the plasma of gastric cancer patients Corrigendum in /10.3892/ol.2017.7626. Oncol Lett 13: 3109-3117, 2017.
APA
Suárez‑Arriaga, M., Torres, J., Camorlinga‑Ponce, M., Gómez‑Delgado, A., Piña‑Sánchez, P., Valdez‑Salazar, H. ... Ruiz‑Tachiquín, M. (2017). A proposed method for the relative quantification of levels of circulating microRNAs in the plasma of gastric cancer patients Corrigendum in /10.3892/ol.2017.7626. Oncology Letters, 13, 3109-3117. https://doi.org/10.3892/ol.2017.5816
MLA
Suárez‑Arriaga, M., Torres, J., Camorlinga‑Ponce, M., Gómez‑Delgado, A., Piña‑Sánchez, P., Valdez‑Salazar, H., Ribas‑Aparicio, R., Fuentes‑Pananá, E. M., Ruiz‑Tachiquín, M."A proposed method for the relative quantification of levels of circulating microRNAs in the plasma of gastric cancer patients Corrigendum in /10.3892/ol.2017.7626". Oncology Letters 13.5 (2017): 3109-3117.
Chicago
Suárez‑Arriaga, M., Torres, J., Camorlinga‑Ponce, M., Gómez‑Delgado, A., Piña‑Sánchez, P., Valdez‑Salazar, H., Ribas‑Aparicio, R., Fuentes‑Pananá, E. M., Ruiz‑Tachiquín, M."A proposed method for the relative quantification of levels of circulating microRNAs in the plasma of gastric cancer patients Corrigendum in /10.3892/ol.2017.7626". Oncology Letters 13, no. 5 (2017): 3109-3117. https://doi.org/10.3892/ol.2017.5816
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