Open Access

siRNA-mediated knockdown of ID1 disrupts Nanog- and Oct-4-mediated cancer stem cell-likeness and resistance to chemotherapy in gastric cancer cells

  • Authors:
    • Linlin Li
    • Xiaoyong Wei
    • Baofeng Wu
    • Yuanli Xiao
    • Mingzhu Yin
    • Qiaohong Yang
  • View Affiliations

  • Published online on: March 8, 2017     https://doi.org/10.3892/ol.2017.5828
  • Pages: 3014-3024
  • Copyright: © Li et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

DNA-binding protein inhibitor ID-1 (ID1) serves an essential role in tumor progression, and the self‑renewal and pluripotency of embryonic stem cells. However, the effect of ID1 on the stemness and cancer stem cell (CSC)‑like properties of gastric adenocarcinoma cells remains to be elucidated. In the present study, effective ID1 knockdown was achieved in gastric cancer (GC) cells using small interfering RNA, and the self‑renewal ability and cisplatin (DDP) sensitivity of GC cells was subsequently examined. ID1 knockdown in the MKN‑28 and MGC‑803 cell lines was demonstrated to significantly suppress colony formation (P=0.005 in MKN‑28 and P=0.001 in MGC‑803), tumor spheroid formation (P=0.021 in MKN‑28 and P=0.037 in MGC‑803), cell proliferation (P=0.028 in MKN‑28 and P=0.001 in MGC‑803) and migration (P=0.002 in MKN‑28 and P=0.015 in MGC‑803). To the best of our knowledge, the present study revealed for the first time that ID1 knockdown suppresses the expression of the key CSC‑associated factors Nanog and octamer‑binding protein 4 (Oct‑4). It was further demonstrated that ID1 knockdown sensitized GC cells to DDP. In conclusion, knockdown of ID1 attenuates the stem cell like‑properties of self‑renewal in normal GC cells, potentially through the targeting of Nanog and Oct‑4, and subsequently decreases cell proliferation and resistance to DDP. The results of the present study suggest that ID1 functions as an oncogene in GC and regulates the stem cell like-properties of gastric cancer cells by targeting Nanog and Oct-4.
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May-2017
Volume 13 Issue 5

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Li L, Wei X, Wu B, Xiao Y, Yin M and Yang Q: siRNA-mediated knockdown of ID1 disrupts Nanog- and Oct-4-mediated cancer stem cell-likeness and resistance to chemotherapy in gastric cancer cells. Oncol Lett 13: 3014-3024, 2017
APA
Li, L., Wei, X., Wu, B., Xiao, Y., Yin, M., & Yang, Q. (2017). siRNA-mediated knockdown of ID1 disrupts Nanog- and Oct-4-mediated cancer stem cell-likeness and resistance to chemotherapy in gastric cancer cells. Oncology Letters, 13, 3014-3024. https://doi.org/10.3892/ol.2017.5828
MLA
Li, L., Wei, X., Wu, B., Xiao, Y., Yin, M., Yang, Q."siRNA-mediated knockdown of ID1 disrupts Nanog- and Oct-4-mediated cancer stem cell-likeness and resistance to chemotherapy in gastric cancer cells". Oncology Letters 13.5 (2017): 3014-3024.
Chicago
Li, L., Wei, X., Wu, B., Xiao, Y., Yin, M., Yang, Q."siRNA-mediated knockdown of ID1 disrupts Nanog- and Oct-4-mediated cancer stem cell-likeness and resistance to chemotherapy in gastric cancer cells". Oncology Letters 13, no. 5 (2017): 3014-3024. https://doi.org/10.3892/ol.2017.5828