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Article

Long non‑coding RNA GAS5 inhibits cell proliferation, induces G0/G1 arrest and apoptosis, and functions as a prognostic marker in colorectal cancer

  • Authors:
    • Yang Yang
    • Zhanlong Shen
    • Yichao Yan
    • Bo Wang
    • Jizhun Zhang
    • Chao Shen
    • Tao Li
    • Chunxiang Ye
    • Zhidong Gao
    • Guo Peng
    • Yingjiang Ye
    • Kewei Jiang
    • Shan Wang
  • View Affiliations / Copyright

    Affiliations: Department of Gastroenterological Surgery, Peking University People's Hospital, Beijing 100034, P.R. China
  • Pages: 3151-3158
    |
    Published online on: March 10, 2017
       https://doi.org/10.3892/ol.2017.5841
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Abstract

Colorectal cancer (CRC) is the third most common cancer worldwide and its treatment remains a challenge. Effective control of cell survival and proliferation is critical in the prevention of oncogenesis and successful treatment of cancer. Long non‑coding RNAs (lncRNAs) have emerged as primary regulators of carcinogenesis. Growth arrest specific 5 (GAS5), a lncRNA, is known to be aberrantly expressed in several types of cancer, however, the role of GAS5 in CRC remains unclear. In the present study, GAS5 mRNA expression was measured in CRC and adjacent normal mucosa tissue samples from 53 patients using reverse transcription‑quantitative polymerase chain reaction analysis, in addition to seven CRC cell lines. GAS5 mRNA expression was observed to be markedly downregulated in human CRC tissues and cell lines. Decreased GAS5 expression was associated with an increase in tumor diameter [odds ratio (OR), 0.176 (95% CI, 0.053‑0.586); P=0.003] and later tumor‑node‑metastasis stage [OR, 0.261 (95% CI, 0.083‑0.819); P=0.019]. Patients with decreased GAS5 expression exhibited decreased overall survival rates compared with patients with increased GAS5 expression (P=0.015). The Cox proportional hazards model demonstrated that downregulated GAS5 expression was an independent prognostic factor for CRC (hazard ratio, 0.236; 95% confidence interval, 0.067‑0.827; P=0.024). Functional assays demonstrated that overexpression of GAS5 inhibited cell proliferation and survival, and induced G0/G1 cell cycle arrest and apoptosis; however, knockdown of GAS5 expression enhanced cell proliferation, and reduced G0/G1 arrest and apoptosis. In conclusion, the results of the present study suggest that GAS5 is essential in the control of apoptosis and cell growth in CRC. Therefore, GAS5 may represent a novel prognostic and diagnostic marker of CRC, in addition to being a potential therapeutic target.
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Copy and paste a formatted citation
Spandidos Publications style
Yang Y, Shen Z, Yan Y, Wang B, Zhang J, Shen C, Li T, Ye C, Gao Z, Peng G, Peng G, et al: Long non‑coding RNA GAS5 inhibits cell proliferation, induces G0/G1 arrest and apoptosis, and functions as a prognostic marker in colorectal cancer. Oncol Lett 13: 3151-3158, 2017.
APA
Yang, Y., Shen, Z., Yan, Y., Wang, B., Zhang, J., Shen, C. ... Wang, S. (2017). Long non‑coding RNA GAS5 inhibits cell proliferation, induces G0/G1 arrest and apoptosis, and functions as a prognostic marker in colorectal cancer. Oncology Letters, 13, 3151-3158. https://doi.org/10.3892/ol.2017.5841
MLA
Yang, Y., Shen, Z., Yan, Y., Wang, B., Zhang, J., Shen, C., Li, T., Ye, C., Gao, Z., Peng, G., Ye, Y., Jiang, K., Wang, S."Long non‑coding RNA GAS5 inhibits cell proliferation, induces G0/G1 arrest and apoptosis, and functions as a prognostic marker in colorectal cancer". Oncology Letters 13.5 (2017): 3151-3158.
Chicago
Yang, Y., Shen, Z., Yan, Y., Wang, B., Zhang, J., Shen, C., Li, T., Ye, C., Gao, Z., Peng, G., Ye, Y., Jiang, K., Wang, S."Long non‑coding RNA GAS5 inhibits cell proliferation, induces G0/G1 arrest and apoptosis, and functions as a prognostic marker in colorectal cancer". Oncology Letters 13, no. 5 (2017): 3151-3158. https://doi.org/10.3892/ol.2017.5841
Copy and paste a formatted citation
x
Spandidos Publications style
Yang Y, Shen Z, Yan Y, Wang B, Zhang J, Shen C, Li T, Ye C, Gao Z, Peng G, Peng G, et al: Long non‑coding RNA GAS5 inhibits cell proliferation, induces G0/G1 arrest and apoptosis, and functions as a prognostic marker in colorectal cancer. Oncol Lett 13: 3151-3158, 2017.
APA
Yang, Y., Shen, Z., Yan, Y., Wang, B., Zhang, J., Shen, C. ... Wang, S. (2017). Long non‑coding RNA GAS5 inhibits cell proliferation, induces G0/G1 arrest and apoptosis, and functions as a prognostic marker in colorectal cancer. Oncology Letters, 13, 3151-3158. https://doi.org/10.3892/ol.2017.5841
MLA
Yang, Y., Shen, Z., Yan, Y., Wang, B., Zhang, J., Shen, C., Li, T., Ye, C., Gao, Z., Peng, G., Ye, Y., Jiang, K., Wang, S."Long non‑coding RNA GAS5 inhibits cell proliferation, induces G0/G1 arrest and apoptosis, and functions as a prognostic marker in colorectal cancer". Oncology Letters 13.5 (2017): 3151-3158.
Chicago
Yang, Y., Shen, Z., Yan, Y., Wang, B., Zhang, J., Shen, C., Li, T., Ye, C., Gao, Z., Peng, G., Ye, Y., Jiang, K., Wang, S."Long non‑coding RNA GAS5 inhibits cell proliferation, induces G0/G1 arrest and apoptosis, and functions as a prognostic marker in colorectal cancer". Oncology Letters 13, no. 5 (2017): 3151-3158. https://doi.org/10.3892/ol.2017.5841
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