Effect of SATB1 silencing on the proliferation, invasion and apoptosis of TE-1 esophageal cancer cells

Huang,Bo; Xiong,Fei; Wang,Siwang; Lang,Xianping; Wang,Xiaodong; Zhou,Hongli

doi:10.3892/ol.2017.5854

Effect of SATB1 silencing on the proliferation, invasion and apoptosis of TE-1 esophageal cancer cells

Authors:
- Bo Huang
- Fei Xiong
- Siwang Wang
- Xianping Lang
- Xiaodong Wang
- Hongli Zhou
View Affiliations

Affiliations: Department of Thoracic Surgery, The First Affiliated Hospital of Liaoning Medical University, Jinzhou, Liaoning 121000, P.R. China, Department of Nephropathy, The First Affiliated Hospital of Liaoning Medical University, Jinzhou, Liaoning 121000, P.R. China
Published online on: March 13, 2017 https://doi.org/10.3892/ol.2017.5854
Pages: 2915-2920
Copyright: © Huang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )

Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )

Cited By (CrossRef): 0 citations Loading Articles...

Abstract

The aim of the present study was to investigate the effect of special AT-rich sequence-binding protein-1 (SATB1)-targeted small interfering RNA (siRNA) on the proliferation, invasion and apoptosis of TE‑1 human esophageal cancer cells. SATB1 has been correlated with the metastasis and poor prognosis of colon and breast cancer, but the role of SATB1 in esophageal cancer remains unknown. Therefore, the present study constructed and transfected SATB1‑siRNA into TE‑1 cells in order to knockdown the expression of the SATB1 gene. Western blot analysis, a cell counting kit, transwell chamber assays and flow cytometry were used to assess the effect of SATB1‑siRNA on the proliferation, invasion and apoptosis of cells. The results demonstrated that the expression of the SATB1 gene was efficiently knocked down by SATB1‑siRNA, and that SATB1-siRNA inhibited the proliferation, invasion and apoptosis of TE‑1 cells. Therefore, it was concluded that the SATB1 gene is important in the pathogenesis of human esophageal cancer, and may present a novel therapeutic target for esophageal cancer.

View Figures

View References

1	Chuang SC, Hashibe M, Scelo G, Brewster DH, Pukkala E, Friis S, Tracey E, Weiderpass E, Hemminki K, Tamaro S, et al: Risk of second primary cancer among esophageal cancer patients: A pooled analysis of 13 cancer registries. Cancer Epidemiol Biomarkers Prev. 17:1543–1549. 2008. View Article : Google Scholar : PubMed/NCBI
2	Reddy Lakshminarayana CN, Vyjayanti VN, Notani D, Galande S and Kotamraju S: Down-regulation of the global regulator SATB1 by statins in COLO205 colon cancer cells. Mol Med Rep. 3:857–861. 2010.PubMed/NCBI
3	Ordinario E, Han HJ, Furuta S, Heiser LM, Jakkula LR, Rodier F, Spellman PT, Campisi J, Gray JW, Bissell MJ, et al: ATM suppresses SATB1-induced malignant progression in breast epithelial cells. PLoS One. 7:e517862012. View Article : Google Scholar : PubMed/NCBI
4	Al-Sohaily S, Henderson C, Selinger C, Pangon L, Segelov E, Kohonen-Corish MR and Warusavitarne J: Loss of special AT-rich sequence-binding protein 1 (SATB1) predicts poor survival in patients with colorectal cancer. Histopathology. 65:155–163. 2014. View Article : Google Scholar : PubMed/NCBI
5	Nakayama Y, Mian IS, Kohwi-Shigematsu T and Ogawa T: A nuclear targeting determinant for SATB1, a genome organizer in the T cell lineage. Cell Cycle. 4:1099–1106. 2005. View Article : Google Scholar : PubMed/NCBI
6	Cai S, Han HJ and Kohwi-Shigematsu T: Tissue-specific nuclear architecture and gene expression regulated by SATB1. Nat Genet. 34:42–51. 2003. View Article : Google Scholar : PubMed/NCBI
7	Frömberg A, Rabe M and Aigner A: Multiple effects of the special AT-rich binding protein 1 (SATB1) in colon carcinoma. Int J Cancer. 135:2537–2546. 2014. View Article : Google Scholar : PubMed/NCBI
8	Wang Y, Gu X, Zhang G, Wang L, Wang T, Zhao Y, Zhang X, Zhou Y, Kadin M and Tu P: SATB1 overexpression promotes malignant T-cell proliferation in cutaneous CD30+ lymphoproliferative disease by repressing p21. Blood. 123:3452–3461. 2014. View Article : Google Scholar : PubMed/NCBI
9	Grzanka J, Leveson-Gower D, Golab K, Wang XJ, Marek-Trzonkowska N, Krzystyniak A, Wardowska A, Mills JM, Trzonkowski P and Witkowski P: FoxP3, Helios, and SATB1: Roles and relationships in regulatory T cells. Int Immunopharmacol. 16:343–347. 2013. View Article : Google Scholar : PubMed/NCBI
10	Kobierzycki C, Wojnar A and Dziegiel P: Expression of SATB1 protein in the ductal breast carcinoma tissue microarrays-preliminary study. Folia Histochem Cytobiol. 51:333–338. 2013. View Article : Google Scholar : PubMed/NCBI
11	Kim CJ, Lee GR, Shin JW, Jung SW, Park BR and Park NH: Mutational analysis of SATB1 gene in hepatocellular carcinomas. APMIS. 121:1012–1014. 2013. View Article : Google Scholar : PubMed/NCBI
12	Tracz AF, Peczek Ł, Zuk K, Stec-Michalska K and Nawrot B: Effect of Helicobacter pylori eradication on the expression level of SATB1 and c-Myc genes in gastric mucosa of patients with family history of gastric cancer. Pol Merkur Lekarski. 34:269–276. 2013.(In Polish). PubMed/NCBI
13	Kohwi-Shigematsu T, Poterlowicz K, Ordinario E, Han HJ, Botchkarev VA and Kohwi Y: Genome organizing function of SATB1 in tumor progression. Semin Cancer Biol. 23:72–79. 2013. View Article : Google Scholar : PubMed/NCBI
14	Zhang HZ, Jin GF and Shen HB: Epidemiologic differences in esophageal cancer between Asian and Western populations. Chin J Cancer. 31:281–286. 2012. View Article : Google Scholar : PubMed/NCBI
15	Zheng S, Vuitton L, Sheyhidin I, Vuitton DA, Zhang Y and Lu X: Northwestern China: A place to learn more on oesophageal cancer. Part one: Behavioural and environmental risk factors. Eur J Gastroenterol Hepatol. 22:917–925. 2010. View Article : Google Scholar : PubMed/NCBI
16	Ferri F: ‘Esophageal Tumors’Ferri's clinical advisor 2013. Philadelphia, PA: Mosby (Elsevier); pp. 389–391. 2012
17	Hanna A, Birla R, Iosif C, Boeriu M, Tomsa R, Puscasu A and Constantinoiu S: Evaluation of neoadjuvant radiochemotherapy response (RCT) in squamous esophageal cancer (ESC) and implications in therapeutic conduct. Chirurgia (Bucur). 110:214–223. 2015.PubMed/NCBI
18	Berry MF: Esophageal cancer: Staging system and guidelines for staging and treatment. J Thorac Dis. 6:(Suppl 3). S289–S297. 2014.PubMed/NCBI
19	Soni B, el Hassan B and Mahmoud B: Chemical isolation and characterization of different cellulose nanofibers from cotton stalks. Carbohydr Polym. 134:581–589. 2015. View Article : Google Scholar : PubMed/NCBI
20	Kumar Pavan P, Purbey PK, Sinha CK, Notani D, Limaye A, Jayani RS and Galande S: Phosphorylation of SATB1, a global gene regulator, acts as a molecular switch regulating its transcriptional activity in vivo. Mol Cell. 22:231–243. 2006. View Article : Google Scholar : PubMed/NCBI
21	Hsu SM and Jaffe ES: Phenotypic expression of B-lymphocytes. 1. Identification with monoclonal antibodies in normal lymphoid tissues. Am J Pathol. 114:387–395. 1984.PubMed/NCBI
22	Gudat FG, Forster HK, Suter F, Albrecht R, Krey G, Dürmüller U, Girard MF and Obrecht JP: Tissue distribution and ultrastructure of B lymphocytes reacting with the monoclonal antibody anti-Y29/55. Virchows Arch B Cell Pathol Incl Mol Pathol. 48:341–350. 1985. View Article : Google Scholar : PubMed/NCBI
23	Han HJ, Russo J, Kohwi Y and Kohwi-Shigematsu T: SATB1 reprogrammes gene expression to promote breast tumour growth and metastasis. Nature. 452:187–193. 2008. View Article : Google Scholar : PubMed/NCBI
24	Peng Z, Wang C, Fang E, Lu X, Wang G and Tong Q: Co-delivery of doxorubicin and SATB1 shRNA by thermosensitive magnetic cationic liposomes for gastric cancer therapy. PLoS One. 9:e929242014. View Article : Google Scholar : PubMed/NCBI
25	Fang XF, Hou ZB, Dai XZ, Chen C, Ge J, Shen H, Li XF, Yu LK and Yuan Y: Special AT-rich sequence-binding protein 1 promotes cell growth and metastasis in colorectal cancer. World J Gastroenterol. 19:2331–2339. 2013. View Article : Google Scholar : PubMed/NCBI
26	Huang B, Zhou H, Wang X and Liu Z: Silencing SATB1 with siRNA inhibits the proliferation and invasion of small cell lung cancer cells. Cancer Cell Int. 13:82013. View Article : Google Scholar : PubMed/NCBI
27	Zhou L, Yu L, Zhu B, Wu S, Song W, Gong X and Wang D: Metastasis-associated in colon cancer-1 and aldehyde dehydrogenase 1 are metastatic and prognostic biomarker for non-small cell lung cancer. BMC Cancer. 16:8762016. View Article : Google Scholar : PubMed/NCBI
28	Xiang J, Zhou L, Li S, Xi X, Zhang J, Wang Y, Yang Y, Liu X and Wan X: AT-rich sequence binding protein 1: Contribution to tumor progression and metastasis of human ovarian carcinoma. Oncol Lett. 3:865–870. 2012.PubMed/NCBI
29	Zheng J: Is SATB1 a master regulator in breast cancer growth and metastasis? Womens Health (Lond). 4:329–332. 2008. View Article : Google Scholar : PubMed/NCBI