An Ω-3 fatty acid desaturase-expressing gene attenuates prostate cancer proliferation by cell cycle regulation

Introduction

Prostate cancer is the fourth leading cause of cancer-associated mortality and the second most prevalent cancer worldwide (1). In developed countries, prostate cancer is the most commonly diagnosed cancer in men and affects >17% of men worldwide (1). In the US, prostate cancer is the most common malignancy and the second leading cause of cancer-associated mortality (2). Globally, a high incidence of prostate cancer is associated with excessive consumption of Ω-6 polyunsaturated fatty acids (PUFAs), commonly found in red and organ meats, refined vegetable oils and cooked processed meat (3–5). Conversely, evidence suggests that an Ω-3 PUFA-rich diet is inversely associated with prostate cancer development (6–9).

Both Ω-6 and Ω-3 PUFAs are essential fatty acids (10), and while Ω-3 PUFAs have protective effects, Ω-6 PUFAs may serve a role in cancer development (11–14). It has been demonstrated that dietary intake of long-chain Ω-3 PUFAs reduces the incidence of several types of cancer and alleviates cancer-associated complications (15,16). Clinically, long-term intake of dietary or supplemental docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA) (both Ω-3 PUFAs) is associated with a decreased risk of prostate cancer development (6–9), increased sensitivity to chemotherapy treatment (14) and decreased risk of advanced stage disease, metastases and cancer-associated mortality (8,17,18). Additionally, dietary Ω-3 PUFAs enhance hormone ablation therapy in androgen-dependent prostate cancer (19) and attenuate prostate cancer growth in primary prostate cancer development (20) and xenograft models (19). Therefore, Ω-3 PUFAs may be important in the prevention and treatment of prostate cancer.

The EPA-rich Saprolegnia diclina gene, sdd17, encodes an Ω-3 fatty acid desaturase that converts exogenous arachidonic acid (AA), an Ω-6 PUFA, into EPA (21). It has been reported that sdd17 from EPA-rich fungus is expressed at high levels and increases Ω-3 fatty acid concentrations in mammalian cells (22). In the present study, prostate cancer cells were infected with a lentivirus carrying the sdd17 gene and the underlying mechanisms of Ω-3 PUFAs on prostate cell proliferation were evaluated. The potential positive outcomes of the present study may benefit patients with prostate cancer.

Materials and methods

Reagents

All cell culture reagents were purchased from Incoterm Fisher Scientific, Inc. (Waltham, MA, USA). AA, EPA, propidium iodide (PI) and PUFA standards were obtained from Sigma-Aldrich; Merck Millipore (Darmstadt, Germany). Water-soluble tetrazolium (WST) was acquired from Dojindo Molecular Technologies, Inc. (Kumamoto, Japan). Anti-E2F transcription factor 1 antibodies (#sc-251; dilution, 1:1,000) were obtained from Santa Cruz Biotechnology, Inc. (Dallas, TX, USA) and anti-β-actin antibodies (#ab8226; dilution, 1:2,000) were purchased from Abcam (Cambridge, UK).

Cell culture and proliferation assays

The human prostate cancer cell line VCaP was obtained from the American Type Culture Collection (Manassas, VA, USA). VCaP cells were cultured at 37°C and 5% CO2 in Dulbecco's modified Eagle's medium supplemented with 10% fetal bovine serum (Invitrogen; Thermo Fisher Scientific, Inc.), 100 IU/ml penicillin and 100 µg/ml streptomycin. Cell proliferation assays were performed in 96-well plates. Cells (5×103 per well) were incubated for 24 h with different concentrations of PUFAs (10–50 µM), stained with WST at 37°C for 1 h and quantified at 450 nm (ELX800; BioTek Instruments, Inc., Winooski, VT, USA).

Overexpression of msdd17 by lentiviral transfection

The sdd17 gene was cloned from S. diclina based on its nucleotide sequence (GenBank accession no. AY373823). The codons of sdd17 cDNA were optimized for efficient translation in mammalian cells, resulting in the msdd17 gene. The msdd17 cDNA was subsequently inserted into the PLJM1 lentivirus vector (Addgene, Inc., Cambridge, MA, USA); pMD2.G and psPAX2 plasmids were co-transfected with PLJM1-msdd17 (vehicle plasmid) into 293T cells (American Type Culture Collection, Manassas, VA, USA) using X-tremeGENE™ HP DNA transfection reagent (Roche Diagnostics, Indianapolis, IN, USA). In order to generate a stable msdd17-overexpressing cell line, lentivirus-containing supernatant was harvested 48 h post-transfection and used to infect VCaP cells.

Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) analysis of msdd17 gene expression levels

Total RNA was extracted using TRIzol® reagent, and was subsequently treated with DNase I and reverse transcribed using the PrimeScript™ RT reagent kit (Takara Bio, Inc., Shiga, Japan) following the manufacturer protocol. qPCR was performed using an Applied Biosystems StepOnePlus™ Real-Time PCR system (Applied Biosystems; Thermo Fisher Scientific, Inc.). FastStart Universal SYBR Green Master (Rox) was obtained from Roche Diagnostics (#04913914001). The qPCR conditions consisted of 1 cycle at 50°C for 2 min, followed by 1 cycle at 95°C for 30 sec, and 40 cycles at 95°C for 15 sec, 58°C for 30 sec and 72°C for 30 sec. The experiments were repeated six times. The results were normalized according to the expression levels of β-actin RNA. Results were expressed using the 2−ΔΔCq method (23). The primer sequences were as follows: msdd17, forward, 5′-GTACACAAACCAAGCTCCGC-3′ and reverse, 5′-CCATCCTGACCCCATTCGAG-3′; and β-actin, forward, 5′-GCTCTGGCTCCTAGCACCAT-3′ and reverse, 5′-GGGCCGGACTCATCGTACT-3′.

Gas chromatography (GC) analysis

Lipid extraction from VCaP cells was performed following a previously described protocol (24). Gas chromatography was performed on an Agilent 7890A Gas Chromatograph (Agilent Technologies, Inc., Santa Clara, CA, USA). Compounds were identified by comparing their retention times with those of PUFA standards.

Cell cycle analysis

Following incubation overnight in Dulbecco's modified Eagle's medium (Invitrogen), VCaP cells were harvested and rinsed with PBS twice. Prior to cell cycle analysis, cells were fixed in 70% pre-cold ethanol at 4°C overnight, and prior to flow cytometry, cells were washed twice with PBS and then resuspended with PBS containing 10 mg/ml PI (Sigma-Aldrich; Merck Millipore) and incubated for 15 min in the dark at room temperature. DNA content was analyzed using a BD FACStar flow cytometer and the percentages of different cell cycle phases were determined using a ModFit LT software version 4.0 (BD Biosciences, San Jose, CA, USA).

Western blotting

VCaP cells were treated with PUFA agents at 37°C for various times as indicated in the experiments. The cells were collected into lysis buffer [50 mM Tris-HCl (pH 8.0), 150 mM NaCl, 0.5% sodium deoxycholate, 1% Nonidet P-40, 0.1% SDS, 100 g/ml PMSF and aprotinin] and placed on ice for 30 min. Cell lysates were sonicated on ice for at least 30 sec and then cleared by centrifugation at 12,000 × g for 30 min at 4°C. Equal amounts of total protein (40 µg) were separated by SDS-PAGE and transferred onto a nitrocellulose membrane. The membranes were probed with the appropriate antibodies at 4°C overnight with gentle agitation. Immunoreactivity was detected by ECL and quantified using ImageLab version 4.0 analysis software (Bio-Rad Laboratories, Inc., Hercules, CA, USA).

Statistical analysis

Data are presented as the mean ± standard error of at least three independent experiments. All statistical analyses were performed using GraphPad Prism version 5.01 (GraphPad Software, Inc., La Jolla, CA, USA) and included either two-tailed Student's t-test or one-way analysis of variance followed by Dunnett's test for comparing the means of two or multiple groups, respectively. P<0.05 was considered to indicate a statistically significant difference.

Results

Effect of Ω-3 PUFAs on VCaP cell growth

To assess the effects of Ω-3 PUFAs on prostate cancer cells, VCaP cells were exposed to either AA or EPA. The results demonstrated that AA stimulated VCaP cell proliferation (Fig. 1A), which was consistent with previous observations in breast (24) and endometrial (25,26) cancer cell lines. Conversely, EPA effectively inhibited prostate cancer cell growth in vitro. At the highest concentration (50 µM), EPA caused an 8-fold reduction in VCaP cell viability (Fig. 1B), demonstrating that it inhibited VCaP cell viability in a dose-dependent manner.

Figure 1. Effect of exogenous Ω-3 polyunsaturated fatty acids on prostate cancer cell growth in vitro. VCaP cells grown in 96-well plates were treated with varying concentrations of (A) AA or (B) EPA. Data were analyzed by one-way analysis of variance followed by Dunnett's test. Increasing concentrations of AA increased VCaP cell growth, while increasing concentrations of EPA decreased cell growth. The linear trend was significant in each experiment. Data are presented as the mean ± standard error of the mean (n=6). **P<0.01 vs. control cells. AA, arachidonic acid; EPA, eicosapentaenoic acid.

Effect of msdd17 on VCaP cell growth

To assess the effect of msdd17 on prostate cancer cells, VCaP cells were infected with a lentivirus carrying the msdd17 gene (VCaP-msdd17 cells). A green fluorescent protein (GFP)-expressing line, VCaP-GFP, was generated as a control.

The lentivirus carrying the msdd17 gene was used to infect VCaP cells and the co-expression of GFP allowed the identification of the cells that expressed the msdd17 gene. Following infection with the lentivirus, the VCaP cells exhibited bright fluorescence indicating a high expression level of the transgene (Fig. 2). qPCR was performed to analyze msdd17 gene expression levels. The results indicated significantly increased msdd17 expression levels in the VCaP-msdd17 cells compared with those of the control cells (Fig. 3A), demonstrating that the msdd17 gene may be highly expressed in transfected VCaP cells, which lack the gene naturally.

Figure 2. Micrographs of VCaP cells expressing GFP. Human VCaP cells were infected with a lentivirus carrying the msdd17 gene (right) or control lentivirus (left). Green fluorescence was visualized with bright light (upper) and blue light (lower). Visualization of GFP co-expression demonstrates that the msdd17 gene is highly expressed in infected cells. Scale bars, 100 µm. GFP, green fluorescent protein.

Figure 3. Effect of endogenously produced eicosapentaenoic acid on prostate cancer cell growth in vitro. (A) msdd17 gene expression in VCaP-msdd17 cells compared with controls, based on reverse transcription-quantitative polymerase chain reaction analyses. (B) VCaP-GFP and VCaP-msdd17 cells were subjected to cell growth assays. The msdd17 gene significantly inhibited cell viability. **P<0.01 vs. control cells. Data are presented as the mean ± standard error of the mean (n=6). GFP, green fluorescent protein.

Msdd17 gene transfection resulted in the conversion of Ω-6 PUFAs into Ω-3 PUFAs in the VCaP cells (Table I). GC analyses demonstrated that 26% of AA was converted into EPA (Table I). Consistent with this observation, msdd17 gene expression significantly suppressed VCaP cell viability (Fig. 3B). Taken together, these results indicated that endogenous EPA, mediated by msdd17 gene expression, directly inhibited prostate cancer cell growth.

Table I. Ω-6 and Ω-3 PUFA levels in VCaP-msdd17 cells compared with controls.

Table I.

Ω-6 and Ω-3 PUFA levels in VCaP-msdd17 cells compared with controls.

PUFAs, %	VCaP-GFP	VCaP-msdd17
LA (C18:2, Ω-6)	1.21±0.06	1.23±0.30
AA (C20:4, Ω-6)	6.21±0.12	4.57±0.07a
ALA (C18:3 Ω-3)	0.16±0.00	0.15±0.02
EPA (C20:5, Ω-3)	1.20±0.02	1.64±0.02a
DPA (C22:5, Ω-3)	2.97±0.12	4.63±0.05a
DHA (C22:6, Ω-3)	6.67±0.55	7.15±0.09

a P<0.01. Ω-6 and Ω-3 PUFA levels were determined using standard protocols (see Materials and methods). Ω-6 and Ω-3 PUFAs are expressed as a percentage of all fatty acid peaks, i.e. the distribution areas of different Ω-3 or Ω-6 PUFAs peaks divided by the total peak areas of all detectable saturated and unsaturated free fatty acids (from the same sample) obtained from gas chromatography analyses. Data are presented as the mean ± standard error of the mean (n=6). PUFA, polyunsaturated fatty acids; GFP, green fluorescent protein; LA, linoleic acid; AA, arachidonic acid; LA, α-lipoic acid; EPA, eicosapentaenoic acid; PA, docosapentaenoic acid; DHA, docosahexaenoic acid.

Effect of msdd17 on VCaP cell cycle

Flow cytometry analyses demonstrated that msdd17 gene expression resulted in G2 arrest in the cell cycle of VCaP cells (Table II). To assess the mechanism by which Ω-3 PUFAs suppress cell proliferation through cell cycle arrest, the expression of E2F1 was evaluated following EPA treatment. Western blot analysis demonstrated that EPA treatment significantly decreased E2F1 expression and that the expression of msdd17 mimicked the effect of EPA on E2F1 regulation (Fig. 4).

Figure 4. Exogenously and endogenously produced EPA induced E2F1-mediated prostate cancer cell G2 arrest in vitro. (A) E2F1 was detected by western blotting. VCaP, VCaP-GFP and VcaP-msdd17 cells were cultured without serum for 12 h and exposed to 50 µM EPA for 24 h. **P<0.01 vs. VCaP cells. (B) Data are presented as the mean ± standard error of the mean (n=3). EPA, eicosapentaenoic acid; E2F1, E2 transcription factor 1; GFP, green fluorescence protein.

Table II. Flow cytometry analyses of cell cycle in VCaP cells.

Table II.

Flow cytometry analyses of cell cycle in VCaP cells.

	G1/G0, %	S, %	G2/M, %
VCaP-GFP	80.96±1.02	10.12±0.90	8.92±0.31
VCaP-msdd17	81.22±3.35	3.40±1.83a	15.38±1.53a
EPA	78.98±2.62	3.81±1.18a	17.21±1.47a

a P<0.05. VCaP cells grown in 6-well plates were harvested for cell cycle analyses. Data are presented as the mean ± standard error of the mean (n=4). EPA, eicosapentaenoic acid; GFP, green fluorescent protein.

Discussion

It is well known that Ω-3 PUFAs have tumor-suppressing effects. The msdd17 gene, which encodes an Ω-3 fatty acid desaturase, converts AA into EPA (21,22). However, little is known regarding the function of the msdd17 gene in tumor cells in vitro. Therefore, the present study aimed to investigate the effect and underlying mechanisms of the msdd17 gene on prostate cancer. The msdd17 gene was transfected directly into VCaP cells and the inhibitory effects of the gene on prostate cancer cell proliferation was confirmed. Further experiments demonstrated that msdd17 gene expression induced G2 cell cycle arrest in prostate cancer cells and that E2F1 may be associated with this process. To the best of our knowledge, these results demonstrate, for the first time, that msdd17 gene transfection into prostate cancer cells may be used as a novel therapeutic strategy to treat prostate cancer.

Mounting evidence has linked the dietary consumption of Ω-3 PUFAs with the prevention or attenuation of several types of cancer, including breast (27), endometrial (28) and prostate (6–9) cancer. In the present study, VCaP cells were exposed to either AA or EPA, and it was demonstrated that while EPA inhibited prostate cancer cell proliferation in a dose-dependent manner, AA stimulated cell proliferation. GC analyses indicated an association between msdd17 gene expression and the successful conversion of AA into EPA. Additionally, cell proliferation assays demonstrated that msdd17 gene expression inhibited the proliferation of prostate cancer cells. Therefore, expression of the msdd17 gene may suppress tumorigenesis by simultaneously increasing endogenous EPA levels and decreasing endogenous AA levels.

E2F1 is a transcription factor involved in the pRb/E2F1 pathway and in cell cycle regulation (29), and it enhances glycolysis by suppressing Sirt6 transcription in prostate cancer cells (30). Previous studies have reported that G2/M cell cycle arrest is associated with the downregulation of E2F1 (31–33). In the present study, cell cycle analyses indicated that EPA and msdd17 gene expression inhibited prostate cancer cell proliferation by inducing prostate cancer G2 cell cycle arrest. As E2F1 serves a critical role in cellular proliferation, differentiation and apoptosis (34,35), the present study investigated whether E2F1 was involved in msdd17 gene expression-induced cell proliferation arrest in prostate cancer cells. The results demonstrated that msdd17 gene expression and exogenous EPA treatment significantly decreased E2F1 expression.

In conclusion, to the best of our knowledge, the present study is the first to evaluate the function of the msdd17 gene in tumor cells in vitro. The msdd17 gene inhibited prostate cancer cell proliferation by regulating the prostate cancer cell cycle. Therefore, stimulating the conversion of AA into EPA may be an effective therapeutic approach to treat prostate cancer.

Acknowledgements

The present study was supported by grants from the National Basic Research Program of China (973 Program; grant no. 2013CB945202), the National Natural Science Foundation of China (grant nos. 81630021 and 81170780 to A.Z., 81372798 to F.L. and 81200570 to L.X.), the Ph.D. Programs Foundation of Ministry of Education of China (grant no. 20113234110005), the Scientific Support Program of Jiangsu Province (grant no. BE2012756), the Natural Science Foundation of Jiangsu Province of China (grant nos. BK20130059 and 2011766), the Natural Science Foundation of Nanjing Medical University (grant no. 2016NJMUZD027 to J.P.), the Young Medical Talents of Jiangsu Province (grant no. QNRC2016663 to J.P.), and the High-level Innovative Talents Reward from Jiangsu Province (to F.L.).

References