An active molecule from Pulsatilla chinensis, Pulsatilla saponin A, induces apoptosis and inhibits tumor growth of human colon cancer cells without or with 5-FU
- Authors:
- Liming Xu
- Guilian Cheng
- Yi Lu
- Shaofeng Wang
View Affiliations
Affiliations: Department of Gastroenterology, The Second Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215000, P.R. China, Department of General Surgery, Suzhou Kowloon Hospital, Suzhou, Jiangsu 215021, P.R. China
- Published online on: March 21, 2017 https://doi.org/10.3892/ol.2017.5884
-
Pages:
3799-3802
Metrics: Total
Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
This article is mentioned in:
Abstract
Colon cancer is one of the common types of digestive malignancy. The efficacy of the first‑line chemotherapy drug for colon cancer, fluorouracil (5‑FU), remains limited in clinical settings due to poor efficacy and significant side effects. In the present study, the anticancer activity of an active compound from Pulsatilla chinensis extracts, Pulsatilla saponin A (PsA), was isolated and examined in vitro and in vivo. It was demonstrated that PsA significantly inhibited the growth of human colon cancer HT‑29 cells. This inhibitory activity was also observed when the compound was tested in a colon cancer xenograft mouse model. Additionally, the synergic antitumor effects of PsA and 5‑FU on colon cancer cells were observed. Using annexin V and terminal deoxynucleotidyl transferase 2'‑deoxyuridine 5'-triphosphate nick end labeling assays, it was demonstrated that levels of apoptosis induction in HT-29 cells treated with PsA or 5‑FU were significantly increased compared with the untreated control cells (P<0.05). Western blot analyses were then performed, and the results revealed an increase in tumor protein 53 and cleaved caspase 9, and a decrease in B-cell lymphoma 2 protein expressions in PsA and PsA + 5‑FU treated colon cancer cells compared with the vehicle‑treated (PBS) cells. In summary, PsA exhibited anticancer activity in human colon cancer cells in vitro and in vivo, in isolation and synergistically with 5-FU, through apoptosis induction.
View References
1
|
Cassidy J, Saltz L, Twelves C, Van Cutsem
E, Hoff P, Kang Y, Saini JP, Gilberg F and Cunningham D: Efficacy
of capecitabine versus 5-fluorouracil in colorectal and gastric
cancers: A meta-analysis of individual data from 6171 patients. Ann
Oncol. 22:2604–2609. 2011. View Article : Google Scholar : PubMed/NCBI
|
2
|
Kodama Y, Fumoto S, Nishi J, Nakashima M,
Sasaki H, Nakamura J and Nishida K: Absorption and distribution
characteristics of 5-fluorouracil (5-FU) after an application to
the liver surface in rats in order to reduce systemic side effects.
Biol Pharm Bull. 31:1049–1052. 2008. View Article : Google Scholar : PubMed/NCBI
|
3
|
Zhang DQ, Guo Q, Zhu JH and Chen WC:
Increase of cyclooxygenase-2 inhibition with celecoxib combined
with 5-FU enhances tumor cell apoptosis and antitumor efficacy in a
subcutaneous implantation tumor model of human colon cancer. World
J Surg Oncol. 11:162013. View Article : Google Scholar : PubMed/NCBI
|
4
|
Cheng L, Zhang M, Zhang P, Song Z, Ma Z
and Qu H: Silver complexation and tandem mass spectrometry for
differentiation of triterpenoid saponins from the roots of
Pulsatilla chinensis (Bunge) Regel. Rapid Commun Mass
Spectrom. 22:3783–3790. 2008. View
Article : Google Scholar : PubMed/NCBI
|
5
|
Xu QM, Shu Z, He WJ, Chen LY, Yang SL,
Yang G, Liu YL and Li XR: Antitumor activity of Pulsatilla
chinensis (Bunge) Regel saponins in human liver tumor 7402
cells in vitro and in vivo. Phytomedicine. 19:293–300. 2012.
View Article : Google Scholar : PubMed/NCBI
|
6
|
Liu Q, Chen W, Jiao Y, Hou J, Wu Q, Liu Y
and Qi X: Pulsatilla saponin A, an active molecule from
Pulsatilla chinensis, induces cancer cell death and inhibits
tumor growth in mouse xenograft models. J Surg Res. 188:387–395.
2014. View Article : Google Scholar : PubMed/NCBI
|
7
|
Qi X, Chen Z, Liu D, Cen J and Gu M:
Expression of Dlk1 gene in myelodysplastic syndrome determined by
microarray, and its effects on leukemia cells. Int J Mol Med.
22:61–68. 2008.PubMed/NCBI
|
8
|
Li Z, Qiu HY, Jiao Y, Cen JN, Fu CM, Hu
SY, Zhu MQ, Wu DP and Qi XF: Growth and differentiation effects of
Homer3 on a leukemia cell line. Asian Pac J Cancer Prev.
14:2525–2528. 2013. View Article : Google Scholar : PubMed/NCBI
|
9
|
Debatin K: Activation of apoptosis
pathways by anticancer treatment. Toxicol Lett. 112–113:41–48.
2000. View Article : Google Scholar
|
10
|
Iwaizumi M, Tseng-Rogenski S and Carethers
JM: DNA mismatch repair proficiency executing 5-fluorouracil
cytotoxicity in colorectal cancer cells. Cancer Biol Ther.
12:756–764. 2011. View Article : Google Scholar : PubMed/NCBI
|