MicroRNA-1258: An invasion and metastasis regulator that targets heparanase in gastric cancer Corrigendum in /10.3892/ol.2021.13103

Shi,Jinxin; Chen,Ping; Sun,Jingxu; Song,Yongxi; Ma,Bin; Gao,Peng; Chen,Xiaowan; Wang,Zhenning

doi:10.3892/ol.2017.5886

MicroRNA-1258: An invasion and metastasis regulator that targets heparanase in gastric cancer

Corrigendum in: /10.3892/ol.2021.13103

Authors:
- Jinxin Shi
- Ping Chen
- Jingxu Sun
- Yongxi Song
- Bin Ma
- Peng Gao
- Xiaowan Chen
- Zhenning Wang
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Affiliations: Department of Surgical Oncology and General Surgery, First Hospital of China Medical University, Shenyang, Liaoning 110001, P.R. China
Published online on: March 22, 2017 https://doi.org/10.3892/ol.2017.5886
Pages: 3739-3745

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Abstract

Numerous microRNAs (miRNAs/miRs) are involved in suppressing or promoting the formation of cancer. However, to the best of our knowledge, the role of miRNA‑1258 in gastric cancer (GC) has not previously been investigated. Our previous study demonstrated an increased expression of heparanase (HPSE) in GC tissues and HPSE‑facilitated invasion and metastasis of GC cells. Consequently, in the present study, the function of miR‑1258 in the invasion and metastasis of GC cells was investigated to determine whether miR‑1258 is associated with GC through HPSE. Reverse transcription-quantitative polymerase chain reaction (RT‑qPCR) was used to determine miR‑1258 expression in GC cell lines and tissues. A Transwell cell invasion assay and an MTT proliferation assay were used to investigate the effects of miR‑1258 on the invasion and proliferation of one of the cell lines, SGC‑7901 cells, in vitro. The effect of miR‑1258 on SGC‑7901 cell metastasis was investigated using an in vivo tumor metastasis formation assay. Western blot analysis and RT‑qPCR were used to investigate whether HPSE was a target of miR‑1258 in GC. The expression of miR‑1258 was significantly downregulated in 3 GC cell lines and 116 GC tissues compared with controls (all P<0.001). An association was identified between decreased miR‑1258 expression level and increased age (P=0.042), advanced pathological tumor stage (P=0.027) and positive lymphatic vessel invasion (P=0.044) in patients with GC. Furthermore, the upregulation of miR‑1258 expression suppressed SGC‑7901 cell invasion in vitro (P<0.001) and inhibited SGC‑7901 cell metastasis in vivo (P=0.016). The western blot analysis and RT‑qPCR results indicated that miR‑1258 downregulated the expression of HPSE at the translational level. The results of the present study indicate that miR‑1258 acts as a tumor suppressor to inhibit invasion and metastasis by targeting HPSE. Therefore, miR‑1258 may serve as a novel biomarker and therapeutic target in the treatment of GC.

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