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Article

MicroRNA-29a functions as a potential tumor suppressor through directly targeting CDC42 in non-small cell lung cancer

  • Authors:
    • Yongqiang Li
    • Zhi Wang
    • Yijiang Li
    • Ruijun Jing
  • View Affiliations / Copyright

    Affiliations: Department of Emergency, The Second Affiliated Hospital of Xi'an Medical University, Xi'an, Shaanxi 710038, P.R. China, Department of Thoracic Surgery, The Second Affiliated Hospital of Xi'an Medical University, Xi'an, Shaanxi 710038, P.R. China
  • Pages: 3896-3904
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    Published online on: March 22, 2017
       https://doi.org/10.3892/ol.2017.5888
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Abstract

The expression and function of microRNA-29a (miR-29a) have been investigated in various types of cancer. In the present study, the expression, function and underlying molecular mechanism of miR‑29a were investigated in non‑small cell lung cancer (NSCLC). The expression level of miR-29a in NSCLC was determined using reverse transcription‑quantitative polymerase chain reaction (RT-qPCR). Cell proliferation, migration and invasion ability were determined using Cell Counting Kit‑8, cell migration and invasion assays, respectively. Bioinformatics analysis and dual‑luciferase reporter assays were performed to determine whether cell division cycle 42 (CDC42) is a direct target gene of miR‑29a. To assess CDC42 mRNA and protein expression following transfection with miR‑29a, RT‑qPCR and western blotting were performed. Following knockdown of CDC42, functional assays were performed to investigate the roles of CDC42 in NSCLC. The results demonstrated that miR‑29a was downregulated in NSCLC and the decreased expression level of miR‑29a was significantly associated with advanced tumor‑node‑metastasis classification and metastasis. In addition, upregulation of miR‑29a inhibited cell proliferation, migration and invasion in NSCLC, whereas downregulation of miR‑29a had the opposite effects. Furthermore, CDC42 was identified as a direct target gene of miR‑29a in vitro. miR‑29a was demonstrated to function as a tumor suppressor in NSCLC by directly targeting CDC42 and may be investigated further as a target therapy for NSCLC.
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Copy and paste a formatted citation
Spandidos Publications style
Li Y, Wang Z, Li Y and Jing R: MicroRNA-29a functions as a potential tumor suppressor through directly targeting CDC42 in non-small cell lung cancer. Oncol Lett 13: 3896-3904, 2017.
APA
Li, Y., Wang, Z., Li, Y., & Jing, R. (2017). MicroRNA-29a functions as a potential tumor suppressor through directly targeting CDC42 in non-small cell lung cancer. Oncology Letters, 13, 3896-3904. https://doi.org/10.3892/ol.2017.5888
MLA
Li, Y., Wang, Z., Li, Y., Jing, R."MicroRNA-29a functions as a potential tumor suppressor through directly targeting CDC42 in non-small cell lung cancer". Oncology Letters 13.5 (2017): 3896-3904.
Chicago
Li, Y., Wang, Z., Li, Y., Jing, R."MicroRNA-29a functions as a potential tumor suppressor through directly targeting CDC42 in non-small cell lung cancer". Oncology Letters 13, no. 5 (2017): 3896-3904. https://doi.org/10.3892/ol.2017.5888
Copy and paste a formatted citation
x
Spandidos Publications style
Li Y, Wang Z, Li Y and Jing R: MicroRNA-29a functions as a potential tumor suppressor through directly targeting CDC42 in non-small cell lung cancer. Oncol Lett 13: 3896-3904, 2017.
APA
Li, Y., Wang, Z., Li, Y., & Jing, R. (2017). MicroRNA-29a functions as a potential tumor suppressor through directly targeting CDC42 in non-small cell lung cancer. Oncology Letters, 13, 3896-3904. https://doi.org/10.3892/ol.2017.5888
MLA
Li, Y., Wang, Z., Li, Y., Jing, R."MicroRNA-29a functions as a potential tumor suppressor through directly targeting CDC42 in non-small cell lung cancer". Oncology Letters 13.5 (2017): 3896-3904.
Chicago
Li, Y., Wang, Z., Li, Y., Jing, R."MicroRNA-29a functions as a potential tumor suppressor through directly targeting CDC42 in non-small cell lung cancer". Oncology Letters 13, no. 5 (2017): 3896-3904. https://doi.org/10.3892/ol.2017.5888
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