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Article

Increased methylation of human papillomavirus type 16 DNA is associated with the severity of cervical lesions in infected females from northeast China

  • Authors:
    • Wei Wang
    • Zhengrong Sun
    • Jianhua Liu
    • Guili Wang
    • Zhitao Lu
    • Weiqiang Zhou
    • Te Qi
    • Qiang Ruan
  • View Affiliations / Copyright

    Affiliations: Virus Laboratory, The Affiliated Shengjing Hospital, China Medical University, Shenyang, Liaoning 110004, P.R. China, BioBank, The Affiliated Shengjing Hospital, China Medical University, Shenyang, Liaoning 110004, P.R. China
  • Pages: 3809-3816
    |
    Published online on: March 24, 2017
       https://doi.org/10.3892/ol.2017.5903
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Abstract

Hypermethylation of the cytosine‑phosphate‑guanine (CpG) sites located at the 3'‑major capsid protein L1 (3'L1) and the long control region (LCR) of the human papillomavirus (HPV) genome may be associated with the progression of cervical cancer (CC). However, the methylation status of the LCR of HPV type 16 DNA remains to be elucidated in an infected Chinese population. The aim of the present study was to investigate the association between methylation of the HPV 16 L1 gene and LCR, and the severity of cervical lesions in infected female patients. Therefore, bisulfite modification, polymerase chain reaction amplification and sequencing were used to analyze 122 HPV 16‑positive clinical cervical swabs obtained from patients in northeastern China. The proportion of methylated samples at each of the 7 CpG sites within the 3'‑L1/5'‑LCR and 5 CpG sites within the promoter region was significantly increased in patients with CC, compared with that observed in high‑grade squamous intraepithelial lesions (HSIL) and normal tissue/low‑grade intraepithelial lesions (LSIL) (χ2 test, P<0.01). The mean methylation frequencies of the CpG sites 7,089 and 7,143 exhibited an area under the curve value of 0.822 [95% confidence interval (CI)=0.733‑0.911] for distinguishing CC from other lesions, 0.787 (95% CI=0.700‑0.874) for distinguishing normal/LSIL from HSIL and CC, and 0.763 (95% CI=0.652‑0.874) for distinguishing CC from HSIL. These results suggest that the methylation of CpG sites within the HPV 16 3'‑L1 and LCR region is correlated with the severity of cervical lesions. Quantification of HPV DNA methylation in the L1 gene and promoter region appears to provide a promising novel marker for distinguishing between normal tissue/LSIL, HSIL and CC in a Chinese population.
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1 

Murakami I, Fujii T, Dan K, Saito M, Ohno A, Iwata T and Aoki D: Methylation of human papillomavirus-52 and −58 is a candidate biomarker in cervical neoplasia. J Clin Virol. 58:149–154. 2013. View Article : Google Scholar : PubMed/NCBI

2 

Marongiu L, Godi A, Parry JV and Beddows S: Human Papillomavirus 16, 18, 31 and 45 viral load, integration and methylation status stratified by cervical disease stage. BMC Cancer. 14:3842014. View Article : Google Scholar : PubMed/NCBI

3 

Schiffman M, Castle PE, Jeronimo J, Rodriguez AC and Wacholder S: Human papillomavirus and cervical cancer. Lancet. 370:890–907. 2007. View Article : Google Scholar : PubMed/NCBI

4 

Clarke MA, Wentzensen N, Mirabello L, Ghosh A, Wacholder S, Harari A, Lorincz A, Schiffman M and Burk RD: Human papillomavirus DNA methylation as a potential biomarker for cervical cancer. Cancer Epidemiol Biomarkers Prev. 21:2125–2137. 2012. View Article : Google Scholar : PubMed/NCBI

5 

Kalantari M, Osann K, Calleja-Macias IE, Kim S, Yan B, Jordan S, Chase DM, Tewari KS and Bernard HU: Methylation of human papillomavirus 16, 18, 31, and 45 L2 and L1 genes and the cellular DAPK gene: Considerations for use as biomarkers of the progression of cervical neoplasia. Virology. 448:314–321. 2014. View Article : Google Scholar : PubMed/NCBI

6 

Whiteside MA, Siegel EM and Unger ER: Human papillomavirus and molecular considerations for cancer risk. Cancer. 113:(10 Suppl). S2981–S2994. 2008. View Article : Google Scholar

7 

Kim SS, Suh DS, Kim KH, Yoon MS and Choi KU: Clinicopathological significance of atypical glandular cells on Pap smear. Obstet Gynecol Sci. 56:76–83. 2013. View Article : Google Scholar : PubMed/NCBI

8 

Zhou J, Tomashefski JF Jr, Sawady J, Ferrer H and Khiyami A: The diagnostic value of the ThinPrep pap test in endometrial carcinoma: A prospective study with histological follow-up. Diagn Cytopathol. 41:408–412. 2013. View Article : Google Scholar : PubMed/NCBI

9 

De Strooper LM, van Zummeren M, Steenbergen RD, Bleeker MC, Hesselink AT, Wisman GB, Snijders PJ, Heideman DA and Meijer CJ: CADM1, MAL and miR124-2 methylation analysis in cervical scrapes to detect cervical and endometrial cancer. J Clin Pathol. 67:1067–1071. 2014. View Article : Google Scholar : PubMed/NCBI

10 

Arbyn M, Ronco G, Anttila A, Meijer CJ, Poljak M, Ogilvie G, Koliopoulos G, Naucler P, Sankaranarayanan R and Peto J: Evidence regarding human papillomavirus testing in secondary prevention of cervical cancer. Vaccine. 30:(Suppl 5). F88–F99. 2012. View Article : Google Scholar : PubMed/NCBI

11 

Bryant D, Tristram A, Liloglou T, Hibbitts S, Fiander A and Powell N: Quantitative measurement of human papillomavirus type 16 L1/L2 DNA methylation correlates with cervical disease grade. J Clin Virol. 59:24–29. 2014. View Article : Google Scholar : PubMed/NCBI

12 

Baylin SB and Herman JG: DNA hypermethylation in tumorigenesis: Epigenetics joins genetics. Trends Genet. 16:168–174. 2000. View Article : Google Scholar : PubMed/NCBI

13 

Shivapurkar N, Sherman ME, Stastny V, Echebiri C, Rader JS, Nayar R, Bonfiglio TA, Gazdar AF and Wang SS: Evaluation of candidate methylation markers to detect cervical neoplasia. Gynecol Oncol. 107:549–553. 2007. View Article : Google Scholar : PubMed/NCBI

14 

Kalantari M, Villa LL, Calleja-Macias IE and Bernard HU: Human papillomavirus-16 and −18 in penile carcinomas: DNA methylation, chromosomal recombination and genomic variation. Int J Cancer. 123:1832–1840. 2008. View Article : Google Scholar : PubMed/NCBI

15 

Wentzensen N, Sun C, Ghosh A, Kinney W, Mirabello L, Wacholder S, Shaber R, LaMere B, Clarke M, Lorincz AT, et al: Methylation of HPV18, HPV31, and HPV45 genomes and cervical intraepithelial neoplasia grade III. J Natl Cancer Inst. 104:1738–1749. 2012. View Article : Google Scholar : PubMed/NCBI

16 

Sun Z, Lu Z, Liu J, Wang G, Zhou W, Yang L, Liu C, Wang B and Ruan Q: Genetic variations of E6 and long control region of human papillomavirus type 16 from patients with cervical lesion in Liaoning, China. BMC Cancer. 13:4592013. View Article : Google Scholar : PubMed/NCBI

17 

Verma I, Jain V and Kaur T: Application of bethesda system for cervical cytology in unhealthy cervix. J Clin Diagn Res. 8:OC26–OC30. 2014.PubMed/NCBI

18 

Li LC and Dahiya R: MethPrimer: Designing primers for methylation PCRs. Bioinformatics. 18:1427–1431. 2002. View Article : Google Scholar : PubMed/NCBI

19 

Xi LF, Jiang M, Shen Z, Hulbert A, Zhou XH, Lin YY, Kiviat NB and Koutsky LA: Inverse association between methylation of human papillomavirus type 16 DNA and risk of cervical intraepithelial neoplasia grades II or III. PLoS One. 6:e238972011. View Article : Google Scholar : PubMed/NCBI

20 

Zhu WG, Srinivasan K, Dai Z, Duan W, Druhan LJ, Ding H, Yee L, Villalona-Calero MA, Plass C and Otterson GA: Methylation of adjacent CpG sites affects Sp1/Sp3 binding and activity in the p21 (Cip1) promoter. Mol Cell Biol. 23:4056–4065. 2003. View Article : Google Scholar : PubMed/NCBI

21 

Hong D, Ye F, Lu W, Hu Y, Wan X, Chen Y and Xie X: Methylation status of the long control region of HPV 16 in clinical cervical specimens. Mol Med Rep. 1:555–560. 2008.PubMed/NCBI

22 

Bryant D, Onions T, Raybould R, Jones S, Tristram A, Hibbitts S, Fiander A and Powell N: Increased methylation of human papillomavirus type 16 DNA correlates with viral integration in Vulval Intraepithelial Neoplasia. J Clin Virol. 61:393–399. 2014. View Article : Google Scholar : PubMed/NCBI

23 

Ding DC, Chiang MH, Lai HC, Hsiung CA, Hsieh CY and Chu TY: Methylation of the long control region of HPV16 is related to the severity of cervical neoplasia. Eur J Obstet Gynecol Reprod Biol. 147:215–220. 2009. View Article : Google Scholar : PubMed/NCBI

24 

Doerfler W, Remus R, Müller K, Heller H, Hohlweg U and Schubbert R: The fate of foreign DNA in mammalian cells and organisms. Dev Biol (Basel). 106:89–97; discussion 143–160. 2001.PubMed/NCBI

25 

Daniel B, Mukherjee G, Seshadri L, Vallikad E and Krishna S: Changes in the physical state and expression of human papillomavirus type 16 in the progression of cervical intraepithelial neoplasia lesions analysed by PCR. J Gen Virol. 76:2589–2593. 1995. View Article : Google Scholar : PubMed/NCBI

26 

Daniel B, Rangarajan A, Mukherjee G, Vallikad E and Krishna S: The link between integration and expression of human papillomavirus type 16 genomes and cellular changes in the evolution of cervical intraepithelial neoplastic lesions. J Gen Virol. 78:1095–1101. 1997. View Article : Google Scholar : PubMed/NCBI

27 

Schwarz E, Freese UK, Gissmann L, Mayer W, Roggenbuck B, Stremlau A and zur Hausen H: Structure and transcription of human papillomavirus sequences in cervical carcinoma cells. Nature. 314:111–114. 1985. View Article : Google Scholar : PubMed/NCBI

28 

Vinokurova S, Wentzensen N, Kraus I, Klaes R, Driesch C, Melsheimer P, Kisseljov F, Dürst M, Schneider A and von Knebel Doeberitz M: Type-dependent integration frequency of human papillomavirus genomes in cervical lesions. Cancer Res. 68:307–313. 2008. View Article : Google Scholar : PubMed/NCBI

29 

Dutta S, Chakraborty C, Dutta AK, Mandal RK, Roychoudhury S, Basu P and Panda C: Physical and methylation status of human papillomavirus 16 in asymptomatic cervical infections changes with malignant transformation. J Clin Pathol. 68:206–211. 2015. View Article : Google Scholar : PubMed/NCBI

30 

Qiu C, Zhi Y, Shen Y, Gong J, Li Y and Li X: High-resolution melting analysis of HPV-16L1 gene methylation: A promising method for prognosing cervical cancer. Clin Biochem. 48:855–859. 2015. View Article : Google Scholar : PubMed/NCBI

31 

Bird A: DNA methylation patterns and epigenetic memory. Genes Dev. 16:6–21. 2002. View Article : Google Scholar : PubMed/NCBI

32 

Fuks F: DNA methylation and histone modifications: Teaming up to silence genes. Curr Opin Genet Dev. 15:490–495. 2005. View Article : Google Scholar : PubMed/NCBI

33 

Mino A, Onoda N, Yashiro M, Aya M, Fujiwara I, Kubo N, Sawada T, Ohira M, Kato Y and Hirakawa K: Frequent p16 CpG island hypermethylation in primary remnant gastric cancer suggesting an independent carcinogenic pathway. Oncol Rep. 15:615–620. 2006.PubMed/NCBI

34 

Münger K, Baldwin A, Edwards KM, Hayakawa H, Nguyen CL, Owens M, Grace M and Huh K: Mechanisms of human papillomavirus-induced oncogenesis. J Virol. 78:11451–11460. 2004. View Article : Google Scholar : PubMed/NCBI

35 

Yang HJ: Aberrant DNA methylation in cervical carcinogenesis. Chin J Cancer. 32:42–48. 2013. View Article : Google Scholar : PubMed/NCBI

36 

Tan SH, Leong LE, Walker PA and Bernard HU: The human papillomavirus type 16 E2 transcription factor binds with low cooperativity to two flanking sites and represses the E6 promoter through displacement of Sp1 and TFIID. J Virol. 68:6411–6420. 1994.PubMed/NCBI

37 

Kalantari M, Calleja-Macias IE, Tewari D, Hagmar B, Lie K, Barrera-Saldana HA, Wiley DJ and Bernard HU: Conserved methylation patterns of human papillomavirus type 16 DNA in asymptomatic infection and cervical neoplasia. J Virol. 78:12762–12772. 2004. View Article : Google Scholar : PubMed/NCBI

38 

Rajeevan MS, Swan DC, Nisenbaum R, Lee DR, Vernon SD, Ruffin MT, Horowitz IR, Flowers LC, Kmak D, Tadros T, et al: Epidemiologic and viral factors associated with cervical neoplasia in HPV-16-positive women. Int J Cancer. 115:114–120. 2005. View Article : Google Scholar : PubMed/NCBI

39 

Mirabello L, Schiffman M, Ghosh A, Rodriguez AC, Vasiljevic N, Wentzensen N, Herrero R, Hildesheim A, Wacholder S, Scibior-Bentkowska D, et al: Elevated methylation of HPV16 DNA is associated with the development of high grade cervical intraepithelial neoplasia. Int J Cancer. 132:1412–1422. 2013. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Wang W, Sun Z, Liu J, Wang G, Lu Z, Zhou W, Qi T and Ruan Q: Increased methylation of human papillomavirus type 16 DNA is associated with the severity of cervical lesions in infected females from northeast China. Oncol Lett 13: 3809-3816, 2017.
APA
Wang, W., Sun, Z., Liu, J., Wang, G., Lu, Z., Zhou, W. ... Ruan, Q. (2017). Increased methylation of human papillomavirus type 16 DNA is associated with the severity of cervical lesions in infected females from northeast China. Oncology Letters, 13, 3809-3816. https://doi.org/10.3892/ol.2017.5903
MLA
Wang, W., Sun, Z., Liu, J., Wang, G., Lu, Z., Zhou, W., Qi, T., Ruan, Q."Increased methylation of human papillomavirus type 16 DNA is associated with the severity of cervical lesions in infected females from northeast China". Oncology Letters 13.5 (2017): 3809-3816.
Chicago
Wang, W., Sun, Z., Liu, J., Wang, G., Lu, Z., Zhou, W., Qi, T., Ruan, Q."Increased methylation of human papillomavirus type 16 DNA is associated with the severity of cervical lesions in infected females from northeast China". Oncology Letters 13, no. 5 (2017): 3809-3816. https://doi.org/10.3892/ol.2017.5903
Copy and paste a formatted citation
x
Spandidos Publications style
Wang W, Sun Z, Liu J, Wang G, Lu Z, Zhou W, Qi T and Ruan Q: Increased methylation of human papillomavirus type 16 DNA is associated with the severity of cervical lesions in infected females from northeast China. Oncol Lett 13: 3809-3816, 2017.
APA
Wang, W., Sun, Z., Liu, J., Wang, G., Lu, Z., Zhou, W. ... Ruan, Q. (2017). Increased methylation of human papillomavirus type 16 DNA is associated with the severity of cervical lesions in infected females from northeast China. Oncology Letters, 13, 3809-3816. https://doi.org/10.3892/ol.2017.5903
MLA
Wang, W., Sun, Z., Liu, J., Wang, G., Lu, Z., Zhou, W., Qi, T., Ruan, Q."Increased methylation of human papillomavirus type 16 DNA is associated with the severity of cervical lesions in infected females from northeast China". Oncology Letters 13.5 (2017): 3809-3816.
Chicago
Wang, W., Sun, Z., Liu, J., Wang, G., Lu, Z., Zhou, W., Qi, T., Ruan, Q."Increased methylation of human papillomavirus type 16 DNA is associated with the severity of cervical lesions in infected females from northeast China". Oncology Letters 13, no. 5 (2017): 3809-3816. https://doi.org/10.3892/ol.2017.5903
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