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Article Open Access

The effect of aloe‑emodin‑induced photodynamic activity on the apoptosis of human gastric cancer cells: A pilot study

  • Authors:
    • Hai‑Dan Lin
    • Kai‑Ting Li
    • Qin‑Qin Duan
    • Qing Chen
    • Shi Tian
    • Ellie Shihng Meir Chu
    • Ding‑Qun Bai
  • View Affiliations / Copyright

    Affiliations: Department of Rehabilitation, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, P.R. China, Department of Gastroenterology, Chinese Medicine Hospital of Longquan, Chengdu, Sichuan 610100, P.R. China, Department of Medical Science, Tung Wah College, Hong Kong 999077, SAR, P.R. China
    Copyright: © Lin et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 3431-3436
    |
    Published online on: March 27, 2017
       https://doi.org/10.3892/ol.2017.5915
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Abstract

The aim of the present study was to explore the effect of aloe-emodin (AE)-induced photodynamic activity in human gastric cancer cells. AE was used as a photosensitizer to explore the effect of photodynamic therapy (PDT) in human gastric cancer cells (SGC‑7901). An MTT assay was used to detect the effect of AE‑induced PDT in optimal concentrations and illumination energy densities in human gastric cancer cells. Following AE‑induced PDT, morphological changes of the cells and the rate of cell death were evaluated by TUNEL assay and flow cytometry, respectively. The expression levels of caspase‑9 and caspase‑3 were determined by western blot analysis. The AE and AE‑induced PDT demonstrated a significant inhibitive effect on the proliferation of human gastric cancer cells in dose‑dependent and energy‑dependent manners. For subsequent experiments, 10 µM AE and 12.8 J/cm2 illumination energy density were used. Typical morphological changes of apoptosis were observed in the cells using a TUNEL assay 12 h subsequent to AE‑induced PDT. The percentage of apoptotic cells treated with AE‑induced PDT significantly increased when compared with the control group, the 10 µM AE group and the illumination group (P<0.05). Upregulation of caspase‑9 and caspase‑3 protein levels was also observed following AE‑induced PDT. The present study revealed that 10 µM AE‑induced PDT had an inhibitory effect on human gastric cancer cells, and it may induce cell apoptosis by upregulating caspase‑9 and caspase‑3, which indicated that the mitochondrial pathway may be involved. AE‑induced PDT has the potential to be a novel therapy for the treatment of human gastric cancer. However, further investigations are required.
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1 

Nowak-Stępniowska A, Wiktorska K, Małecki M, Milczarek M, Lubelska K and Padzik-Graczyk A: Cytotoxicity of PP(Arg)2- and PP(Ala)2(Arg)2-based photodynamic therapy and early stage of apoptosis induction in human breast cancers in vitro. Acta Biochim Pol. 59:603–611. 2012.PubMed/NCBI

2 

Li JH, Chen ZQ, Huang Z, Zhan Q, Ren FB, Liu JY, Yue W and Wang Z: In vitro study of low intensity ultrasound combined with different doses of PDT: Effects on C6 glioma cells. Oncol Lett. 5:702–706. 2013.PubMed/NCBI

3 

Qumseya BJ, David W and Wolfsen HC: Photodynamic therapy for barrett's esophagus and esophageal carcinoma. Clin Endosc. 46:30–37. 2013. View Article : Google Scholar : PubMed/NCBI

4 

Buytaert E, Dewaele M and Agostinis P: Molecular effectors of multiple cell death pathways initiated by photodynamic therapy. Biochim Biophys Acta. 1776:86–107. 2007.PubMed/NCBI

5 

Verma S, Watt GM, Mai Z and Hasan T: Strategies for enhanced photodynamic therapy effects. Photochem Photobiol. 83:996–1005. 2007. View Article : Google Scholar : PubMed/NCBI

6 

Robertson CA, Evans DH and Abrahamse H: Photodynamic therapy (PDT): A short review on cellular mechanisms and cancer research applications for PDT. J Photochem Photobiol B. 96:1–8. 2009. View Article : Google Scholar : PubMed/NCBI

7 

Kushibiki T, Hirasawa T, Okawa S and Ishihara M: Responses of cancer cells induced by photodynamic therapy. J Healthc Eng. 4:87–108. 2013. View Article : Google Scholar : PubMed/NCBI

8 

Pecere T, Gazzola MV, Mucignat C, Parolin C, Vecchia FD, Cavaggioni A, Basso G, Diaspro A, Salvato B, Carli M and Palù G: Aloe-emodin is a new type of anticancer agent with selective activity against neuroectodermal tumors. Cancer Res. 60:2800–2804. 2000.PubMed/NCBI

9 

Chiu TH, Lai WW, Hsia TC, Yang JS, Lai TY, Wu PP, Ma CY, Yeh CC, Ho CC, Lu HF, et al: Aloe-emodin induces cell death through S-phase arrest and caspase-dependent pathways in human tongue squamous cancer SCC-4 cells. Anticancer Res. 29:4503–4511. 2009.PubMed/NCBI

10 

Lin KY and Uen YH: Aloe-emodin, an anthraquinone, in vitro inhibits proliferation and induces apoptosis in human colon carcinoma cells. Oncol Lett. 1:541–547. 2010.PubMed/NCBI

11 

Zhu S, Jin J, Wang Y, Ouyang Z, Xi C, Li J, Qiu Y, Wan J, Huang M and Huang Z: The endoplasmic reticulum stress response is involved in apoptosis induced by aloe-emodin in HK-2 cells. Food Chem Toxicol. 50:1149–1158. 2012. View Article : Google Scholar : PubMed/NCBI

12 

Vath P, Wamer WG and Falvey DE: Photochemistry and phototoxicity of aloe emodin. Photochem Photobiol. 75:346–352. 2002. View Article : Google Scholar : PubMed/NCBI

13 

Bai DQ, Yu LH, Xia XS, Yu HP, Jiang Q, Tan Y, Zeng XB and Xu CS: Study on spectral properties of aloe-emodin in different solvents. Laser J. 28:83–85. 2007.

14 

Lee HZ, Yang WH, Hour MJ, Wu CY, Peng WH, Bao BY, Han PH and Bau DT: Photodynamic activity of aloe-emodin induces resensitization of lung cancer cells to anoikis. Eur J Pharmacol. 648:50–58. 2010. View Article : Google Scholar : PubMed/NCBI

15 

Cardenas C, Quesada AR and Medina MA: Evaluation of the anti-angiogenic effect of aloe-emodin. Cell Mol Life Sci. 63:3083–3089. 2006. View Article : Google Scholar : PubMed/NCBI

16 

Juzeniene A and Moan J: The history of PDT in Norway Part II. Recent advances in general PDT and ALA-PDT. Photodiagnosis Photodyn Ther. 4:80–87. 2007. View Article : Google Scholar : PubMed/NCBI

17 

MacCormack MA: Photodynamic therapy in dermatology: An update on applications and outcomes. Semin Cutan Med Surg. 27:52–62. 2008. View Article : Google Scholar : PubMed/NCBI

18 

Nicholson DW: Caspase structure, proteolytic substrates, and function during apoptotic cell death. Cell Death Differ. 6:1028–1042. 1999. View Article : Google Scholar : PubMed/NCBI

19 

Lam M, Oleinick NL and Nieminen AL: Photodynamic therapy-induced apoptosis in epidermoid carcinoma cells. Reactive oxygen species and mitochondrial inner membrane permeabilization. J Biol Chem. 276:47379–47386. 2001. View Article : Google Scholar : PubMed/NCBI

20 

Hilf R: Mitochondria are targets of photodynamic therapy. J Bioenerg Biomembr. 39:85–89. 2007. View Article : Google Scholar : PubMed/NCBI

21 

Buggiani G, Troiano M, Rossi R and Lotti T: Photodynamic therapy: Off-label and alternative use in dermatological practice. Photodiagnosis Photodyn Ther. 5:134–138. 2008. View Article : Google Scholar : PubMed/NCBI

22 

Li KT, Duan QQ, Chen Q, He JW, Tian S, Lin HD, Gao Q and Bai DQ: The effect of aloe emodin-encapsulated nanoliposome-mediated r-caspase-3 gene transfection and photodynamic therapy on human gastric cancer cells. Cancer Med. 5:361–369. 2015. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Lin HD, Li KT, Duan QQ, Chen Q, Tian S, Chu ES and Bai DQ: The effect of aloe‑emodin‑induced photodynamic activity on the apoptosis of human gastric cancer cells: A pilot study. Oncol Lett 13: 3431-3436, 2017.
APA
Lin, H., Li, K., Duan, Q., Chen, Q., Tian, S., Chu, E.S., & Bai, D. (2017). The effect of aloe‑emodin‑induced photodynamic activity on the apoptosis of human gastric cancer cells: A pilot study. Oncology Letters, 13, 3431-3436. https://doi.org/10.3892/ol.2017.5915
MLA
Lin, H., Li, K., Duan, Q., Chen, Q., Tian, S., Chu, E. S., Bai, D."The effect of aloe‑emodin‑induced photodynamic activity on the apoptosis of human gastric cancer cells: A pilot study". Oncology Letters 13.5 (2017): 3431-3436.
Chicago
Lin, H., Li, K., Duan, Q., Chen, Q., Tian, S., Chu, E. S., Bai, D."The effect of aloe‑emodin‑induced photodynamic activity on the apoptosis of human gastric cancer cells: A pilot study". Oncology Letters 13, no. 5 (2017): 3431-3436. https://doi.org/10.3892/ol.2017.5915
Copy and paste a formatted citation
x
Spandidos Publications style
Lin HD, Li KT, Duan QQ, Chen Q, Tian S, Chu ES and Bai DQ: The effect of aloe‑emodin‑induced photodynamic activity on the apoptosis of human gastric cancer cells: A pilot study. Oncol Lett 13: 3431-3436, 2017.
APA
Lin, H., Li, K., Duan, Q., Chen, Q., Tian, S., Chu, E.S., & Bai, D. (2017). The effect of aloe‑emodin‑induced photodynamic activity on the apoptosis of human gastric cancer cells: A pilot study. Oncology Letters, 13, 3431-3436. https://doi.org/10.3892/ol.2017.5915
MLA
Lin, H., Li, K., Duan, Q., Chen, Q., Tian, S., Chu, E. S., Bai, D."The effect of aloe‑emodin‑induced photodynamic activity on the apoptosis of human gastric cancer cells: A pilot study". Oncology Letters 13.5 (2017): 3431-3436.
Chicago
Lin, H., Li, K., Duan, Q., Chen, Q., Tian, S., Chu, E. S., Bai, D."The effect of aloe‑emodin‑induced photodynamic activity on the apoptosis of human gastric cancer cells: A pilot study". Oncology Letters 13, no. 5 (2017): 3431-3436. https://doi.org/10.3892/ol.2017.5915
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