Enhancement of antitumor immunity by combination of anti-CTLA-4 antibody and radioimmunotherapy through the suppression of Tregs

  • Authors:
    • Cheol‑Hun Son
    • Jaeho Bae
    • Hong‑Rae Lee
    • Kwangmo Yang
    • You‑Soo Park
  • View Affiliations

  • Published online on: March 28, 2017     https://doi.org/10.3892/ol.2017.5933
  • Pages: 3781-3786
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Abstract

Cytotoxic T lymphocyte antigen-4 (CTLA-4) is expressed during cluster of differentiation (CD)4+ T-cell activation and terminates immune responses by interrupting CD28‑enhanced activation. In addition, CTLA‑4 is known to be constitutively expressed in regulatory T-cells (Tregs) and to contribute to immune suppression by enhancing the suppressive function of Tregs. However, the molecular mechanisms underlying CTLA‑4‑mediated Treg suppression remains incompletely understood. Furthermore, it is uncertain whether the in vivo immune suppressive functions of CTLA‑4 are mediated only by a reduction in the level of conventional T‑cell activity, or enhancement of Treg function. The present study demonstrated that combination therapy with an anti‑CTLA‑4 monoclonal antibody and dendritic cell‑mediated radioimmunotherapy (IR/DC) was able to promote an antitumor response and influence Treg function in a mouse model of lung cancer. Cell surface markers, including CTLA‑4, CD25 and CD4, were analyzed using flow cytometry, and T‑cell activities were measured using ELISPOT and cytotoxicity assays. It was revealed that anti‑CTLA‑4 combined treatment with IR/DC immunotherapy may execute a more powerful and effective anti‑tumor immunity through the inhibition of Treg function.
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May-2017
Volume 13 Issue 5

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Son CH, Bae J, Lee HR, Yang K and Park YS: Enhancement of antitumor immunity by combination of anti-CTLA-4 antibody and radioimmunotherapy through the suppression of Tregs. Oncol Lett 13: 3781-3786, 2017
APA
Son, C., Bae, J., Lee, H., Yang, K., & Park, Y. (2017). Enhancement of antitumor immunity by combination of anti-CTLA-4 antibody and radioimmunotherapy through the suppression of Tregs. Oncology Letters, 13, 3781-3786. https://doi.org/10.3892/ol.2017.5933
MLA
Son, C., Bae, J., Lee, H., Yang, K., Park, Y."Enhancement of antitumor immunity by combination of anti-CTLA-4 antibody and radioimmunotherapy through the suppression of Tregs". Oncology Letters 13.5 (2017): 3781-3786.
Chicago
Son, C., Bae, J., Lee, H., Yang, K., Park, Y."Enhancement of antitumor immunity by combination of anti-CTLA-4 antibody and radioimmunotherapy through the suppression of Tregs". Oncology Letters 13, no. 5 (2017): 3781-3786. https://doi.org/10.3892/ol.2017.5933