Identification of proteins with different abundance associated with cell migration and proliferation in leiomyoma interstitial fluid by proteomics

  • Authors:
    • Blendi Ura
    • Federica Scrimin
    • Cinzia Franchin
    • Giorgio Arrigoni
    • Danilo Licastro
    • Lorenzo Monasta
    • Giuseppe Ricci
  • View Affiliations

  • Published online on: March 29, 2017     https://doi.org/10.3892/ol.2017.5943
  • Pages: 3912-3920
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Abstract

Uterine leiomyoma is the most common female reproductive tract benign tumor. Little is known about protein composition and changes in the leiomyoma interstitial fluid (IF). The present study focused on changes in protein abundance in the IF of leiomyoma. Leiomyoma IFs and adjacent myometrial IFs were obtained and analyzed by two‑dimensional electrophoresis (2-DE) coupled with mass spectrometry and western blotting for 2‑DE data validation. A total of 25 unique proteins were observed to change significantly (P<0.05). Of these proteins with different abundance, 22 had not been previously identified in leiomyoma IF. In silico analysis predicted that three of these proteins were secreted via classical mechanisms, while 22 were secreted via non‑classical mechanisms. Ingenuity Pathway Analysis identified 17 proteins associated with cellular migration and proliferation. Among these, phosphoglycerate mutase 1 had not been previously associated with leiomyoma. The abundance of seven proteins was further validated by western blotting. A comparative proteomic approach identified a number of proteins associated with cellular migration and proliferation, with changes in abundance in IF likely to be involved in tumor development. Further studies will be required to investigate the role of these proteins in leiomyoma IF and their possible association with tumor development and growth.
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May-2017
Volume 13 Issue 5

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Ura B, Scrimin F, Franchin C, Arrigoni G, Licastro D, Monasta L and Ricci G: Identification of proteins with different abundance associated with cell migration and proliferation in leiomyoma interstitial fluid by proteomics. Oncol Lett 13: 3912-3920, 2017
APA
Ura, B., Scrimin, F., Franchin, C., Arrigoni, G., Licastro, D., Monasta, L., & Ricci, G. (2017). Identification of proteins with different abundance associated with cell migration and proliferation in leiomyoma interstitial fluid by proteomics. Oncology Letters, 13, 3912-3920. https://doi.org/10.3892/ol.2017.5943
MLA
Ura, B., Scrimin, F., Franchin, C., Arrigoni, G., Licastro, D., Monasta, L., Ricci, G."Identification of proteins with different abundance associated with cell migration and proliferation in leiomyoma interstitial fluid by proteomics". Oncology Letters 13.5 (2017): 3912-3920.
Chicago
Ura, B., Scrimin, F., Franchin, C., Arrigoni, G., Licastro, D., Monasta, L., Ricci, G."Identification of proteins with different abundance associated with cell migration and proliferation in leiomyoma interstitial fluid by proteomics". Oncology Letters 13, no. 5 (2017): 3912-3920. https://doi.org/10.3892/ol.2017.5943