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Article

CCAAT/enhancer-binding protein α decreases the viability of gastric cancer cells

  • Authors:
    • Minoru Tomizawa
    • Fuminobu Shinozaki
    • Yasufumi Motoyoshi
    • Takao Sugiyama
    • Shigenori Yamamoto
    • Naoki Ishige
  • View Affiliations / Copyright

    Affiliations: Department of Gastroenterology, National Hospital Organization, Shimoshizu Hospital, Yotsukaido, Chiba 284‑0003, Japan, Department of Radiology, National Hospital Organization, Shimoshizu Hospital, Yotsukaido, Chiba 284‑0003, Japan, Department of Neurology, National Hospital Organization, Shimoshizu Hospital, Yotsukaido, Chiba 284‑0003, Japan, Department of Rheumatology, National Hospital Organization, Shimoshizu Hospital, Yotsukaido, Chiba 284‑0003, Japan, Department of Pediatrics, National Hospital Organization, Shimoshizu Hospital, Yotsukaido, Chiba 284‑0003, Japan, Department of Neurosurgery, National Hospital Organization, Shimoshizu Hospital, Yotsukaido, Chiba 284‑0003, Japan
  • Pages: 4322-4326
    |
    Published online on: April 5, 2017
       https://doi.org/10.3892/ol.2017.5987
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Abstract

CCAAT/enhancer-binding protein (C/EBP) α, C/EBPβ and C/EBPδ are involved in inflammation and cell differentiation. In the present study, their roles in human gastric cancer cells were investigated. The human gastric cancer cell lines MKN45 and MKN74 were subjected to the reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR) to analyze the expression levels of C/EBPα,C/EBPβ and C/EBPδ. The cells were transfected with expression plasmids for either C/EBPα or C/EBPδ, and subjected to a 3-(4,5-dimethylthiazol-2-yl)‑5‑(3‑carboxymethoxyphenyl)‑2‑(4‑sulfophenyl)‑2H‑tetrazolium inner salt (MTS) assay and RT‑qPCR for analysis of cyclin D1 expression. Expression levels of C/EBPα and C/EBPδ were decreased in MKN45 and MKN74 cells compared with in normal gastric tissue. Expression levels of C/EBPβ were decreased in MKN45 cells and increased in MKN74 cells. Viability of MKN45 cells was decreased by C/EBPα and C/EBPδ. Viability of MKN74 cells was decreased by C/EBPα, but increased by C/EBPδ. Expression levels of cyclin D1 were decreased in association with C/EBPα and C/EBPδ overexpression in MKN45 cells. Expression levels of cyclin D1 were decreased in association with C/EBPα overexpression, but increased in association with C/EBPδ overexpression, in MKN74 cells. The results of the present study indicate that C/EBPα is potentially useful for the treatment of gastric cancer.
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Copy and paste a formatted citation
Spandidos Publications style
Tomizawa M, Shinozaki F, Motoyoshi Y, Sugiyama T, Yamamoto S and Ishige N: CCAAT/enhancer-binding protein α decreases the viability of gastric cancer cells. Oncol Lett 13: 4322-4326, 2017.
APA
Tomizawa, M., Shinozaki, F., Motoyoshi, Y., Sugiyama, T., Yamamoto, S., & Ishige, N. (2017). CCAAT/enhancer-binding protein α decreases the viability of gastric cancer cells. Oncology Letters, 13, 4322-4326. https://doi.org/10.3892/ol.2017.5987
MLA
Tomizawa, M., Shinozaki, F., Motoyoshi, Y., Sugiyama, T., Yamamoto, S., Ishige, N."CCAAT/enhancer-binding protein α decreases the viability of gastric cancer cells". Oncology Letters 13.6 (2017): 4322-4326.
Chicago
Tomizawa, M., Shinozaki, F., Motoyoshi, Y., Sugiyama, T., Yamamoto, S., Ishige, N."CCAAT/enhancer-binding protein α decreases the viability of gastric cancer cells". Oncology Letters 13, no. 6 (2017): 4322-4326. https://doi.org/10.3892/ol.2017.5987
Copy and paste a formatted citation
x
Spandidos Publications style
Tomizawa M, Shinozaki F, Motoyoshi Y, Sugiyama T, Yamamoto S and Ishige N: CCAAT/enhancer-binding protein α decreases the viability of gastric cancer cells. Oncol Lett 13: 4322-4326, 2017.
APA
Tomizawa, M., Shinozaki, F., Motoyoshi, Y., Sugiyama, T., Yamamoto, S., & Ishige, N. (2017). CCAAT/enhancer-binding protein α decreases the viability of gastric cancer cells. Oncology Letters, 13, 4322-4326. https://doi.org/10.3892/ol.2017.5987
MLA
Tomizawa, M., Shinozaki, F., Motoyoshi, Y., Sugiyama, T., Yamamoto, S., Ishige, N."CCAAT/enhancer-binding protein α decreases the viability of gastric cancer cells". Oncology Letters 13.6 (2017): 4322-4326.
Chicago
Tomizawa, M., Shinozaki, F., Motoyoshi, Y., Sugiyama, T., Yamamoto, S., Ishige, N."CCAAT/enhancer-binding protein α decreases the viability of gastric cancer cells". Oncology Letters 13, no. 6 (2017): 4322-4326. https://doi.org/10.3892/ol.2017.5987
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