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Article

Expression of regulatory factor X1 can predict the prognosis of breast cancer

  • Authors:
    • Masahiro Shibata
    • Mitsuro Kanda
    • Dai Shimizu
    • Haruyoshi Tanaka
    • Shinichi Umeda
    • Masamichi Hayashi
    • Takahiro Inaishi
    • Noriyuki Miyajima
    • Yayoi Adachi
    • Yuko Takano
    • Kenichi Nakanishi
    • Dai Takeuchi
    • Sumiyo Noda
    • Yasuhiro Kodera
    • Toyone Kikumori
  • View Affiliations / Copyright

    Affiliations: Department of Breast and Endocrine Surgery (Surgery II), Nagoya University Graduate School of Medicine, Showa‑ku, Nagoya 466‑8550, Japan , Department of Gastroenterological Surgery (Surgery II), Nagoya University Graduate School of Medicine, Showa‑ku, Nagoya 466‑8550, Japan
  • Pages: 4334-4340
    |
    Published online on: April 7, 2017
       https://doi.org/10.3892/ol.2017.6005
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Abstract

Breast cancer (BC) is the most common malignancy among women. Identifying novel biomarkers to predict prognosis accurately is important in managing this disease. The regulatory factor X1 (RFX1) gene is a member of the regulatory factor X gene family. Its protein reportedly downregulates the proto‑oncogene c‑myc, but its role in BC has been unclear. In this study, expression and methylation status of RFX1 were determined in BC cell lines. We then evaluated RFX1 mRNA expression levels with regard to clinicopathological factors including postoperative prognosis in 167 patients with BC. Expression of RFX1 was heterogeneous among cell lines, and we found no DNA methylation at the RFX1 promoter region. Patients were categorized into groups with high or low RFX1 expression, based on ratio of RFX1 mRNA expression in BC and adjacent non‑cancerous tissues. The high RFX1 group was significantly associated with low T factor (P=0.028), earlier disease stage (P=0.015), positive expression of estrogen receptor (P=0.005) and progesterone receptor (P=0.011), negative expression of human epidermal growth factor receptor 2 (P=0.001). The high RFX1 group experienced more favorable disease‑free survival (P=0.007) and overall survival (P=0.013). In multivariate analysis, RFX1 expression was an independent prognostic factor for disease‑free survival. Our findings indicate that RFX1 may serve as a prognostic marker for BC.
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1 

Jemal A, Center MM, DeSantis C and Ward EM: Global patterns of cancer incidence and mortality rates and trends. Cancer Epidemiol Biomarkers Prev. 19:1893–1907. 2010. View Article : Google Scholar : PubMed/NCBI

2 

Torre LA, Bray F, Siegel RL, Ferlay J, Lortet-Tieulent J and Jemal A: Global cancer statistics, 2012. CA Cancer J Clin. 65:87–108. 2015. View Article : Google Scholar : PubMed/NCBI

3 

Coates AS, Winer EP, Goldhirsch A, Gelber RD, Gnant M, Piccart-Gebhart M, Thürlimann B and Senn HJ: Panel Members: Tailoring therapies-improving the management of early breast cancer: St Gallen international expert consensus on the primary therapy of early breast cancer 2015. Ann Oncol. 26:1533–1546. 2015. View Article : Google Scholar : PubMed/NCBI

4 

Emery P, Durand B, Mach B and Reith W: RFX proteins, a novel family of DNA binding proteins conserved in the eukaryotic kingdom. Nucleic Acids Res. 24:803–807. 1996. View Article : Google Scholar : PubMed/NCBI

5 

Ohashi Y, Ueda M, Kawase T, Kawakami Y and Toda M: Identification of an epigenetically silenced gene, RFX1, in human glioma cells using restriction landmark genomic scanning. Oncogene. 23:7772–7779. 2004. View Article : Google Scholar : PubMed/NCBI

6 

Feng C, Zhang Y, Yin J, Li J, Abounader R and Zuo Z: Regulatory factor X1 is a new tumor suppressive transcription factor that acts via direct downregulation of CD44 in glioblastoma. Neuro Oncol. 16:1078–1085. 2014. View Article : Google Scholar : PubMed/NCBI

7 

Su JC, Tseng PH, Hsu CY, Tai WT, Huang JW, Ko CH, Lin MW, Liu CY, Chen KF and Shiau CW: RFX1-dependent activation of SHP-1 induces autophagy by a novel obatoclax derivative in hepatocellular carcinoma cells. Oncotarget. 5:4909–4919. 2014. View Article : Google Scholar : PubMed/NCBI

8 

Chen L, Smith L, Johnson MR, Wang K, Diasio RB and Smith JB: Activation of protein kinase C induces nuclear translocation of RFX1 and down-regulates c-myc via an intron 1 X box in undifferentiated leukemia HL-60 cells. J Biol Chem. 275:32227–32233. 2000. View Article : Google Scholar : PubMed/NCBI

9 

Kanda M, Shimizu D, Fujii T, Tanaka H, Shibata M, Iwata N, Hayashi M, Kobayashi D, Tanaka C, Yamada S, et al: Protein arginine methyltransferase 5 is associated with malignant phenotype and peritoneal metastasis in gastric cancer. Int J Oncol. 49:1195–1202. 2016.PubMed/NCBI

10 

Kanda M, Shimizu D, Nomoto S, Takami H, Hibino S, Oya H, Hashimoto R, Suenaga M, Inokawa Y, Kobayashi D, et al: Prognostic impact of expression and methylation status of DENN/MADD domain-containing protein 2D in gastric cancer. Gastric Cancer. 18:288–296. 2015. View Article : Google Scholar : PubMed/NCBI

11 

Kanda M, Nomoto S, Oya H, Takami H, Shimizu D, Hibino S, Hashimoto R, Kobayashi D, Tanaka C, Yamada S, et al: The expression of melanoma-associated antigen D2 both in surgically resected and serum samples serves as clinically relevant biomarker of gastric cancer progression. Ann Surg Oncol. 23:(Suppl 2). S214–S221. 2016. View Article : Google Scholar : PubMed/NCBI

12 

Kanda M, Shimizu D, Fujii T, Sueoka S, Tanaka Y, Ezaka K, Takami H, Tanaka H, Hashimoto R, Iwata N, et al: Function and diagnostic value of Anosmin-1 in gastric cancer progression. Int J Cancer. 138:721–730. 2016. View Article : Google Scholar : PubMed/NCBI

13 

Takai D and Jones PA: Comprehensive analysis of CpG islands in human chromosomes 21 and 22. Proc Natl Acad Sci USA. 99:3740–3745. 2002. View Article : Google Scholar : PubMed/NCBI

14 

Li LC and Dahiya R: MethPrimer: Designing primers for methylation PCRs. Bioinformatics. 18:1427–1431. 2002. View Article : Google Scholar : PubMed/NCBI

15 

Kanda M, Shimizu D, Tanaka H, Shibata M, Iwata N, Hayashi M, Kobayashi D, Tanaka C, Yamada S, Fujii T, et al: Metastatic pathway-specific transcriptome analysis identifies MFSD4 as a putative tumor suppressor and biomarker for hepatic metastasis in patients with gastric cancer. Oncotarget. 7:13667–13679. 2016.PubMed/NCBI

16 

Finn RS, Dering J, Conklin D, Kalous O, Cohen DJ, Desai AJ, Ginther C, Atefi M, Chen I, Fowst C, et al: PD 0332991, a selective cyclin D kinase 4/6 inhibitor, preferentially inhibits proliferation of luminal estrogen receptor-positive human breast cancer cell lines in vitro. Breast Cancer Res. 11:R772009. View Article : Google Scholar : PubMed/NCBI

17 

Subik K, Lee JF, Baxter L, Strzepek T, Costello D, Crowley P, Xing L, Hung MC, Bonfiglio T, Hicks DG and Tang P: The expression patterns of ER, PR, HER2, CK5/6, EGFR, Ki-67 and AR by immunohistochemical analysis in breast cancer cell lines. Breast Cancer (Auckl). 4:35–41. 2010.PubMed/NCBI

18 

Gajiwala KS, Chen H, Cornille F, Roques BP, Reith W, Mach B and Burley SK: Structure of the winged-helix protein hRFX1 reveals a new mode of DNA binding. Nature. 403:916–921. 2000. View Article : Google Scholar : PubMed/NCBI

19 

Reith W, Ucla C, Barras E, Gaud A, Durand B, Herrero-Sanchez C, Kobr M and Mach B: Rfx1, A transactivator of hepatitis-B virus enhancer-I, belongs to a novel family of homodimeric and heterodimeric DNA-binding proteins. Mol Cell Biol. 14:1230–1244. 1994. View Article : Google Scholar : PubMed/NCBI

20 

Katan-Khaykovich Y, Spiegel I and Shaul Y: The dimerization/repression domain of RFX1 is related to a conserved region of its yeast homologues Crt1 and Sak1: A new function for an ancient motif. J Mol Biol. 294:121–137. 1999. View Article : Google Scholar : PubMed/NCBI

21 

Sengupta PK, Smith EM, Kim K, Murnane MJ and Smith BD: DNA hypermethylation near the transcription start site of collagen alpha2(I) gene occurs in both cancer cell lines and primary colorectal cancers. Cancer Res. 63:1789–1797. 2003.PubMed/NCBI

22 

Watts JA, Zhang C, Klein-Szanto AJ, Kormish JD, Fu J, Zhang MQ and Zaret KS: Study of FoxA pioneer factor at silent genes reveals Rfx-repressed enhancer at Cdx2 and a potential indicator of esophageal adenocarcinoma development. PLoS Genet. 7:e10022772011. View Article : Google Scholar : PubMed/NCBI

23 

Rauscher GH, Kresovich JK, Poulin M, Yan L, Macias V, Mahmoud AM, Al-Alem U, Kajdacsy-Balla A, Wiley EL, Tonetti D and Ehrlich M: Exploring DNA methylation changes in promoter, intragenic, and intergenic regions as early and late events in breast cancer formation. BMC Cancer. 15:8162015. View Article : Google Scholar : PubMed/NCBI

24 

Yang X, Han H, De Carvalho DD, Lay FD, Jones PA and Liang G: Gene body methylation can alter gene expression and is a therapeutic target in cancer. Cancer Cell. 26:577–590. 2014. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Shibata M, Kanda M, Shimizu D, Tanaka H, Umeda S, Hayashi M, Inaishi T, Miyajima N, Adachi Y, Takano Y, Takano Y, et al: Expression of regulatory factor X1 can predict the prognosis of breast cancer. Oncol Lett 13: 4334-4340, 2017.
APA
Shibata, M., Kanda, M., Shimizu, D., Tanaka, H., Umeda, S., Hayashi, M. ... Kikumori, T. (2017). Expression of regulatory factor X1 can predict the prognosis of breast cancer. Oncology Letters, 13, 4334-4340. https://doi.org/10.3892/ol.2017.6005
MLA
Shibata, M., Kanda, M., Shimizu, D., Tanaka, H., Umeda, S., Hayashi, M., Inaishi, T., Miyajima, N., Adachi, Y., Takano, Y., Nakanishi, K., Takeuchi, D., Noda, S., Kodera, Y., Kikumori, T."Expression of regulatory factor X1 can predict the prognosis of breast cancer". Oncology Letters 13.6 (2017): 4334-4340.
Chicago
Shibata, M., Kanda, M., Shimizu, D., Tanaka, H., Umeda, S., Hayashi, M., Inaishi, T., Miyajima, N., Adachi, Y., Takano, Y., Nakanishi, K., Takeuchi, D., Noda, S., Kodera, Y., Kikumori, T."Expression of regulatory factor X1 can predict the prognosis of breast cancer". Oncology Letters 13, no. 6 (2017): 4334-4340. https://doi.org/10.3892/ol.2017.6005
Copy and paste a formatted citation
x
Spandidos Publications style
Shibata M, Kanda M, Shimizu D, Tanaka H, Umeda S, Hayashi M, Inaishi T, Miyajima N, Adachi Y, Takano Y, Takano Y, et al: Expression of regulatory factor X1 can predict the prognosis of breast cancer. Oncol Lett 13: 4334-4340, 2017.
APA
Shibata, M., Kanda, M., Shimizu, D., Tanaka, H., Umeda, S., Hayashi, M. ... Kikumori, T. (2017). Expression of regulatory factor X1 can predict the prognosis of breast cancer. Oncology Letters, 13, 4334-4340. https://doi.org/10.3892/ol.2017.6005
MLA
Shibata, M., Kanda, M., Shimizu, D., Tanaka, H., Umeda, S., Hayashi, M., Inaishi, T., Miyajima, N., Adachi, Y., Takano, Y., Nakanishi, K., Takeuchi, D., Noda, S., Kodera, Y., Kikumori, T."Expression of regulatory factor X1 can predict the prognosis of breast cancer". Oncology Letters 13.6 (2017): 4334-4340.
Chicago
Shibata, M., Kanda, M., Shimizu, D., Tanaka, H., Umeda, S., Hayashi, M., Inaishi, T., Miyajima, N., Adachi, Y., Takano, Y., Nakanishi, K., Takeuchi, D., Noda, S., Kodera, Y., Kikumori, T."Expression of regulatory factor X1 can predict the prognosis of breast cancer". Oncology Letters 13, no. 6 (2017): 4334-4340. https://doi.org/10.3892/ol.2017.6005
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