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Article

Oxymatrine inhibits proliferation of human bladder cancer T24 cells by inducing apoptosis and cell cycle arrest

  • Authors:
    • Shun Li
    • Yi Zhang
    • Qingyong Liu
    • Qingli Zhao
    • Liuyu Xu
    • Shengliang Huang
    • Shiming Huang
    • Xuebin Wei
  • View Affiliations / Copyright

    Affiliations: Department of Urology, Qianfoshan Hospital of Shandong University, Jinan, Shandong 250013, P.R. China, Department of Radiology, The First People's Hospital of Jinan, Jinan, Shandong 250000, P.R. China
  • Pages: 4453-4458
    |
    Published online on: April 7, 2017
       https://doi.org/10.3892/ol.2017.6013
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Abstract

Oxymatrine has been shown to exert an antitumor effect on several types of cancer cells. However, the role of oxymatrine in bladder cancer has not yet been evaluated. The present study was designed to investigate the potential anti‑proliferative effect of oxymatrine on bladder cancer T24 cells and the possible mechanisms involved. A 3‑(4,5‑dimethylthiazol‑2‑yl)‑2,5‑diphenyltetrazolium bromide assay was used to determine cell growth, and the cell morphology was examined using hematoxylin and eosin staining, wrights' staining and electron microscopy. The caspase‑3 and survivin mRNA and protein levels were assessed using reverse transcription‑quantitative polymerase chain reaction and western blot analysis, respectively. The expression of tumor protein p53 (p53), Bcl‑2‑associated X protein (Bax) and B‑cell lymphoma 2 (Bcl‑2) were analyzed using immunohistochemistry. Oxymatrine inhibited the proliferation of the T24 cells in a dose‑ and time‑dependent manner. Oxymatrine also induced apoptosis and cell cycle arrest in the cells, in association with the upregulation of caspase‑3 and Bax, and the downregulation of survivin, Bcl‑2 and p53 expression. Overall, oxymatrine inhibits the proliferation of human bladder cancer cells by inducing apoptosis and cell cycle arrest via mechanisms that involve p53‑Bax signaling and the downregulation of survivin expression.
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Copy and paste a formatted citation
Spandidos Publications style
Li S, Zhang Y, Liu Q, Zhao Q, Xu L, Huang S, Huang S and Wei X: Oxymatrine inhibits proliferation of human bladder cancer T24 cells by inducing apoptosis and cell cycle arrest. Oncol Lett 13: 4453-4458, 2017.
APA
Li, S., Zhang, Y., Liu, Q., Zhao, Q., Xu, L., Huang, S. ... Wei, X. (2017). Oxymatrine inhibits proliferation of human bladder cancer T24 cells by inducing apoptosis and cell cycle arrest. Oncology Letters, 13, 4453-4458. https://doi.org/10.3892/ol.2017.6013
MLA
Li, S., Zhang, Y., Liu, Q., Zhao, Q., Xu, L., Huang, S., Huang, S., Wei, X."Oxymatrine inhibits proliferation of human bladder cancer T24 cells by inducing apoptosis and cell cycle arrest". Oncology Letters 13.6 (2017): 4453-4458.
Chicago
Li, S., Zhang, Y., Liu, Q., Zhao, Q., Xu, L., Huang, S., Huang, S., Wei, X."Oxymatrine inhibits proliferation of human bladder cancer T24 cells by inducing apoptosis and cell cycle arrest". Oncology Letters 13, no. 6 (2017): 4453-4458. https://doi.org/10.3892/ol.2017.6013
Copy and paste a formatted citation
x
Spandidos Publications style
Li S, Zhang Y, Liu Q, Zhao Q, Xu L, Huang S, Huang S and Wei X: Oxymatrine inhibits proliferation of human bladder cancer T24 cells by inducing apoptosis and cell cycle arrest. Oncol Lett 13: 4453-4458, 2017.
APA
Li, S., Zhang, Y., Liu, Q., Zhao, Q., Xu, L., Huang, S. ... Wei, X. (2017). Oxymatrine inhibits proliferation of human bladder cancer T24 cells by inducing apoptosis and cell cycle arrest. Oncology Letters, 13, 4453-4458. https://doi.org/10.3892/ol.2017.6013
MLA
Li, S., Zhang, Y., Liu, Q., Zhao, Q., Xu, L., Huang, S., Huang, S., Wei, X."Oxymatrine inhibits proliferation of human bladder cancer T24 cells by inducing apoptosis and cell cycle arrest". Oncology Letters 13.6 (2017): 4453-4458.
Chicago
Li, S., Zhang, Y., Liu, Q., Zhao, Q., Xu, L., Huang, S., Huang, S., Wei, X."Oxymatrine inhibits proliferation of human bladder cancer T24 cells by inducing apoptosis and cell cycle arrest". Oncology Letters 13, no. 6 (2017): 4453-4458. https://doi.org/10.3892/ol.2017.6013
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