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Article Open Access

Relationship between DLK1 gene promoter region DNA methylation and non-small cell lung cancer biological behavior

Retraction in: /10.3892/ol.2020.11577
  • Authors:
    • Zhaokui Zhong
    • Yongting Ye
    • Wei Guo
    • Yi He
    • Weicai Hu
  • View Affiliations / Copyright

    Affiliations: Department of Thoracic Surgery, Central Hospital of Zhumadian, Zhumadian, Henan 463000, P.R. China, Department of Internal Medicine, The Fourth People's Hospital of Zhumadian, Zhumadian, Henan 463000, P.R. China, Department of Thoracic Surgery, Henan Provincial People's Hospital, Zhengzhou, Henan 450003, P.R. China
    Copyright: © Zhong et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 4123-4126
    |
    Published online on: April 10, 2017
       https://doi.org/10.3892/ol.2017.6019
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Abstract

We investigated the possible association between DLKI gene promoter region methylation and the increased invasion capacity of non-small cell lung cancer (NSCLC). Lung cancer cell line H1299, as well as the gene transfection and RNA interference technology were used to build DLK gene overexpression and knockdown cells. An in vitro invasion assay was performed to observe the changes in the invasion ability of lung cancer cells. Western blot analysis was used to verify Notchl and matrix metalloproteinase-9 (MMP-9) expression levels and a sulfurous acid sequencing technique was used to test the DNA methylation level in the promoter region. Our results showed that the invasion ability of cells in the overexpression group was significantly enhanced. This ability was considerably reduced in the knockdown group. The Notchl and MMP-9 expression level increased significantly in the overexpression group, while it was reduced considerably in the knockdown group. We detected significantly lower levels of DNA methylation in the promoter region in the over­expression group. It was concluded that methylation of the DLK1 gene promoter region increased the invasion ability of NSCLC. Furthermore, it is possible that this process is related to the Notch signaling pathway.
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Copy and paste a formatted citation
Spandidos Publications style
Zhong Z, Ye Y, Guo W, He Y and Hu W: Relationship between DLK1 gene promoter region DNA methylation and non-small cell lung cancer biological behavior Retraction in /10.3892/ol.2020.11577. Oncol Lett 13: 4123-4126, 2017.
APA
Zhong, Z., Ye, Y., Guo, W., He, Y., & Hu, W. (2017). Relationship between DLK1 gene promoter region DNA methylation and non-small cell lung cancer biological behavior Retraction in /10.3892/ol.2020.11577. Oncology Letters, 13, 4123-4126. https://doi.org/10.3892/ol.2017.6019
MLA
Zhong, Z., Ye, Y., Guo, W., He, Y., Hu, W."Relationship between DLK1 gene promoter region DNA methylation and non-small cell lung cancer biological behavior Retraction in /10.3892/ol.2020.11577". Oncology Letters 13.6 (2017): 4123-4126.
Chicago
Zhong, Z., Ye, Y., Guo, W., He, Y., Hu, W."Relationship between DLK1 gene promoter region DNA methylation and non-small cell lung cancer biological behavior Retraction in /10.3892/ol.2020.11577". Oncology Letters 13, no. 6 (2017): 4123-4126. https://doi.org/10.3892/ol.2017.6019
Copy and paste a formatted citation
x
Spandidos Publications style
Zhong Z, Ye Y, Guo W, He Y and Hu W: Relationship between DLK1 gene promoter region DNA methylation and non-small cell lung cancer biological behavior Retraction in /10.3892/ol.2020.11577. Oncol Lett 13: 4123-4126, 2017.
APA
Zhong, Z., Ye, Y., Guo, W., He, Y., & Hu, W. (2017). Relationship between DLK1 gene promoter region DNA methylation and non-small cell lung cancer biological behavior Retraction in /10.3892/ol.2020.11577. Oncology Letters, 13, 4123-4126. https://doi.org/10.3892/ol.2017.6019
MLA
Zhong, Z., Ye, Y., Guo, W., He, Y., Hu, W."Relationship between DLK1 gene promoter region DNA methylation and non-small cell lung cancer biological behavior Retraction in /10.3892/ol.2020.11577". Oncology Letters 13.6 (2017): 4123-4126.
Chicago
Zhong, Z., Ye, Y., Guo, W., He, Y., Hu, W."Relationship between DLK1 gene promoter region DNA methylation and non-small cell lung cancer biological behavior Retraction in /10.3892/ol.2020.11577". Oncology Letters 13, no. 6 (2017): 4123-4126. https://doi.org/10.3892/ol.2017.6019
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