Knockout of krüppel-like factor 10 suppresses hepatic cell proliferation in a partially hepatectomized mouse model

  • Authors:
    • Seung‑Ho Heo
    • Eui‑Suk Jeong
    • Kyoung‑Sun Lee
    • Jin‑Hee Seo
    • Woon‑Kyu Lee
    • Yang‑Kyu Choi
  • View Affiliations

  • Published online on: April 19, 2017     https://doi.org/10.3892/ol.2017.6044
  • Pages: 4843-4848
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Abstract

The liver has marked regenerative capabilities, and numerous signaling pathways are involved in liver regeneration. The transforming growth factor‑β (TGF-β)/Smad pathway, which is also involved in liver regeneration, regulates numerous biological processes. Krüppel-like factor 10 (KLF10) has been reported to activate the TGF‑β/Smad signaling pathway; however, the exact functions of KLF10 under various pathophysiological conditions remain unclear. In the present study, the role of KLF10 in liver regeneration following partial hepatectomy (PH) was investigated using KLF10‑knockout (KO) mice. KLF10‑KO mice exhibited lower liver/body weight ratios and 5‑bromo‑2‑deoxy‑uridine labeling indices compared with wild‑type (WT) mice, and significant differences (P=0.028) were obtained at 72 h after PH. To understand the causes of the gross and histopathological findings, the expression levels of the components of the TGF‑β/Smad pathway were examined using reverse transcription‑quantitative polymerase chain reaction and western blot analysis. The mRNA and protein levels of Smad3, p15, TGF‑β1 and TGF‑β receptor 1 were significantly increased, while those of cMyc and cyclin D1 (proliferation‑associated genes) were significantly lower in the liver tissues of the KLF10‑KO mice compared with those of the WT mice at 72 h post‑PH. These results indicated that KLF10‑KO may exhibit antiproliferative effects on liver regeneration following PH, through strengthening the TGF‑β/Smad signaling pathway in a delayed manner.
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June-2017
Volume 13 Issue 6

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Heo SH, Jeong ES, Lee KS, Seo JH, Lee WK and Choi YK: Knockout of krüppel-like factor 10 suppresses hepatic cell proliferation in a partially hepatectomized mouse model. Oncol Lett 13: 4843-4848, 2017
APA
Heo, S., Jeong, E., Lee, K., Seo, J., Lee, W., & Choi, Y. (2017). Knockout of krüppel-like factor 10 suppresses hepatic cell proliferation in a partially hepatectomized mouse model. Oncology Letters, 13, 4843-4848. https://doi.org/10.3892/ol.2017.6044
MLA
Heo, S., Jeong, E., Lee, K., Seo, J., Lee, W., Choi, Y."Knockout of krüppel-like factor 10 suppresses hepatic cell proliferation in a partially hepatectomized mouse model". Oncology Letters 13.6 (2017): 4843-4848.
Chicago
Heo, S., Jeong, E., Lee, K., Seo, J., Lee, W., Choi, Y."Knockout of krüppel-like factor 10 suppresses hepatic cell proliferation in a partially hepatectomized mouse model". Oncology Letters 13, no. 6 (2017): 4843-4848. https://doi.org/10.3892/ol.2017.6044