Maslinic acid suppresses the growth of human gastric cells by inducing apoptosis via inhibition of the interleukin-6 mediated Janus kinase/signal transducer and activator of transcription 3 signaling pathway

  • Authors:
    • Deyou Wang
    • Sifeng Tang
    • Qiwen Zhang
  • View Affiliations

  • Published online on: April 21, 2017     https://doi.org/10.3892/ol.2017.6073
  • Pages: 4875-4881
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Abstract

The present study aimed to determine whether maslinic acid effectively inhibits the proliferation of MKN28 cells, and to investigate the mechanisms underlying its antitumor functions. MKN28 cell viability was evaluated using a Cell Counting Kit-8, cell proliferation was analyzed by a colony formation assay and flow cytometry was used to investigate the rate of apoptosis. Western blot analysis was performed in order to determine the differential expression levels of Janus kinase (JAK), signal transducer and activator of transcription 3 (STAT3) and apoptosis associated proteins B‑cell lymphoma 2 (Bcl‑2), Bcl‑2 associated X protein (Bax) and Bcl‑2 associated agonist of cell death (Bad). Interleukin‑6 (IL‑6) concentration was evaluated using ELISA. IL‑6 and anti‑IL‑6 antibodies were used to investigate the role of IL‑6 in MKN28 cells treated with maslinic acid proliferation, and the STAT3 phosphorylation rates. The results demonstrated that maslinic acid treatment significantly reduced cell proliferation, induced apoptosis and was accompanied by a significant decrease in Bcl‑2, Bax and Bad expression levels. Maslinic acid treatment also resulted in the downregulation of phosphorylated‑STAT3 and JAK2, and significantly inhibited the protein expression of IL‑6. Maslinic acid is able to inhibit MKN28 cell proliferation and the phosphorylation of STAT3 by downregulating the expression of IL‑6. These results suggest that maslinic acid suppresses the growth of MKN28 cells by inducing apoptosis via its inhibition of the IL‑6/JAK/STAT3 signaling cascade.
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June-2017
Volume 13 Issue 6

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Wang D, Tang S and Zhang Q: Maslinic acid suppresses the growth of human gastric cells by inducing apoptosis via inhibition of the interleukin-6 mediated Janus kinase/signal transducer and activator of transcription 3 signaling pathway. Oncol Lett 13: 4875-4881, 2017
APA
Wang, D., Tang, S., & Zhang, Q. (2017). Maslinic acid suppresses the growth of human gastric cells by inducing apoptosis via inhibition of the interleukin-6 mediated Janus kinase/signal transducer and activator of transcription 3 signaling pathway. Oncology Letters, 13, 4875-4881. https://doi.org/10.3892/ol.2017.6073
MLA
Wang, D., Tang, S., Zhang, Q."Maslinic acid suppresses the growth of human gastric cells by inducing apoptosis via inhibition of the interleukin-6 mediated Janus kinase/signal transducer and activator of transcription 3 signaling pathway". Oncology Letters 13.6 (2017): 4875-4881.
Chicago
Wang, D., Tang, S., Zhang, Q."Maslinic acid suppresses the growth of human gastric cells by inducing apoptosis via inhibition of the interleukin-6 mediated Janus kinase/signal transducer and activator of transcription 3 signaling pathway". Oncology Letters 13, no. 6 (2017): 4875-4881. https://doi.org/10.3892/ol.2017.6073