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Article Open Access

MicroRNA-375 suppresses esophageal cancer cell growth and invasion by repressing metadherin expression

  • Authors:
    • Changmei Hu
    • Liang Lv
    • Jie Peng
    • Deliang Liu
    • Xuehong Wang
    • Yuqian Zhou
    • Jirong Huo
  • View Affiliations / Copyright

    Affiliations: Department of Gastroenterology, The Second Xiangya Hospital of Central South University, Changsha, Hunan 410011, P.R. China, Department of Haematology, Xiangya Hospital of Central South University, Changsha, Hunan 410078, P.R. China
    Copyright: © Hu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 4769-4775
    |
    Published online on: April 26, 2017
       https://doi.org/10.3892/ol.2017.6098
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Abstract

Accumulating evidence indicates that aberrant expression of microRNAs is involved in tumorigenesis, tumor progression and response to therapy. MicroRNA-375 (miR-375) is an important cancer‑associated RNA that is downregulated in multiple types of cancer. In the present study, the potential effects of and underlying molecular mechanism for miR‑375 in esophageal cancer were investigated. The expression of miR‑375 in paired esophageal squamous cell carcinoma (ESCC) and non‑tumor tissues from 10 patients was quantified using the reverse transcription‑quantitative polymerase chain reaction. The miR‑375 levels in the ESCC cell line EC109 and a normal esophageal epithelial cell line, Het‑1A, were also detected. The effect of miR‑375 on ESCC cell growth and invasion was determined using Cell Counting kit‑8, flow cytometry and invasion assays. A luciferase assay was conducted for target identification. The results of the present study revealed that miR‑375 was downregulated in ESCC tumor tissue and EC109 cells compared with normal tissue and Het‑1A cells (P<0.01). Overexpression of miR‑375 inhibited EC109 cell growth and invasion, and induced cell cycle arrest. In addition, metadherin (MTDH) was demonstrated to be a direct target of miR‑375 (P<0.01). The overexpression of miR‑375 downregulated MTDH (P<0.01), cyclin D1 (P<0.05) and vascular endothelial growth factor (P<0.01) expression, while upregulating epithelial cadherin (P<0.01) expression, which may account for its effect on ESCC cell proliferation and invasion. The results of the present study suggest that the miR‑375/MTDH axis represents a target for the treatment of ESCC.
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1 

Kamangar F, Qiao YL, Schiller JT, Dawsey SM, Fears T, Sun XD, Abnet CC, Zhao P, Taylor PR and Mark SD: Human papillomavirus serology and the risk of esophageal and gastric cancers: Results from a cohort in a high-risk region in China. Int J Cancer. 119:579–584. 2006. View Article : Google Scholar : PubMed/NCBI

2 

Arnold M, Soerjomataram I, Ferlay J and Forman D: Global incidence of oesophageal cancer by histological subtype in 2012. Gut. 64:381–387. 2015. View Article : Google Scholar : PubMed/NCBI

3 

Lagergren J: Oesophageal cancer in 2014: Advances in curatively intended treatment. Nat Rev Gastroenterol Hepatol. 12:74–75. 2015. View Article : Google Scholar : PubMed/NCBI

4 

Guo Y, Chen Z, Zhang L, Zhou F, Shi S, Feng X, Li B, Meng X, Ma X, Luo M, et al: Distinctive microRNA profiles relating to patient survival in esophageal squamous cell carcinoma. Cancer Res. 68:26–33. 2008. View Article : Google Scholar : PubMed/NCBI

5 

He L and Hannon GJ: MicroRNAs: Small RNAs with a big role in gene regulation. Nat Rev Genet. 5:522–531. 2004. View Article : Google Scholar : PubMed/NCBI

6 

Chen K and Rajewsky N: The evolution of gene regulation by transcription factors and microRNAs. Nat Rev Genet. 8:93–103. 2007. View Article : Google Scholar : PubMed/NCBI

7 

Fendler A, Jung M, Stephan C, Honey RJ, Stewart RJ, Pace KT, Erbersdobler A, Samaan S, Jung K and Yousef GM: miRNAs can predict prostate cancer biochemical relapse and are involved in tumor progression. Int J Oncol. 39:1183–1192. 2011.PubMed/NCBI

8 

Poy MN, Eliasson L, Krutzfeldt J, Kuwajima S, Ma X, Macdonald PE, Pfeffer S, Tuschl T, Rajewsky N, Rorsman P and Stoffel M: A pancreatic islet-specific microRNA regulates insulin secretion. Nature. 432:226–230. 2004. View Article : Google Scholar : PubMed/NCBI

9 

van de Bunt M, Gaulton KJ, Parts L, Moran I, Johnson PR, Lindgren CM, Ferrer J, Gloyn AL and McCarthy MI: The miRNA profile of human pancreatic islets and beta-cells and relationship to type 2 diabetes pathogenesis. PLoS One. 8:e552722013. View Article : Google Scholar : PubMed/NCBI

10 

Yan JW, Lin JS and He XX: The emerging role of miR-375 in cancer. Int J Cancer. 135:1011–1018. 2014. View Article : Google Scholar : PubMed/NCBI

11 

Wang F, Li Y, Zhou J, Xu J, Peng C, Ye F, Shen Y, Lu W, Wan X and Xie X: miR-375 is down-regulated in squamous cervical cancer and inhibits cell migration and invasion via targeting transcription factor SP1. Am J Pathol. 179:2580–2588. 2011. View Article : Google Scholar : PubMed/NCBI

12 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(−Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

13 

Agarwal V, Bell GW, Nam JW and Bartel DP: Predicting effective microRNA target sites in mammalian mRNAs. Elife. 4:2015. View Article : Google Scholar

14 

Li D, Li X, Cao W, Qi Y and Yang X: Antagonism of microRNA-99a promotes cell invasion and down-regulates E-cadherin expression in pancreatic cancer cells by regulating mammalian target of rapamycin. Acta Histochem. 116:723–729. 2014. View Article : Google Scholar : PubMed/NCBI

15 

Bartels CL and Tsongalis GJ: MicroRNAs: Novel biomarkers for human cancer. Clin Chem. 55:623–631. 2009. View Article : Google Scholar : PubMed/NCBI

16 

Komatsu S, Ichikawa D, Takeshita H, Tsujiura M, Morimura R, Nagata H, Kosuga T, Iitaka D, Konishi H, Shiozaki A, et al: Circulating microRNAs in plasma of patients with oesophageal squamous cell carcinoma. Br J Cancer. 105:104–111. 2011. View Article : Google Scholar : PubMed/NCBI

17 

Yan S, Jiang H, Fang S, Yin F, Wang Z, Jia Y, Sun X, Wu S, Jiang T and Mao A: MicroRNA-340 inhibits esophageal cancer cell growth and invasion by targeting phosphoserine aminotransferase 1. Cell Physiol Biochem. 37:375–386. 2015. View Article : Google Scholar : PubMed/NCBI

18 

Sun Y, Yu X and Bai Q: miR-204 inhibits invasion and epithelial-mesenchymal transition by targeting FOXM1 in esophageal cancer. Int J Clin Exp Pathol. 8:12775–12783. 2015.PubMed/NCBI

19 

Komatsu S, Ichikawa D, Takeshita H, Konishi H, Nagata H, Hirajima S, Kawaguchi T, Arita T, Shiozaki A, Fujiwara H, et al: Prognostic impact of circulating miR-21 and miR-375 in plasma of patients with esophageal squamous cell carcinoma. Expert Opin Biol Ther. 12 Suppl 1:S53–S59. 2012. View Article : Google Scholar : PubMed/NCBI

20 

He XX, Chang Y, Meng FY, Wang MY, Xie QH, Tang F, Li PY, Song YH and Lin JS: MicroRNA-375 targets AEG-1 in hepatocellular carcinoma and suppresses liver cancer cell growth in vitro and in vivo. Oncogene. 31:3357–3369. 2012. View Article : Google Scholar : PubMed/NCBI

21 

Mathé EA, Nguyen GH, Bowman ED, Zhao Y, Budhu A, Schetter AJ, Braun R, Reimers M, Kumamoto K, Hughes D, et al: MicroRNA expression in squamous cell carcinoma and adenocarcinoma of the esophagus: Associations with survival. Clin Cancer Res. 15:6192–6200. 2009. View Article : Google Scholar : PubMed/NCBI

22 

Kano M, Seki N, Kikkawa N, Fujimura L, Hoshino I, Akutsu Y, Chiyomaru T, Enokida H, Nakagawa M and Matsubara H: miR-145, miR-133a and miR-133b: Tumor-suppressive miRNAs target FSCN1 in esophageal squamous cell carcinoma. Int J Cancer. 127:2804–2814. 2010. View Article : Google Scholar : PubMed/NCBI

23 

Kang DC, Su ZZ, Sarkar D, Emdad L, Volsky DJ and Fisher PB: Cloning and characterization of HIV-1-inducible astrocyte elevated gene-1, AEG-1. Gene. 353:8–15. 2005. View Article : Google Scholar : PubMed/NCBI

24 

Brown DM and Ruoslahti E: Metadherin, a cell surface protein in breast tumors that mediates lung metastasis. Cancer Cell. 5:365–374. 2004. View Article : Google Scholar : PubMed/NCBI

25 

Li WF, Ou Q, Dai H and Liu CA: Lentiviral-mediated short hairpin RNA knockdown of MTDH inhibits cell growth and induces apoptosis by regulating the PTEN/AKT pathway in hepatocellular carcinoma. Int J Mol Sci. 16:19419–19432. 2015. View Article : Google Scholar : PubMed/NCBI

26 

Yang C, Zheng S, Liu Q, Liu T, Lu M, Dai F, Gao X, Sheyhidin I and Lu X: Metadherin is required for the proliferation, migration, and invasion of esophageal squamous cell carcinoma and its meta-analysis. Transl Res. 166:614–626.e2. 2015. View Article : Google Scholar : PubMed/NCBI

27 

Holash J, Maisonpierre PC, Compton D, Boland P, Alexander CR, Zagzag D, Yancopoulos GD and Wiegand SJ: Vessel cooption, regression, and growth in tumors mediated by angiopoietins and VEGF. Science. 284:1994–1998. 1999. View Article : Google Scholar : PubMed/NCBI

28 

Eriksson A, Cao R, Pawliuk R, Berg SM, Tsang M, Zhou D, Fleet C, Tritsaris K, Dissing S, Leboulch P and Cao Y: Placenta growth factor-1 antagonizes VEGF-C-induced angiogenesis and tumor growth by the formation of functionally inactive PlGF-1/VEGF heterodimers. Cancer Cell. 1:99–108. 2002. View Article : Google Scholar : PubMed/NCBI

29 

Zhu GC, Yu CY, She L, Tan HL, Li G, Ren SL, Su ZW, Wei M, Huang DH, Tian YQ, et al: Metadherin regulation of vascular endothelial growth factor expression is dependent upon the PI3K/Akt pathway in squamous cell carcinoma of the head and neck. Medicine (Baltimore). 94:e5022015. View Article : Google Scholar : PubMed/NCBI

30 

Emdad L, Lee SG, Su ZZ, Jeon HY, Boukerche H, Sarkar D and Fisher PB: Astrocyte elevated gene-1 (AEG-1) functions as an oncogene and regulates angiogenesis. Proc Natl Acad Sci USA. 106:pp. 21300–21305. 2009; View Article : Google Scholar : PubMed/NCBI

31 

Fu M, Wang C, Li Z, Sakamaki T and Pestell RG: Minireview: Cyclin D1: Normal and abnormal functions. Endocrinology. 145:5439–5447. 2004. View Article : Google Scholar : PubMed/NCBI

32 

Li PP, Feng LL, Chen N, Ge XL, Lv X, Lu K, Ding M, Yuan D and Wang X: Metadherin contributes to the pathogenesis of chronic lymphocytic leukemia partially through Wnt/β-catenin pathway. Med Oncol. 32:4792015. View Article : Google Scholar : PubMed/NCBI

33 

Cano A, Pérez-Moreno MA, Rodrigo I, Locascio A, Blanco MJ, Del Barrio MG, Portillo F and Nieto MA: The transcription factor snail controls epithelial-mesenchymal transitions by repressing E-cadherin expression. Nat Cell Biol. 2:76–83. 2000. View Article : Google Scholar : PubMed/NCBI

34 

Li X, Kong X, Huo Q, Guo H, Yan S, Yuan C, Moran MS, Shao C and Yang Q: Metadherin enhances the invasiveness of breast cancer cells by inducing epithelial to mesenchymal transition. Cancer Sci. 102:1151–1157. 2011. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Hu C, Lv L, Peng J, Liu D, Wang X, Zhou Y and Huo J: MicroRNA-375 suppresses esophageal cancer cell growth and invasion by repressing metadherin expression. Oncol Lett 13: 4769-4775, 2017.
APA
Hu, C., Lv, L., Peng, J., Liu, D., Wang, X., Zhou, Y., & Huo, J. (2017). MicroRNA-375 suppresses esophageal cancer cell growth and invasion by repressing metadherin expression. Oncology Letters, 13, 4769-4775. https://doi.org/10.3892/ol.2017.6098
MLA
Hu, C., Lv, L., Peng, J., Liu, D., Wang, X., Zhou, Y., Huo, J."MicroRNA-375 suppresses esophageal cancer cell growth and invasion by repressing metadherin expression". Oncology Letters 13.6 (2017): 4769-4775.
Chicago
Hu, C., Lv, L., Peng, J., Liu, D., Wang, X., Zhou, Y., Huo, J."MicroRNA-375 suppresses esophageal cancer cell growth and invasion by repressing metadherin expression". Oncology Letters 13, no. 6 (2017): 4769-4775. https://doi.org/10.3892/ol.2017.6098
Copy and paste a formatted citation
x
Spandidos Publications style
Hu C, Lv L, Peng J, Liu D, Wang X, Zhou Y and Huo J: MicroRNA-375 suppresses esophageal cancer cell growth and invasion by repressing metadherin expression. Oncol Lett 13: 4769-4775, 2017.
APA
Hu, C., Lv, L., Peng, J., Liu, D., Wang, X., Zhou, Y., & Huo, J. (2017). MicroRNA-375 suppresses esophageal cancer cell growth and invasion by repressing metadherin expression. Oncology Letters, 13, 4769-4775. https://doi.org/10.3892/ol.2017.6098
MLA
Hu, C., Lv, L., Peng, J., Liu, D., Wang, X., Zhou, Y., Huo, J."MicroRNA-375 suppresses esophageal cancer cell growth and invasion by repressing metadherin expression". Oncology Letters 13.6 (2017): 4769-4775.
Chicago
Hu, C., Lv, L., Peng, J., Liu, D., Wang, X., Zhou, Y., Huo, J."MicroRNA-375 suppresses esophageal cancer cell growth and invasion by repressing metadherin expression". Oncology Letters 13, no. 6 (2017): 4769-4775. https://doi.org/10.3892/ol.2017.6098
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