BRCA1 expression serves a role in vincristine resistance in colon cancer cells

  • Authors:
    • Zhongjie Xu
    • Lirong Zhang
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  • Published online on: May 10, 2017     https://doi.org/10.3892/ol.2017.6149
  • Pages: 345-348
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Abstract

The present study aimed to investigate the association between breast cancer susceptibility gene 1 (BRCA1) expression and drug resistance in colon cancer, with the specific aim of elucidating the underlying molecular mechanisms of vincristine (VCR) resistance in tumor cells. The HCT‑8 human colon cancer cell line was used to establish the VCR‑resistant HCT‑8/V line by gradually increasing the concentration of VCR during cell culture. The relative mRNA and protein expression levels of BRCA1 in these colon cancer cell lines was assessed by reverse transcriptase‑quantitative polymerase chain reaction (RT‑qPCR) analysis and western blotting, respectively. Resistance to VCR was established in the HCT‑8/V colon cancer cells, and RT‑qPCR and western blot analysis revealed the expression of BRCA1 to be significantly higher in the VCR‑resistant cells compared with their drug‑sensitive counterparts (P<0.05). The decreased BRCA1 expression in these VCR‑resistant cells may be associated with the drug resistance frequently observed in colon cancer.
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July-2017
Volume 14 Issue 1

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Xu Z and Xu Z: BRCA1 expression serves a role in vincristine resistance in colon cancer cells. Oncol Lett 14: 345-348, 2017
APA
Xu, Z., & Xu, Z. (2017). BRCA1 expression serves a role in vincristine resistance in colon cancer cells. Oncology Letters, 14, 345-348. https://doi.org/10.3892/ol.2017.6149
MLA
Xu, Z., Zhang, L."BRCA1 expression serves a role in vincristine resistance in colon cancer cells". Oncology Letters 14.1 (2017): 345-348.
Chicago
Xu, Z., Zhang, L."BRCA1 expression serves a role in vincristine resistance in colon cancer cells". Oncology Letters 14, no. 1 (2017): 345-348. https://doi.org/10.3892/ol.2017.6149