Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Oncology Letters
Join Editorial Board Propose a Special Issue
Print ISSN: 1792-1074 Online ISSN: 1792-1082
Journal Cover
July-2017 Volume 14 Issue 1

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
July-2017 Volume 14 Issue 1

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

Soluble urokinase‑type plasminogen activator receptor and urokinase‑type plasminogen activator receptor contribute to chemoresistance in leukemia

  • Authors:
    • Hong Guo
    • Lan‑Xia Zhou
    • Haizhen Ma
    • Bei Liu
    • Juan Cheng
    • Yun‑Yun Ma
    • Li Zhao
  • View Affiliations / Copyright

    Affiliations: Critical Care Medicine Department, The First Affiliated Hospital, Lanzhou University, Lanzhou, Gansu 730000, P.R. China, Central Laboratory, The First Affiliated Hospital, Lanzhou University, Lanzhou, Gansu 730000, P.R. China, Department of Hematology, The First Affiliated Hospital, Lanzhou University, Lanzhou, Gansu 730000, P.R. China
  • Pages: 383-389
    |
    Published online on: May 10, 2017
       https://doi.org/10.3892/ol.2017.6150
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

The soluble urokinase-type plasminogen activator receptor (suPAR) and the urokinase‑type plasminogen activator receptor (uPAR) have been proposed as useful biomarkers of tumor progression. Recently, suPAR was associated with chemoresistance in lung cancer. However, its clinical significance in leukemia has not previously been investigated. The present study examined the plasma levels of suPAR and the expression of the uPAR on bone marrow (BM) cells in 86 patients with leukemia at diagnosis prior to chemotherapy and 26 normal subjects (control group). The plasma suPAR levels were measured using ELISA, whilst uPAR expression was assayed by flow cytometry analysis. In addition, cell surface uPAR expression on K562 and multidrug‑resistant K562/ADM cell lines was studied by western blotting. On admission and follow-up, the levels of suPAR in patients with leukemia were significantly increased compared with controls. Systemic levels of suPAR were strongly associated with the numbers of white blood cells. A case was defined as uPAR‑positive (uPAR+) if >20% of the gated cells expressed uPAR. In comparison with 26 healthy BM samples that were negative for uPAR expression, 48 (55.8%) of the 86 leukemia patients were uPAR+. uPAR expression on the cell surface of multidrug‑resistant K562/ADM cells was increased compared with that on K562 cells. In conclusion, plasma suPAR expression may be a useful marker for subtype classification of patients with leukemia and cell surface uPAR may be associated with resistance to chemotherapy or disease progression.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

Figure 6

View References

1 

Montuori N and Ragno P: Multiple activities of a multifaceted receptor: Roles of cleaved and soluble uPAR. Front Biosci (Landmark Ed). 14:2494–2503. 2009. View Article : Google Scholar : PubMed/NCBI

2 

Rønne E, Pappot H, Grøndahl-Hansen J, Høyer-Hansen G, Plesner T, Hansen NE and Danø K: The receptor for urokinase plasminogen activator is present in plasma from healthy donors and elevated in patients with paroxymal nocturnal haemoglobinuria. Br J Haematol. 89:576–581. 1995. View Article : Google Scholar : PubMed/NCBI

3 

Stephens RW, Pedersen AN, Nielsen HJ, Hamers MJ, Høyer-Hansen G, Rønne E, Dybkjaer E, Danø K and Brünner N: ELISA determination of soluble urokinase receptor in blood from healthy donors and cancer patients. Clin Chem. 43:1868–1876. 1997.PubMed/NCBI

4 

Donadello K, Scoletta S, Covajes C and Vincent JL: suPAR as a prognostic marker in sepsis. BMC Med. 10:22012. View Article : Google Scholar : PubMed/NCBI

5 

Tzanakaki G, Paparoupa M, Kyprianou M, Barbouni A, Eugen-Olsen J and Kourea-Kremastinou J: Elevated soluble urokinase receptor values in CSF, age and bacterial meningitis infection are independent and additive risk factors of fatal outcome. Eur J Clin Microbiol Infect Dis. 31:1157–1162. 2012. View Article : Google Scholar : PubMed/NCBI

6 

Yılmaz G, Köksal I, Karahan SC and Mentese A: The diagnostic and prognostic significance of soluble urokinase plasminogen activator receptor in systemic inflammatory response syndrome. Clin Biochem. 44:1227–1230. 2011. View Article : Google Scholar : PubMed/NCBI

7 

Enocsson H, Sjöwall C and Wetterö J: Soluble urokinase plasminogen activator receptor-a valuable biomarker in systemic lupus erythematosus? Clin Chim Acta. 444:234–241. 2015. View Article : Google Scholar : PubMed/NCBI

8 

Toldi G, Bekő G, Kádár G, Mácsai E, Kovács L, Vásárhelyi B and Balog A: Soluble urokinase plasminogen activator receptor (suPAR) in the assessment of inflammatory activity of rheumatoid arthritis patients in remission. Clin Chem Lab Med. 51:327–332. 2013. View Article : Google Scholar : PubMed/NCBI

9 

Eugen-Olsen J, Andersen O, Linneberg A, Ladelund S, Hansen TW, Langkilde A, Petersen J, Pielak T, Møller LN, Jeppesen J, et al: Circulating soluble urokinase plasminogen activator receptor predicts cancer, cardiovascular disease, diabetes and mortality in the general population. J Intern Med. 268:296–308. 2010. View Article : Google Scholar : PubMed/NCBI

10 

Toldi G, Bíró E, Szalay B, Stenczer B, Molvarec A, Rigó J, Vásárhelyi B and Bekõ G: Soluble urokinase plasminogen activator receptor (suPAR) levels in healthy pregnancy and preeclampsia. Clin Chem Lab Med. 49:1873–1876. 2011. View Article : Google Scholar : PubMed/NCBI

11 

Ivancsó I, Toldi G, Bohács A, Eszes N, Müller V, Rigó J Jr, Vásárhelyi B, Losonczy G and Tamási L: Relationship of circulating soluble urokinase plasminogen activator receptor (suPAR) levels to disease control in asthma and asthmatic pregnancy. PLoS One. 8:e606972013. View Article : Google Scholar : PubMed/NCBI

12 

Odden N, Henriksen T and Mørkrid L: Serum soluble urokinase plasminogen activator receptor (suPAR) in early pregnancy prior to clinical onset of preeclampsia. Acta Obstet Gynecol Scand. 91:1226–1232. 2012. View Article : Google Scholar : PubMed/NCBI

13 

Pappot H, Høyer-Hansen G, Rønne E, Hansen HH, Brünner N, Danø K and Grøndahl-Hansen J: Elevated plasma levels of urokinase plasminogen activator receptor in non-small cell lung cancer patients. Eur J Cancer. 33:867–872. 1997. View Article : Google Scholar : PubMed/NCBI

14 

Høgdall CK, Christensen IJ, Stephens RW, Sørensen S, Nørgaard-Pedersen B and Nielsen HJ: Serum tetranectin is an independent prognostic marker in colorectal cancer and weakly correlated with plasma suPAR, plasma PAI-1 and serum CEA. APMIS. 110:630–638. 2002. View Article : Google Scholar : PubMed/NCBI

15 

Begum FD, Høgdall EV, Riisbro R, Christensen IJ, Engelholm SA, Jørgensen M, Pedersen BN and Høgdall CK: Prognostic value of plasma soluble urokinase plasminogen activator receptor (suPAR) in Danish patients with recurrent epithelial ovarian cancer (REOC). APMIS. 114:675–681. 2006. View Article : Google Scholar : PubMed/NCBI

16 

Ostrowski SR, Piironen T, Høyer-Hansen G, Gerstoft J, Pedersen BK and Ullum H: High plasma levels of intact and cleaved soluble urokinase receptor reflect immune activation and are independent predictors of mortality in HIV-1-infected patients. J Acquir Immune Defic Syndr. 39:23–31. 2005. View Article : Google Scholar : PubMed/NCBI

17 

Wittenhagen P, Kronborg G, Weis N, Nielsen H, Obel N, Pedersen SS and Eugen-Olsen J: The plasma level of soluble urokinase receptor is elevated in patients with Streptococcus pneumoniae bacteraemia and predicts mortality. Clin Microbiol Infect. 10:409–415. 2004. View Article : Google Scholar : PubMed/NCBI

18 

Koch A, Voigt S, Kruschinski C, Sanson E, Dückers H, Horn A, Yagmur E, Zimmermann H, Trautwein C and Tacke F: Circulating soluble urokinase plasminogen activator receptor is stably elevated during the first week of treatment in the intensive care unit and predicts mortality in critically ill patients. Crit Care. 15:R632011. View Article : Google Scholar : PubMed/NCBI

19 

Rabna P, Andersen A, Wejse C, Oliveira I, Gomes VF, Haaland MB, Aaby P and Eugen-Olsen J: High mortality risk among individuals assumed to be TB-negative can be predicted using a simple test. Trop Med Int Health. 14:986–994. 2009. View Article : Google Scholar : PubMed/NCBI

20 

Haupt TH, Petersen J, Ellekilde G, Klausen HH, Thorball CW, Eugen-Olsen J and Andersen O: Plasma suPAR levels are associated with mortality, admission time, and Charlson Comorbidity Index in the acutely admitted medical patient: A prospective observational study. Crit Care. 16:R1302012. View Article : Google Scholar : PubMed/NCBI

21 

Mustjoki S, Alitalo R, Stephens RW and Vaheri A: Blast cell-surface and plasma soluble urokinase receptor in acute leukemia patients: Relationship to classification and response to therapy. Thromb Haemost. 81:705–710. 1999.PubMed/NCBI

22 

Graf M, Reif S, Hecht K, Pelka-Fleischer R, Pfister K and Schmetzer H: High expression of urokinase plasminogen activator receptor (UPA-R) in acute myeloid leukemia (AML) is associated with worse prognosis. Am J Hematol. 79:26–35. 2005. View Article : Google Scholar : PubMed/NCBI

23 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(−Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

24 

Gutova M, Najbauer J, Gevorgyan A, Metz MZ, Weng Y, Shih CC and Aboody KS: Identification of uPAR-positive chemoresistant cells in small cell lung cancer. PLoS ONE. 2:e2432007. View Article : Google Scholar : PubMed/NCBI

25 

Lomholt Af, Christensen IJ, Hoyer-Hansen G and Nielsen HJ: Prognostic value of intact and leaved formof the urokinase plasminogen activator receptor in a rettospective study of 518 coloreta cancer patients. Act Oncol. 49:805–811. 2010. View Article : Google Scholar

26 

Kjellaman A, Akre O, Gustafsson O, Hansen GH, Lilja H, Norming U, Piironen T and Törnblom M: Soluble urokinase plasminogen activator receptor as prognositic marker in men participating in prostate cancer rescreening. J Intern med. 269:299–305. 2011. View Article : Google Scholar : PubMed/NCBI

27 

Kaya S, Köksal I, Menteşe A, Sönmez M, Sümer A, Yıldırım SS and Yılmaz G: The significance of serum urokinase plasminogen activation receptor (suPAR) in the diagnosis and follow-up of febrile neutropenic patients with hematologic malignancies. Int J Infect Dis. 17:e1056–e1059. 2013. View Article : Google Scholar : PubMed/NCBI

28 

Jardí M, Inglés-Esteve J, Burgal M, Azqueta C, Velasco F, López-Pedrera C, Miles LA and Félez J: Distinct patterns of urokinase receptor (uPAR) expression by leukemic cells and peripheral blood cells. Romb Haemost. 76:1009–1019. 1996.

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Guo H, Zhou LX, Ma H, Liu B, Cheng J, Ma YY and Zhao L: Soluble urokinase‑type plasminogen activator receptor and urokinase‑type plasminogen activator receptor contribute to chemoresistance in leukemia. Oncol Lett 14: 383-389, 2017.
APA
Guo, H., Zhou, L., Ma, H., Liu, B., Cheng, J., Ma, Y., & Zhao, L. (2017). Soluble urokinase‑type plasminogen activator receptor and urokinase‑type plasminogen activator receptor contribute to chemoresistance in leukemia. Oncology Letters, 14, 383-389. https://doi.org/10.3892/ol.2017.6150
MLA
Guo, H., Zhou, L., Ma, H., Liu, B., Cheng, J., Ma, Y., Zhao, L."Soluble urokinase‑type plasminogen activator receptor and urokinase‑type plasminogen activator receptor contribute to chemoresistance in leukemia". Oncology Letters 14.1 (2017): 383-389.
Chicago
Guo, H., Zhou, L., Ma, H., Liu, B., Cheng, J., Ma, Y., Zhao, L."Soluble urokinase‑type plasminogen activator receptor and urokinase‑type plasminogen activator receptor contribute to chemoresistance in leukemia". Oncology Letters 14, no. 1 (2017): 383-389. https://doi.org/10.3892/ol.2017.6150
Copy and paste a formatted citation
x
Spandidos Publications style
Guo H, Zhou LX, Ma H, Liu B, Cheng J, Ma YY and Zhao L: Soluble urokinase‑type plasminogen activator receptor and urokinase‑type plasminogen activator receptor contribute to chemoresistance in leukemia. Oncol Lett 14: 383-389, 2017.
APA
Guo, H., Zhou, L., Ma, H., Liu, B., Cheng, J., Ma, Y., & Zhao, L. (2017). Soluble urokinase‑type plasminogen activator receptor and urokinase‑type plasminogen activator receptor contribute to chemoresistance in leukemia. Oncology Letters, 14, 383-389. https://doi.org/10.3892/ol.2017.6150
MLA
Guo, H., Zhou, L., Ma, H., Liu, B., Cheng, J., Ma, Y., Zhao, L."Soluble urokinase‑type plasminogen activator receptor and urokinase‑type plasminogen activator receptor contribute to chemoresistance in leukemia". Oncology Letters 14.1 (2017): 383-389.
Chicago
Guo, H., Zhou, L., Ma, H., Liu, B., Cheng, J., Ma, Y., Zhao, L."Soluble urokinase‑type plasminogen activator receptor and urokinase‑type plasminogen activator receptor contribute to chemoresistance in leukemia". Oncology Letters 14, no. 1 (2017): 383-389. https://doi.org/10.3892/ol.2017.6150
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team