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Article

Upregulated expression of MMP-9 in gingival epithelial cells induced by prolonged stimulation with arecoline

  • Authors:
    • Osamu Uehara
    • Kousuke Takimoto
    • Tetsuro Morikawa
    • Fumiya Harada
    • Rie Takai
    • Bhoj Raj Adhikari
    • Ryoko Itatsu
    • Tomohisa Nakamura
    • Koki Yoshida
    • Hirofumi Matsuoka
    • Hiroki Nagayasu
    • Ichiro Saito
    • Malsantha Muthumala
    • Itsuo Chiba
    • Yoshihiro Abiko
  • View Affiliations / Copyright

    Affiliations: Division of Disease Control and Molecular Epidemiology, Department of Oral Growth and Development, School of Dentistry, Health Sciences University of Hokkaido, Tobetsu, Hokkaido 061‑0293, Japan, Division of Oral and Maxillofacial Surgery, Department of Human Biology and Pathophysiology, School of Dentistry, Health Sciences University of Hokkaido, Tobetsu, Hokkaido 061‑0293, Japan, Division of Oral Medicine and Pathology, Department of Human Biology and Pathophysiology, School of Dentistry, Health Sciences University of Hokkaido, Tobetsu, Hokkaido 061‑0293, Japan, Department of Pathology, Tsurumi University School of Dental Medicine, Yokohama, Kanagawa 230‑8501, Japan, Department of Oral and Maxillofacial Surgery, Army Hospital, Colombo, Sri Lanka
  • Pages: 1186-1192
    |
    Published online on: May 17, 2017
       https://doi.org/10.3892/ol.2017.6194
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Abstract

Betel quid chewing is implicated in the high prevalence of oral cancer in Southeast Asian countries. One of the major components of betel quid is arecoline. In the present study, in order to characterize the association between chronic arecoline stimulation and carcinogenesis the expression level of matrix metalloproteinase (MMP)‑2, MMP‑9, tissue inhibitor of metalloproteinase (TIMP)‑1 and TIMP‑2 mRNA in human gingival epithelial progenitor cells (HGEPs) stimulated with arecoline was assessed. The HGEPs were alternated between 3 days of incubation with arecoline (50 µg/ml), and 3 days without arecoline, for up to 30 days. The expression levels of the MMPs and TIMPs in the cells stimulated with arecoline were evaluated by reverse transcription-quantitative polymerase chain reaction at 18 and 30 days. The expression of MMP‑9 mRNA in the experimental group was significantly increased compared with in the control group (P<0.01). No significant differences in the expression of MMP‑2, TIMP‑1 or TIMP‑2 mRNA were observed between the experimental and control groups. Using an MMP‑9 activity assay, the levels of MMP‑9 activity in the experimental group were demonstrated to be significantly higher than in the control group (P<0.05). To investigate associated cellular signaling pathways, PDTC [a nuclear factor (NF)‑κB/inhibitor of NF‑κB (IκB) inhibitor], PD98059 [a mitogen-activated protein kinase kinase (MAPKK)1 and MAPKK2 inhibitor], SB203580 (a p38 MAPK inhibitor) and 5,15‑DPP [a signal transduction and activator of transcription (STAT) 3 inhibitor] were used. All inhibitors decreased the extent of MMP‑9 upregulation induced by stimulation with arecoline. Based on the data, it is hypothesized that MMP‑9 activity may be involved in the pathological alterations of oral epithelium induced by betel quid chewing, and that the NF‑κB/IκB, MAPK, p38 MAPK and STAT3 signaling pathways may be involved in the production of MMP-9 induced by betel quid chewing.
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1 

Chiba I, Muthumala M, Yamazaki Y, Uz Zaman A, Iizuka T, Amemiya A, Shibata T, Kashiwazaki H, Sugiura C and Fukuda H: Characteristics of mutations in the p53 gene of oral squamous-cell carcinomas associated with betel-quid chewing in Sri Lanka. Int J Cancer. 77:839–842. 1998. View Article : Google Scholar : PubMed/NCBI

2 

IARC Monographs on the Evaluation of the Carcinogenic Risk of Chemicals to Human: Tobacco Habits Other than Smoking; Betel-Quid and Areca-Nut Chewing; and Some Related Nitrosamines. 37. IARC; Lyon: pp. 141–200. 1985

3 

Ariyawardana A, Athukorala AD and Arulanandam A: Effect of betel chewing, tobacco smoking and alcohol consumption on oral submucous fibrosis: A case-control study in Sri Lanka. J Oral Pathol Med. 35:197–201. 2006. View Article : Google Scholar : PubMed/NCBI

4 

Chung CH, Yang YH, Wang TY, Shieh TY and Warnakulasuriya S: Oral precancerous disorders associated with areca quid chewing, smoking, and alcohol drinking in southern Taiwan. J Oral Pathol Med. 34:460–466. 2005. View Article : Google Scholar : PubMed/NCBI

5 

Gupta B and Johnson NW: Systematic review and meta-analysis of association of smokeless tobacco and of betel quid without tobacco with incidence of oral cancer in South Asia and the Pacific. PLoS One. 9:e1133852014. View Article : Google Scholar : PubMed/NCBI

6 

Wollina U, Verma SB, Ali FM and Patil K: Oral submucous fibrosis: An update. Clin Cosmet Investig Dermatol. 8:193–204. 2015. View Article : Google Scholar : PubMed/NCBI

7 

Chang MC, Chan CP, Wang WT, Chang BE, Lee JJ, Tseng SK, Yeung SY, Hahn LJ and Jeng JH: Toxicity of areca nut ingredients: Activation of CHK1/CHK2, induction of cell cycle arrest, and regulation of MMP-9 and TIMPs production in SAS epithelial cells. Head Neck. 35:1295–1302. 2013. View Article : Google Scholar : PubMed/NCBI

8 

Thangjam GS and Kondaiah P: Regulation of oxidative-stress responsive genes by arecoline in human keratinocytes. J Periodontal Res. 44:673–682. 2009. View Article : Google Scholar : PubMed/NCBI

9 

Topcu Z, Chiba I, Fujieda M, Shibata T, Ariyoshi N, Yamazaki H, Sevgican F, Muthumala M, Kobayashi H and Kamataki T: CYP2A6 gene deletion reduces oral cancer risk in betel quid chewers in Sri Lanka. Carcinogenesis. 23:595–598. 2002. View Article : Google Scholar : PubMed/NCBI

10 

Takeshima M, Saitoh M, Kusano K, Nagayasu H, Kurashige Y, Malsantha M, Arakawa T, Takuma T, Chiba I, Kaku T, et al: High frequency of hypermethylation of p14, p15 and p16 in oral pre-cancerous lesions associated with betel-quid chewing in Sri Lanka. J Oral Pathol Med. 37:475–479. 2008. View Article : Google Scholar : PubMed/NCBI

11 

Shieh DH, Chiang LC and Shieh TY: Augmented mRNA expression of tissue inhibitor of metalloproteinase-1 in buccal mucosal fibroblasts by arecoline and safrole as a possible pathogenesis for oral submucous fibrosis. Oral Oncol. 39:728–735. 2003. View Article : Google Scholar : PubMed/NCBI

12 

Groblewska M, Siewko M, Mroczko B and Szmitkowski M: The role of matrix metalloproteinases (MMPs) and their inhibitors (TIMPs) in the development of esophageal cancer. Folia Histochem Cytobiol. 50:12–19. 2012. View Article : Google Scholar : PubMed/NCBI

13 

Herszényi L, Hritz I, Lakatos G, Varga MZ and Tulassay Z: The behavior of matrix metalloproteinases and their inhibitors in colorectal cancer. Int J Mol Sci. 13:13240–13263. 2012. View Article : Google Scholar : PubMed/NCBI

14 

Lee SS, Tsai CH, Yu CC, Ho YC, Hsu HI and Chang YC: The expression of O(6)-methylguanine-DNA methyltransferase in human oral keratinocytes stimulated with arecoline. J Oral Pathol Med. 42:600–605. 2013. View Article : Google Scholar : PubMed/NCBI

15 

Chen PH, Lee KW, Hsu CC, Chen JY, Wang YH, Chen KK, Wang HM, Huang HW and Huang B: Expression of a splice variant of CYP26B1 in betel quid-related oral cancer. ScientificWorldJournal. 2014:8105612014.PubMed/NCBI

16 

Chen PH, Huang B, Shieh TY, Wang YH, Chen YK, Wu JH, Huang JH, Chen CC and Lee KW: The influence of monoamine oxidase variants on the risk of betel quid-associated oral and pharyngeal cancer. ScientificWorldJournal. 2014:1835482014. View Article : Google Scholar : PubMed/NCBI

17 

Takai R, Uehara O, Harada F, Utsunomiya M, Chujo T, Yoshida K, Sato J, Nishimura M, Chiba I and Abiko Y: DNA hypermethylation of extracellular matrix-related genes in human periodontal fibroblasts induced by stimulation for a prolonged period with lipopolysaccharide derived from Porphyromonas gingivalis. J Periodontal Res. 51:508–517. 2016. View Article : Google Scholar : PubMed/NCBI

18 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(−Delta Delta C(T)) Method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

19 

Rehman A, Ali S, Lone MA, Atif M, Hassona Y, Prime SS, Pitiyage GN, James EL and Parkinson EK: Areca nut alkaloids induce irreparable DNA damage and senescence in fibroblasts and may create a favourable environment for tumour progression. J Oral Pathol Med. 45:365–372. 2016. View Article : Google Scholar : PubMed/NCBI

20 

Tseng YH, Chang KW, Liu CJ, Lin CY, Yang SC and Lin SC: Areca nut extract represses migration and differentiation while activating matrix metalloproteinase-9 of normal gingival epithelial cells. J Periodontal Res. 43:490–499. 2008.PubMed/NCBI

21 

Lukaszewicz-Zając M, Mroczko B and Szmitkowski M: Gastric cancer - The role of matrix metalloproteinases in tumor progression. Clin Chim Acta. 412:1725–1730. 2011. View Article : Google Scholar : PubMed/NCBI

22 

Li Y, Ma J, Guo Q, Duan F, Tang F, Zheng P, Zhao Z and Lu G: Overexpression of MMP-2 and MMP-9 in esophageal squamous cell carcinoma. Dis Esophagus. 22:664–667. 2009. View Article : Google Scholar : PubMed/NCBI

23 

Chen Y, Zhang W, Geng N, Tian K and Windsor L Jack: MMPs, TIMP-2, and TGF-beta1 in the cancerization of oral lichen planus. Head Neck. 30:1237–1245. 2008. View Article : Google Scholar : PubMed/NCBI

24 

Fracalossi AC, Miranda SR, Oshima CT, Franco M and Ribeiro DA: The role of matrix metalloproteinases 2 and 9 during rat tongue carcinogenesis induced by 4-nitroquinoline 1-oxide. J Mol Histol. 41:19–25. 2010. View Article : Google Scholar : PubMed/NCBI

25 

Jordan RC, Macabeo-Ong M, Shiboski CH, Dekker N, Ginzinger DG, Wong DT and Schmidt BL: Overexpression of matrix metalloproteinase-1 and -9 mRNA is associated with progression of oral dysplasia to cancer. Clin Cancer Res. 10:6460–6465. 2004. View Article : Google Scholar : PubMed/NCBI

26 

Hohberger L, Wuertz BR, Xie H, Griffin T and Ondrey F: TNF-alpha drives matrix metalloproteinase-9 in squamous oral carcinogenesis. Laryngoscope. 118:1395–1399. 2008. View Article : Google Scholar : PubMed/NCBI

27 

Liu SY, Lin MH, Yang SC, Huang GC, Chang L, Chang S, Yen CY, Chiang WF, Kuo YY, Chen LL, et al: Increased expression of matrix metalloproteinase-2 in oral cells after short-term stimulation and long-term usage of areca quid. J Formos Med Assoc. 104:390–397. 2005.PubMed/NCBI

28 

Sawicki G, Marcoux Y, Sarkhosh K, Tredget EE and Ghahary A: Interaction of keratinocytes and fibroblasts modulates the expression of matrix metalloproteinases-2 and -9 and their inhibitors. Mol Cell Biochem. 269:209–216. 2005. View Article : Google Scholar : PubMed/NCBI

29 

Lai WW, Hsu SC, Chueh FS, Chen YY, Yang JS, Lin JP, Lien JC, Tsai CH and Chung JG: Quercetin inhibits migration and invasion of SAS human oral cancer cells through inhibition of NF-κB and matrix metalloproteinase-2/−9 signaling pathways. Anticancer Res. 33:1941–1950. 2013.PubMed/NCBI

30 

Lu LC, Yang CW, Hsieh WY, Chuang WH, Lin YC and Lin CS: Decreases in plasma MMP-2/TIMP-2 and MMP-9/TIMP-1 ratios in uremic patients during hemodialysis. Clin Exp Nephrol. 20:934–942. 2016. View Article : Google Scholar : PubMed/NCBI

31 

Li S, Li C, Ryu HH, Lim SH, Jang WY and Jung S: Bacitracin inhibits the migration of U87-MG Glioma cells via interferences of the integrin outside-in signaling pathway. J Korean Neurosurg Soc. 59:106–116. 2016. View Article : Google Scholar : PubMed/NCBI

32 

Mukhopadhyay P, Rajesh M, Bátkai S, Kashiwaya Y, Haskó G, Liaudet L, Szabó C and Pacher P: Role of superoxide, nitric oxide, and peroxynitrite in doxorubicin-induced cell death in vivo and in vitro. Am J Physiol Heart Circ Physiol. 296:H1466–H1483. 2009. View Article : Google Scholar : PubMed/NCBI

33 

Chen KC, Wang YS, Hu CY, Chang WC, Liao YC, Dai CY and Juo SH: OxLDL up-regulates microRNA-29b, leading to epigenetic modifications of MMP-2/MMP-9 genes: A novel mechanism for cardiovascular diseases. FASEB J. 25:1718–1728. 2011. View Article : Google Scholar : PubMed/NCBI

34 

Scrideli CA, Cortez MA, Yunes JA, Queiróz RG, Valera ET, da Mata JF, Toledo SR, Pavoni-Ferreira P, Lee ML, Petrilli AS, et al: mRNA expression of matrix metalloproteinases (MMPs) 2 and 9 and tissue inhibitor of matrix metalloproteinases (TIMPs) 1 and 2 in childhood acute lymphoblastic leukemia: Potential role of TIMP1 as an adverse prognostic factor. Leuk Res. 34:32–37. 2010. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Uehara O, Takimoto K, Morikawa T, Harada F, Takai R, Adhikari BR, Itatsu R, Nakamura T, Yoshida K, Matsuoka H, Matsuoka H, et al: Upregulated expression of MMP-9 in gingival epithelial cells induced by prolonged stimulation with arecoline. Oncol Lett 14: 1186-1192, 2017.
APA
Uehara, O., Takimoto, K., Morikawa, T., Harada, F., Takai, R., Adhikari, B.R. ... Abiko, Y. (2017). Upregulated expression of MMP-9 in gingival epithelial cells induced by prolonged stimulation with arecoline. Oncology Letters, 14, 1186-1192. https://doi.org/10.3892/ol.2017.6194
MLA
Uehara, O., Takimoto, K., Morikawa, T., Harada, F., Takai, R., Adhikari, B. R., Itatsu, R., Nakamura, T., Yoshida, K., Matsuoka, H., Nagayasu, H., Saito, I., Muthumala, M., Chiba, I., Abiko, Y."Upregulated expression of MMP-9 in gingival epithelial cells induced by prolonged stimulation with arecoline". Oncology Letters 14.1 (2017): 1186-1192.
Chicago
Uehara, O., Takimoto, K., Morikawa, T., Harada, F., Takai, R., Adhikari, B. R., Itatsu, R., Nakamura, T., Yoshida, K., Matsuoka, H., Nagayasu, H., Saito, I., Muthumala, M., Chiba, I., Abiko, Y."Upregulated expression of MMP-9 in gingival epithelial cells induced by prolonged stimulation with arecoline". Oncology Letters 14, no. 1 (2017): 1186-1192. https://doi.org/10.3892/ol.2017.6194
Copy and paste a formatted citation
x
Spandidos Publications style
Uehara O, Takimoto K, Morikawa T, Harada F, Takai R, Adhikari BR, Itatsu R, Nakamura T, Yoshida K, Matsuoka H, Matsuoka H, et al: Upregulated expression of MMP-9 in gingival epithelial cells induced by prolonged stimulation with arecoline. Oncol Lett 14: 1186-1192, 2017.
APA
Uehara, O., Takimoto, K., Morikawa, T., Harada, F., Takai, R., Adhikari, B.R. ... Abiko, Y. (2017). Upregulated expression of MMP-9 in gingival epithelial cells induced by prolonged stimulation with arecoline. Oncology Letters, 14, 1186-1192. https://doi.org/10.3892/ol.2017.6194
MLA
Uehara, O., Takimoto, K., Morikawa, T., Harada, F., Takai, R., Adhikari, B. R., Itatsu, R., Nakamura, T., Yoshida, K., Matsuoka, H., Nagayasu, H., Saito, I., Muthumala, M., Chiba, I., Abiko, Y."Upregulated expression of MMP-9 in gingival epithelial cells induced by prolonged stimulation with arecoline". Oncology Letters 14.1 (2017): 1186-1192.
Chicago
Uehara, O., Takimoto, K., Morikawa, T., Harada, F., Takai, R., Adhikari, B. R., Itatsu, R., Nakamura, T., Yoshida, K., Matsuoka, H., Nagayasu, H., Saito, I., Muthumala, M., Chiba, I., Abiko, Y."Upregulated expression of MMP-9 in gingival epithelial cells induced by prolonged stimulation with arecoline". Oncology Letters 14, no. 1 (2017): 1186-1192. https://doi.org/10.3892/ol.2017.6194
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