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Remodeling of energy metabolism by a ketone body and medium-chain fatty acid suppressed the proliferation of CT26 mouse colon cancer cells

  • Authors:
    • Yui Kadochi
    • Shiori Mori
    • Rina Fujiwara‑Tani
    • Yi Luo
    • Yukiko Nishiguchi
    • Shingo Kishi
    • Kiyomu Fujii
    • Hitoshi Ohmori
    • Hiroki Kuniyasu
  • View Affiliations / Copyright

    Affiliations: Department of Molecular Pathology, Nara Medical University, Kashihara, Nara 634‑8521, Japan
    Copyright: © Kadochi et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 673-680
    |
    Published online on: May 17, 2017
       https://doi.org/10.3892/ol.2017.6195
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Abstract

Normal and cancerous cells are suggested to have differential utilization of fatty acids and ketone bodies, which could be exploited in cancer therapy. The present study examined the effect of 3‑hydroxybutyric acid (3‑HBA), which is a ketone body generating acetyl‑CoA, and lauric acid (LAA, C12:0), which is a medium‑chain saturated fatty acid translocated to mitochondria in a carnitine‑independent manner to produce acetyl‑CoA, on the energy metabolism of mouse CT26 colon cancer cells. In CT26 cells expressing 3‑HBA and LAA transporters, 3‑HBA and LAA reduced cell proliferation, mitochondrial volume and lactate production, and increased oxidative stress, particularly in low‑glucose conditions. Concurrent treatment with 3‑HBA and LAA under glucose starvation had a synergistic effect on cell growth inhibition. In addition, LAA and LAA + 3‑HBA promoted an imbalance in the expression of enzymes in the electron transport chain. These findings suggested that treatment with 3‑HBA and/or LAA during glucose starvation may reprogram energy metabolism and decrease the proliferation of cancer cells.
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1 

Chen X, Qian Y and Wu S: The Warburg effect: Evolving interpretations of an established concept. Free Radic Biol Med. 79:253–263. 2015. View Article : Google Scholar : PubMed/NCBI

2 

Warburg O: On respiratory impairment in cancer cells. Science. 124:269–270. 1956.PubMed/NCBI

3 

Hess JA and Khasawneh MK: Cancer metabolism and oxidative stress: Insights into carcinogenesis and chemotherapy via the non-dihydrofolate reductase effects of methotrexate. BBA Clin. 3:152–161. 2015. View Article : Google Scholar : PubMed/NCBI

4 

Boland ML, Chourasia AH and Macleod KF: Mitochondrial dysfunction in cancer. Front Oncol. 3:2922013. View Article : Google Scholar : PubMed/NCBI

5 

Dueregger A, Schöpf B, Eder T, Höfer J, Gnaiger E, Aufinger A, Kenner L, Perktold B, Ramoner R, Klocker H and Eder IE: Differential utilization of dietary fatty acids in benign and malignant cells of the prostate. PLoS One. 10:e01357042015. View Article : Google Scholar : PubMed/NCBI

6 

Papamandjaris AA, MacDougall DE and Jones PJ: Medium chain fatty acid metabolism and energy expenditure: Obesity treatment implications. Life Sci. 62:1203–1215. 1998. View Article : Google Scholar : PubMed/NCBI

7 

Newman JC and Verdin E: β-hydroxybutyrate: Much more than a metabolite. Diabetes Res Clin Pract. 106:173–181. 2014. View Article : Google Scholar : PubMed/NCBI

8 

Fukao T, Mitchell G, Sass JO, Hori T, Orii K and Aoyama Y: Ketone body metabolism and its defects. J Inherit Metab Dis. 37:541–551. 2014. View Article : Google Scholar : PubMed/NCBI

9 

McCarty MF and DiNicolantonio JJ: Lauric acid-rich medium-chain triglycerides can substitute for other oils in cooking applications and may have limited pathogenicity. Open Heart. 3:e0004672016. View Article : Google Scholar : PubMed/NCBI

10 

Fulgencio JP, Kohl C, Girard J and Pégorier JP: Troglitazone inhibits fatty acid oxidation and esterification, and gluconeogenesis in isolated hepatocytes from starved rats. Diabetes. 45:1556–1562. 1996. View Article : Google Scholar : PubMed/NCBI

11 

Kuniyasu H, Yano S, Sasaki T, Sasahira T, Sone S and Ohmori H: Colon cancer cell-derived high mobility group 1/amphoterin induces growth inhibition and apoptosis in macrophages. Am J Pathol. 166:751–760. 2005. View Article : Google Scholar : PubMed/NCBI

12 

Kuniyasu H, Oue N, Wakikawa A, Shigeishi H, Matsutani N, Kuraoka K, Ito R, Yokozaki H and Yasui W: Expression of receptors for advanced glycation end-products (RAGE) is closely associated with the invasive and metastatic activity of gastric cancer. J Pathol. 196:163–170. 2002. View Article : Google Scholar : PubMed/NCBI

13 

Schutkowski A, Wege N, Stangl GI and König B: Tissue-specific expression of monocarboxylate transporters during fasting in mice. PLoS One. 9:e1121182014. View Article : Google Scholar : PubMed/NCBI

14 

Takimoto M and Hamada T: Acute exercise increases brain region-specific expression of MCT1, MCT2, MCT4, GLUT1, and COX IV proteins. J Appl Physiol (1985). 116:1238–1250. 2014. View Article : Google Scholar : PubMed/NCBI

15 

Schönfeld P and Wojtczak L: Short- and medium-chain fatty acids in energy metabolism: The cellular perspective. J Lipid Res. 57:943–954. 2016. View Article : Google Scholar : PubMed/NCBI

16 

Sawai M, Yashiro M, Nishiguchi Y, Ohira M and Hirakawa K: Growth-inhibitory effects of the ketone body, monoacetoacetin, on human gastric cancer cells with succinyl-CoA: 3-oxoacid CoA-transferase (SCOT) deficiency. Anticancer Res. 24:2213–2217. 2004.PubMed/NCBI

17 

Ṧtefková K, Procházková J and Pacherník J: Alkaline Phosphatase in Stem Cells. Stem Cell Int. 2015:6283682015.

18 

Skinner R, Trujillo A, Ma X and Beierle EA: Ketone bodies inhibit the viability of human neuroblastoma cells. J Pediatr Surg. 44:212–216. 2009. View Article : Google Scholar : PubMed/NCBI

19 

Chao C, Carmical JR, Ives KL, Wood TG, Aronson JF, Gomez GA, Djukom CD and Hellmich MR: CD133+ colon cancer cells are more interactive with the tumor microenvironment than CD133- cells. Lab Invest. 92:420–436. 2012. View Article : Google Scholar : PubMed/NCBI

20 

Park JH, Vithayathil S, Kumar S, Sung PL, Dobrolecki LE, Putluri V, Bhat VB, Bhowmik SK, Gupta V, Arora K, et al: Fatty acid oxidation-driven src links mitochondrial energy reprogramming and oncogenic properties in triple-negative breast cancer. Cell Rep. 14:2154–2165. 2016. View Article : Google Scholar : PubMed/NCBI

21 

Carneiro L, Geller S, Fioramonti X, Hébert A, Repond C, Leloup C and Pellerin L: Evidence for hypothalamic ketone body sensing: Impact on food intake and peripheral metabolic responses in mice. Am J Physiol Endocrinol Metab. 310:E103–E115. 2016.PubMed/NCBI

22 

Allen BG, Bhatia SK, Anderson CM, Eichenberger-Gilmore JM, Sibenaller ZA, Mapuskar KA, Schoenfeld JD, Buatti JM, Spitz DR and Fath MA: Ketogenic diets as an adjuvant cancer therapy: History and potential mechanism. Redox Biol. 2:963–970. 2014. View Article : Google Scholar : PubMed/NCBI

23 

Hecker M, Sommer N, Voigtmann H, Pak O, Mohr A, Wolf M, Vadász I, Herold S, Weissmann N, Morty RE, et al: Impact of short- and medium-chain fatty acids on mitochondrial function in severe inflammation. JPEN J Parenter Enteral Nutr. 38:587–594. 2014. View Article : Google Scholar : PubMed/NCBI

24 

Ishizawa R, Masuda K, Sakata S and Nakatani A: Effects of different fatty acid chain lengths on fatty acid oxidation-related protein expression levels in rat skeletal muscles. J Oleo Sci. 64:415–421. 2015. View Article : Google Scholar : PubMed/NCBI

25 

Provenzani A, Fronza R, Loreni F, Pascale A, Amadio M and Quattrone A: Global alterations in mRNA polysomal recruitment in a cell model of colorectal cancer progression to metastasis. Carcinogenesis. 27:1323–1333. 2006. View Article : Google Scholar : PubMed/NCBI

26 

Chen Y, Zhang H, Zhou HJ, Ji W and Min W: Mitochondrial redox signaling and tumor progression. Cancers (Basel). 8:pii: E402016. View Article : Google Scholar

27 

Schonfeld P and Wojtczak L: Brown adipose tissue mitochondria oxidizing fatty acids generate high levels of reactive oxygen species irrespective of the uncoupling protein-1 activity state. Biochim Biophys Acta. 1817:410–418. 2012. View Article : Google Scholar : PubMed/NCBI

28 

Lee YK, Jee BA, Kwon SM, Yoon YS, Xu WG, Wang HJ, Wang XW, Thorgeirsson SS, Lee JS, Woo HG and Yoon G: Identification of a mitochondrial defect gene signature reveals NUPR1 as a key regulator of liver cancer progression. Hepatology. 62:1174–1189. 2015. View Article : Google Scholar : PubMed/NCBI

29 

Ploumi C, Daskalaki I and Tavernarakis N: Mitochondrial biogenesis and clearance: A balancing act. FEBS J. 284:183–195. 2017. View Article : Google Scholar : PubMed/NCBI

30 

Ghanta S, Tsoyi K, Liu X, Nakahira K, Ith B, Coronata AA, Fredenburgh LE, Englert JA, Piantadosi CA, Choi AM and Perrella MA: Mesenchymal stromal cells deficient in autophagy proteins are susceptible to oxidative injury and mitochondrial dysfunction. Am J Respir Cell Mol Biol. 56:300–309. 2016. View Article : Google Scholar

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Copy and paste a formatted citation
Spandidos Publications style
Kadochi Y, Mori S, Fujiwara‑Tani R, Luo Y, Nishiguchi Y, Kishi S, Fujii K, Ohmori H and Kuniyasu H: Remodeling of energy metabolism by a ketone body and medium-chain fatty acid suppressed the proliferation of CT26 mouse colon cancer cells. Oncol Lett 14: 673-680, 2017.
APA
Kadochi, Y., Mori, S., Fujiwara‑Tani, R., Luo, Y., Nishiguchi, Y., Kishi, S. ... Kuniyasu, H. (2017). Remodeling of energy metabolism by a ketone body and medium-chain fatty acid suppressed the proliferation of CT26 mouse colon cancer cells. Oncology Letters, 14, 673-680. https://doi.org/10.3892/ol.2017.6195
MLA
Kadochi, Y., Mori, S., Fujiwara‑Tani, R., Luo, Y., Nishiguchi, Y., Kishi, S., Fujii, K., Ohmori, H., Kuniyasu, H."Remodeling of energy metabolism by a ketone body and medium-chain fatty acid suppressed the proliferation of CT26 mouse colon cancer cells". Oncology Letters 14.1 (2017): 673-680.
Chicago
Kadochi, Y., Mori, S., Fujiwara‑Tani, R., Luo, Y., Nishiguchi, Y., Kishi, S., Fujii, K., Ohmori, H., Kuniyasu, H."Remodeling of energy metabolism by a ketone body and medium-chain fatty acid suppressed the proliferation of CT26 mouse colon cancer cells". Oncology Letters 14, no. 1 (2017): 673-680. https://doi.org/10.3892/ol.2017.6195
Copy and paste a formatted citation
x
Spandidos Publications style
Kadochi Y, Mori S, Fujiwara‑Tani R, Luo Y, Nishiguchi Y, Kishi S, Fujii K, Ohmori H and Kuniyasu H: Remodeling of energy metabolism by a ketone body and medium-chain fatty acid suppressed the proliferation of CT26 mouse colon cancer cells. Oncol Lett 14: 673-680, 2017.
APA
Kadochi, Y., Mori, S., Fujiwara‑Tani, R., Luo, Y., Nishiguchi, Y., Kishi, S. ... Kuniyasu, H. (2017). Remodeling of energy metabolism by a ketone body and medium-chain fatty acid suppressed the proliferation of CT26 mouse colon cancer cells. Oncology Letters, 14, 673-680. https://doi.org/10.3892/ol.2017.6195
MLA
Kadochi, Y., Mori, S., Fujiwara‑Tani, R., Luo, Y., Nishiguchi, Y., Kishi, S., Fujii, K., Ohmori, H., Kuniyasu, H."Remodeling of energy metabolism by a ketone body and medium-chain fatty acid suppressed the proliferation of CT26 mouse colon cancer cells". Oncology Letters 14.1 (2017): 673-680.
Chicago
Kadochi, Y., Mori, S., Fujiwara‑Tani, R., Luo, Y., Nishiguchi, Y., Kishi, S., Fujii, K., Ohmori, H., Kuniyasu, H."Remodeling of energy metabolism by a ketone body and medium-chain fatty acid suppressed the proliferation of CT26 mouse colon cancer cells". Oncology Letters 14, no. 1 (2017): 673-680. https://doi.org/10.3892/ol.2017.6195
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