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The novel immunomodulator IMMUNEPOTENT CRP combined with chemotherapy agent increased the rate of immunogenic cell death and prevented melanoma growth

  • Authors:
    • Maria del Carmen Rodríguez‑Salazar
    • Moises Armides Franco‑Molina
    • Edgar Mendoza‑Gamboa
    • Ana Carolina Martínez‑Torres
    • Pablo Zapata‑Benavides
    • Jose Sullivan López‑González
    • Erika Evangelina Coronado‑Cerda
    • Juan Manuel Alcocer‑González
    • Reyes Silvestre Tamez‑Guerra
    • Cristina Rodríguez‑Padilla
  • View Affiliations / Copyright

    Affiliations: Department of Immunology and Virology, Biological Sciences Faculty, Autonomous University of Nuevo Leon, San Nicolas de los Garza, Nuevo Leon 66455, Mexico, Lung Cancer Laboratory, National Institute of Respiratory Diseases ‘Ismael Cosio Villegas’, Mexico City 14080, Mexico
    Copyright: © Rodríguez‑Salazar et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 844-852
    |
    Published online on: May 18, 2017
       https://doi.org/10.3892/ol.2017.6202
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Abstract

Immunogenic cell death is a cell death modality that stimulates the immune system to combat cancer cells. IMMUNEPOTENT CRP (ICRP) is a mixture of substances of low molecular weight obtained from bovine spleens that exhibits in vitro cytotoxic activity on different tumor cell lines and modulates the immune response in vivo. The aim of the present study was to determine whether the cytotoxic effect of ICRP and its combination with oxaliplatin (OXP) on murine melanoma B16F10 cells was due to immunogenic cell death. The cytotoxic assay was performed using flow cytometry to detect Annexin V and propidium iodide staining, and calreticulin (CRT) exposure. Adenosine triphosphate, heat shock protein (HSP) 70, HSP90 and high mobility group box 1 (HMGB1) release were identified using bioluminescence, western blot and ELISA assays, respectively. The present in vitro study demonstrated that treatments with ICRP or OXP induced cell death in a time‑dependent manner, but treatment with the combination of ICRP + OXP increased the cytotoxic effect following 24 h of treatment. CRT exposure and release of adenosine triphosphate (ATP), HSP70, HSP90 and HMGB1 were induced by treatment with ICRP, and the combination of ICRP + OXP increased the exposure and release of damage-associated molecular patterns (DAMPs), while OXP treatment only induced CRT exposure, ATP and HMGB1 release. The in vivo experiments demonstrated that administration of tumor‑derived DAMP‑rich cell lysates derived from B16F10 cells treated with ICRP and the combination of ICRP + OXP prevented melanoma growth; however, OXP treatment did not. These results suggested that IMMUNEPOTENT CRP may be used as an agent to increase the ability of antitumor drugs to induce immunogenic cell death and prevent the growth of melanoma.
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Spandidos Publications style
Rodríguez‑Salazar Md, Franco‑Molina MA, Mendoza‑Gamboa E, Martínez‑Torres AC, Zapata‑Benavides P, López‑González JS, Coronado‑Cerda EE, Alcocer‑González JM, Tamez‑Guerra RS, Rodríguez‑Padilla C, Rodríguez‑Padilla C, et al: The novel immunomodulator IMMUNEPOTENT CRP combined with chemotherapy agent increased the rate of immunogenic cell death and prevented melanoma growth. Oncol Lett 14: 844-852, 2017.
APA
Rodríguez‑Salazar, M.d., Franco‑Molina, M.A., Mendoza‑Gamboa, E., Martínez‑Torres, A.C., Zapata‑Benavides, P., López‑González, J.S. ... Rodríguez‑Padilla, C. (2017). The novel immunomodulator IMMUNEPOTENT CRP combined with chemotherapy agent increased the rate of immunogenic cell death and prevented melanoma growth. Oncology Letters, 14, 844-852. https://doi.org/10.3892/ol.2017.6202
MLA
Rodríguez‑Salazar, M. d., Franco‑Molina, M. A., Mendoza‑Gamboa, E., Martínez‑Torres, A. C., Zapata‑Benavides, P., López‑González, J. S., Coronado‑Cerda, E. E., Alcocer‑González, J. M., Tamez‑Guerra, R. S., Rodríguez‑Padilla, C."The novel immunomodulator IMMUNEPOTENT CRP combined with chemotherapy agent increased the rate of immunogenic cell death and prevented melanoma growth". Oncology Letters 14.1 (2017): 844-852.
Chicago
Rodríguez‑Salazar, M. d., Franco‑Molina, M. A., Mendoza‑Gamboa, E., Martínez‑Torres, A. C., Zapata‑Benavides, P., López‑González, J. S., Coronado‑Cerda, E. E., Alcocer‑González, J. M., Tamez‑Guerra, R. S., Rodríguez‑Padilla, C."The novel immunomodulator IMMUNEPOTENT CRP combined with chemotherapy agent increased the rate of immunogenic cell death and prevented melanoma growth". Oncology Letters 14, no. 1 (2017): 844-852. https://doi.org/10.3892/ol.2017.6202
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Spandidos Publications style
Rodríguez‑Salazar Md, Franco‑Molina MA, Mendoza‑Gamboa E, Martínez‑Torres AC, Zapata‑Benavides P, López‑González JS, Coronado‑Cerda EE, Alcocer‑González JM, Tamez‑Guerra RS, Rodríguez‑Padilla C, Rodríguez‑Padilla C, et al: The novel immunomodulator IMMUNEPOTENT CRP combined with chemotherapy agent increased the rate of immunogenic cell death and prevented melanoma growth. Oncol Lett 14: 844-852, 2017.
APA
Rodríguez‑Salazar, M.d., Franco‑Molina, M.A., Mendoza‑Gamboa, E., Martínez‑Torres, A.C., Zapata‑Benavides, P., López‑González, J.S. ... Rodríguez‑Padilla, C. (2017). The novel immunomodulator IMMUNEPOTENT CRP combined with chemotherapy agent increased the rate of immunogenic cell death and prevented melanoma growth. Oncology Letters, 14, 844-852. https://doi.org/10.3892/ol.2017.6202
MLA
Rodríguez‑Salazar, M. d., Franco‑Molina, M. A., Mendoza‑Gamboa, E., Martínez‑Torres, A. C., Zapata‑Benavides, P., López‑González, J. S., Coronado‑Cerda, E. E., Alcocer‑González, J. M., Tamez‑Guerra, R. S., Rodríguez‑Padilla, C."The novel immunomodulator IMMUNEPOTENT CRP combined with chemotherapy agent increased the rate of immunogenic cell death and prevented melanoma growth". Oncology Letters 14.1 (2017): 844-852.
Chicago
Rodríguez‑Salazar, M. d., Franco‑Molina, M. A., Mendoza‑Gamboa, E., Martínez‑Torres, A. C., Zapata‑Benavides, P., López‑González, J. S., Coronado‑Cerda, E. E., Alcocer‑González, J. M., Tamez‑Guerra, R. S., Rodríguez‑Padilla, C."The novel immunomodulator IMMUNEPOTENT CRP combined with chemotherapy agent increased the rate of immunogenic cell death and prevented melanoma growth". Oncology Letters 14, no. 1 (2017): 844-852. https://doi.org/10.3892/ol.2017.6202
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