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Article Open Access

Impact of RASSF1A gene methylation on the metastatic axillary nodal status in breast cancer patients

  • Authors:
    • Eva Jezkova
    • Pavol Zubor
    • Karol Kajo
    • Marian Grendar
    • Karol Dokus
    • Marian Adamkov
    • Zora Lasabova
    • Lukas Plank
    • Jan Danko
  • View Affiliations / Copyright

    Affiliations: Department of Oncology, Biomedical Center Martin, Jessenius Faculty of Medicine, Comenius University in Bratislava, 036 01 Martin, Slovakia, St. Elizabeth Cancer Institute Hospital, 812 50 Bratislava, Slovakia, Bioinformatic Unit, Biomedical Center Martin, Jessenius Faculty of Medicine, Comenius University in Bratislava, 036 01 Martin, Slovakia, Department of Obstetrics and Gynaecology, Jessenius Faculty of Medicine, Martin University Hospital, 036 01 Martin, Slovakia, Department of Histology and Embryology, Jessenius Faculty of Medicine, Comenius University in Bratislava, 036 01 Martin, Slovakia, Department of Pathological Anatomy, Jessenius Faculty of Medicine, Martin University Hospital, 036 01 Martin, Slovakia
    Copyright: © Jezkova et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 758-766
    |
    Published online on: May 18, 2017
       https://doi.org/10.3892/ol.2017.6204
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Abstract

Hypermethylation of CpG islands is a hallmark of cancer and occurs at an early stage in breast tumorigenesis. To gain insight into the epigenetic switches that may promote and/or contribute to the initial neoplastic events during breast carcinogenesis, the present study focused on the DNA methylation profile of invasive breast carcinoma. The aim of the study was to evaluate the prognostic significance of Ras association domain family 1 isoform A (RASSF1A) promoter methylation status in operable breast cancer, and to analyze the utility of this biomarker regarding its association with metastatic and nonmetastatic axillary nodal status. For this purpose, formalin‑fixed, paraffin‑embedded tissue specimens from 116 breast cancer patients with known axillary nodal status were subjected to assessment of RASSF1A promoter methylation status by methylation‑specific polymerase chain reaction (MSP) and methylation‑sensitive high‑resolution melting assay, and the results were subsequently validated by bisulfite sequencing. A multinomial logistic regression model was used to model the dependence of distinct levels of methylation status of the RASSF1A promoter on the nodal status. Promoter region CpG hypermethylation was identified by MSP in 97 (83.6%) of 116 primary breast tumors, while hypermethylation of RASSF1A was confirmed by MS‑HRM in 107 (92.2%) of 116 cases of breast cancer. Based on the results of the multinomial logistic regression model, there was no significant difference between the frequency of RASSF1A promoter methylation and axillary lymph node status of patients in general. However, upon adjustment of pN stage, an association was identified between pN0 lymph node‑negative status (without axillary metastases) and percentage of RASSF1A methylation in two groups of heterogeneous methylated alleles with ≤50% methylated (P<0.05) and >50% methylated alleles (P<0.0001). If a patients' nodal status changes from pN‑ to pN+ then the risk of having >50% methylated alleles increases by 7%. The present study revealed a specific phenomenon, suggesting that the presence of heterogeneous methylated alleles in the RASSF1A gene is significantly associated with lymph node‑negative status in breast cancer patients. Furthermore, greater significance with negative axillary nodal status was observed with a higher level of heterogeneous methylated alleles in the RASSF1A gene.
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1 

Tao Z, Shi A, Lu C, Song T, Zhang Z and Zhao J: Breast cancer: epidemiology and etiology. Cell Biochem Biophys. 72:333–338. 2014. View Article : Google Scholar

2 

Lester SC and Hicks DG: Diagnostic Pathology: Breast. 2nd. Elsevier Health Sciences; 2016

3 

Lyman GH, Temin S, Edge BS, Newman LA, Turner RR, Weaver DL, Benson AB III, Bosserman LD, Burstein HJ, Cody H III, et al: Sentinel lymph node biopsy for patients with early-stage breast cancer: American Society of Clinical Oncology clinical practice guideline update. J Clin Oncol. 32:1365–1383. 2014. View Article : Google Scholar : PubMed/NCBI

4 

Weigelt B, Hu Z, He X, Livasy C, Carey LA, Ewend MG, Glas AM, Perou CM and Van't Veer LJ: Molecular portraits and 70-gene prognosis signature are preserved throughout the metastatic process of breast cancer. Cancer Res. 65:9155–9158. 2005. View Article : Google Scholar : PubMed/NCBI

5 

Weigelt B, Peterse JL and van 't Veer LJ: Breast cancer metastasis: Markers and models. Nat Rev Cancer. 5:591–602. 2005. View Article : Google Scholar : PubMed/NCBI

6 

Jones TI, Neboori H, Wu H, Yang Q, Haffty BG, Evans S, Higgins S and Moran MS: Are breast cancer subtypes prognostic for nodal involvement and associated with clinicopathologic features at presentation in early-stage breast cancer? Ann Surg Oncol. 20:2866–2872. 2013. View Article : Google Scholar : PubMed/NCBI

7 

Byler S, Goldgar S, Heerboth S, Leary M, Housman G, Moulton K and Sarkar S: Genetic and epigenetic aspects of breast cancer progression and therapy. Anticancer Res. 34:1071–1077. 2014.PubMed/NCBI

8 

Weigel MT and Dowsett M: Current and emerging biomarkers in breast cancer: Prognosis and prediction. Endocr Relat Cancer. 17:R245–R262. 2010. View Article : Google Scholar : PubMed/NCBI

9 

de Boer M, van Dijck JA, Bult P, Borm GF and Tjan-Heijnen VC: Breast cancer prognosis and occult lymph node metastases, isolated tumor cells, and micrometastases. J Natl Cancer Inst. 102:410–425. 2010. View Article : Google Scholar : PubMed/NCBI

10 

Yu M, Bardia A, Wittner BS, Stott SL, Smas ME, Ting DT, Isakoff SJ, Ciciliano JC, Wells MN, Shah AM, et al: Circulating breast tumor cells exhibit dynamic changes in epithelial and mesenchymal composition. Science. 339:580–584. 2013. View Article : Google Scholar : PubMed/NCBI

11 

Harlow SP and Weaver DL: Diagnosis, staging and the role of sentinel lymph node biopsy in the nodal evaluation of breast cancer. http://www.uptodate.com/contents/overview-of-sentinel-lymph-node-biopsy-in-breast-cancerDecember 12–2016

12 

Veta M, Pluim JP, Van Diest PJ and Viergever MA: Breast cancer histopathology image analysis: A review. IEEE Trans Biomed Eng. 61:1400–1411. 2014. View Article : Google Scholar : PubMed/NCBI

13 

Karakatsanis A, Christiansen PM, Fischer L, Hedin C, Pistioli L, Sund M, Rasmussen NR, Jørnsgård H, Tegnelius D, Eriksson S, et al: The Nordic SentiMag trial: A comparison of super paramagnetic iron oxide (SPIO) nanoparticles versus Tc(99) and patent blue in the detection of sentinel node (SN) in patients with breast cancer and a meta-analysis of earlier studies. Breast Cancer Res Treat. 157:281–294. 2016. View Article : Google Scholar : PubMed/NCBI

14 

Upender S, Mohan H, Hand U and Attri AK: Intraoperative evaluation of sentinel lymph nodes in breast carcinoma by imprint cytology, frozen section and rapid immunohistochemistry. Diagn Cytopathol. 37:871–875. 2009. View Article : Google Scholar : PubMed/NCBI

15 

Soares EW, Nagai HM, Bredt LC, da Cunha AD Jr, Andrade RJ and Soares GV: Morbidity after conventional dissection of axillary lymph nodes in breast cancer patients. World J Surg Oncol. 12:672014. View Article : Google Scholar : PubMed/NCBI

16 

Wang Z, Wu LC and Chen JQ: Sentinel lymph node biopsy compared with axillary lymph node dissection in early breast cancer: A meta-analysis. Breast Cancer Res Treat. 129:675–689. 2011. View Article : Google Scholar : PubMed/NCBI

17 

Gipponi M, Fregatti P, Garlaschi A, Murelli F, Margarino C, Depaoli F, Baccini P, Gallo M and Friedman D: Axillary ultrasound and Fine-Needle Aspiration Cytology in the preoperative staging of axillary node metastasis in breast cancer patients. Breast. 30:146–150. 2016. View Article : Google Scholar : PubMed/NCBI

18 

Co M and Kwong A: Preoperative Sentinel Node Mapping in Sentinel Node Biopsy in Early Breast Cancers-Is It Cost-Effective? Clin Breast Cancer. 2016.(Epub ahead of print). PubMed/NCBI

19 

Chirappapha P, Lohsiriwat V, Kongdan Y, Lertsithichai P, Sukarayothin T, Supsamutchai C, Talakhadze N and Zurrida S: Sentinel lymph node biopsy under local anesthesia in patients with breast cancer. Gland Surg. 1:151–155. 2012.PubMed/NCBI

20 

Luini A, Gatti G, Frasson A, Naninato P, Magalotti C, Arnone P, Viale G, Pruneri G, Galimberti V, De Cicco C and Veronesi U: Sentinel lymph node biopsy performed with local anesthesia in patients with early-stage breast carcinoma. Arch Surg. 137:1157–1160. 2002. View Article : Google Scholar : PubMed/NCBI

21 

Leidenius MH, Vironen JH, Heikkilä PS and Joensuu H: Influence of isolated tumor cells in sentinel nodes on outcome in small, node-negative (pT1N0M0) breast cancer. Ann Surg Oncol. 17:254–262. 2010. View Article : Google Scholar : PubMed/NCBI

22 

Gobardhan PD, Elias SG, Madsen EV, Bongers V, Ruitenberg HJ, Perre CI and Van Dalen T: Prognostic value of micrometastases in sentinel lymph nodes of patients with breast carcinoma: A cohort study. Ann Oncol. 20:41–48. 2009. View Article : Google Scholar : PubMed/NCBI

23 

Cul'bová M, Lasabová Z, Stanclová A, Tilandyová P, Zúbor P, Fiolka R, Danko J and Visnovský J: Methylation of selected tumor-supressor genes in benign and malignant ovarian tumors. Ceska Gynekol. 76:274–279. 2011.PubMed/NCBI

24 

Fiolka R, Zubor P, Janusicova V, Visnovsky J, Mendelova A, Kajo K, Lasabova Z, Plank L and Danko J: Promoter hypermethylation of the tumor-suppressor genes RASSF1A, GSTP1 and CDH1 in endometrial cancer. Oncol Rep. 30:2878–2886. 2013.PubMed/NCBI

25 

Alvarez C, Tapia T, Cornejo V, Fernandez W, Muñoz A, Camus M, Alvarez M, Devoto L and Carvallo P: Silencing of tumor suppressor genes RASSF1A, SLIT2 and WIF1 by promoter hypermethylation in hereditary breast cancer. Mol Carcinog. 52:475–487. 2013. View Article : Google Scholar : PubMed/NCBI

26 

Rauscher GH, Kresovich JK, Poulin M, Yan L, Macias V, Mahmoud AM, Al-Alem U, Kajdacsy-Balla A, Wiley EL, Tonetti D and Ehrlich M: Exploring DNA methylation changes in promoter, intragenic, and intergenic regions as early and late events in breast cancer formation. BMC Cancer. 15:8162015. View Article : Google Scholar : PubMed/NCBI

27 

Lakhani SR, Ellis IO, Schnitt SJ, Tan PH and van de Vijver MJ: WHO Classification of Tumours of the Breast. 4th. Lyon: IARC; 2012

28 

Elston CW and Ellis IO: Pathological prognostic factors in breast cancer. I. The value of histological grade in breast cancer: experience from a large study with long-term follow-up. Elston C.W..Ellis I. O.: Histopathology. 1991.19:403–410. Histopatology. 41:151–153. 2002. View Article : Google Scholar : PubMed/NCBI

29 

Edge SB and Compton CC: The American Joint Committee on Cancer: the 7th edition of the AJCC cancer staging manual and the future of TNM. Ann Surg Oncol. 17:1471–1474. 2010. View Article : Google Scholar : PubMed/NCBI

30 

Hammond ME, Haves DF, Wolff AC, Mangu PB and Temin S: American Society of Clinical Oncology/College of American Pathologists guideline recommendations for immunohistochemical testing of estrogen and progesterone receptors in breast cancer. J Oncol Pract. 6:195–197. 2010. View Article : Google Scholar : PubMed/NCBI

31 

Wolff AC, Hammond ME, Hicks DG, Dowsett M, McShane LM, Allison KH, Allred DC, Bartlett JM, Bilous M, Fitzgibbons P, et al: Recommendations for human epidermal growth factor receptor 2 testing in breast cancer: American Society of Clinical Oncology/College of American Pathologists clinical practice guideline update. Arch Pathol Lab Med. 138:241–256. 2014. View Article : Google Scholar : PubMed/NCBI

32 

Wolff AC, Hammond ME, Hicks DG, Dowsett M, McShane LM, Allison KH, Allred DC, Bartlett JM, Bilous M, Fitzgibbons P, et al: Recommendations for human epidermal growth factor receptor 2 testing in breast cancer: American Society of Clinical Oncology/College of American Pathologists clinical practice guideline update. J Clin Oncol. 31:3997–4013. 2013. View Article : Google Scholar : PubMed/NCBI

33 

Huang KT, Mikeska T, Li J, Takano EA, Millar EK, Graham PH, Boyle SE, Campbell IG, Speed TP, Dobrovic A and Fox SB: Assessment of DNA methylation profiling and copy number variation as indications of clonal relationship in ipsilateral and contralateral breast cancers to distinguish recurrent breast cancer from a second primary tumour. BMC Cancer. 15:6692015. View Article : Google Scholar : PubMed/NCBI

34 

Wojdacz TK and Dobrovic A: Methylation-sensitive high resolution melting (MS-HRM): A new approach for sensitive and high-throughput assessment of methylation. Nucleic Acids Res. 35:e412007. View Article : Google Scholar : PubMed/NCBI

35 

R Core Team. R, . A language and environment for statistical computing. R Foundation for Statistical Computing; Vienna, Austria: https://www.R-project.org/2015

36 

Dammann R, Schagdarsurengin U, Seidel C, Strunnikova M, Rastetter M, Baier K and Pfeifer GP: The tumor suppressor RASSF1A in human carcinogenesis: An update. Histol Histopathol. 20:645–663. 2005.PubMed/NCBI

37 

Agathanggelou A, Honorio S, Macartney DP, Martinez A, Dallol A, Rader J, Fullwood P, Chauhan A, Walker R, Shaw JA, et al: Methylation associated inactivation of RASSF1A from region 3p21. 3 in lung, breast and ovarian tumours. Oncogene. 20:1509–1518. 2001. View Article : Google Scholar : PubMed/NCBI

38 

Agathanggelou A, Cooper WN and Latif F: Role of the Ras-association domain family 1 tumor suppressor gene in human cancers. Cancer Res. 65:3497–3508. 2005. View Article : Google Scholar : PubMed/NCBI

39 

Dammann R, Yang G and Pfeifer GP: Hypermethylation of the cpG island of Ras association domain family 1A (RASSF1A), a putative tumor suppressor gene from the 3p21. 3 locus, occurs in a large percentage of human breast cancers. Cancer Res. 61:3105–3109. 2001.PubMed/NCBI

40 

Ghazaleh HA, Chow RS, Choo SL, Pham D, Olesen JD, Wong RX, Onyskiw C and Baksh S: 14-3-3 mediated regulation of the tumor suppressor protein, RASSF1A. Apoptosis. 15:117–127. 2010. View Article : Google Scholar : PubMed/NCBI

41 

Shivakumar L, Minna J, Sakamaki T, Pestell R and White MA: The RASSF1A tumor suppressor blocks cell cycle progression and inhibits cyclin D1 accumulation. Mol Cell Biol. 22:4309–4318. 2002. View Article : Google Scholar : PubMed/NCBI

42 

Dallol A, Agathanggelou A, Fenton SL, Ahmed-Choudhury J, Hesson L, Vos MD, Clark GJ, Downward J, Maher ER and Latif F: RASSF1A interacts with microtubule-associated proteins and modulates microtubule dynamics. Cancer Res. 64:4112–4116. 2004. View Article : Google Scholar : PubMed/NCBI

43 

Duffy MJ, Sturgeon CM, Sölétormos G, Barak V, Molina R, Hayes DF, Diamandis EP and Bossuyt PM: Validation of new cancer biomarkers: A position statement from the European group on tumor markers. Clin Chem. 61:809–820. 2015. View Article : Google Scholar : PubMed/NCBI

44 

Lewis CM, Cler LR, Bu DW, Zöchbauer-Müller S, Milchgrub S, Naftalis EZ, Leitch AM, Minna JD and Euhus DM: Promoter hypermethylation in benign breast epithelium in relation to predicted breast cancer risk. Clin Cancer Res. 11:166–172. 2005.PubMed/NCBI

45 

Müller HM, Widschwendter A, Fiegl H, Ivarsson L, Goebel G, Perkmann E, Marth C and Widschwendter M: DNA methylation in serum of breast cancer patients: An independent prognostic marker. Cancer Res. 63:7641–7645. 2003.PubMed/NCBI

46 

Marino AL, Evangelista AF, Vieira RA, Macedo T, Kerr LM, Abrahão-Machado LF, Longatto-Filho A, Silveira HC and Marques MM: MicroRNA expression as risk biomarker of breast cancer metastasis: A pilot retrospective case-cohort study. BMC Cancer. 14:7392014. View Article : Google Scholar : PubMed/NCBI

47 

Sequeiros T, García M, Montes M, Oliván M, Rigau M, Colás E, De Torres I, Morote J, Reventós J and Doll A: Molecular markers for prostate cancer in formalin-fixed paraffin-embedded tissues. Biomed Res Int. 2013:2836352013. View Article : Google Scholar : PubMed/NCBI

48 

van Hoesel AQ, Sato Y, Elashoff DA, Turner RR, Giuliano AE, Shamonki JM, Kuppen PJ, van de Velde CJ and Hoon DS: Assessment of DNA methylation status in early stages of breast cancer development. Br J Cancer. 108:2033–2038. 2013. View Article : Google Scholar : PubMed/NCBI

49 

Schrijver WA, Jiwa LS, van Diest PJ and Moelans CB: Promoter hypermethylation profiling of distant breast cancer metastases. Breast Cancer Res Treat. 151:41–55. 2015. View Article : Google Scholar : PubMed/NCBI

50 

Fackler MJ, McVeigh M, Evron E, Garrett E, Mehrotra J, Polyak K, Sukumar S and Argani P: DNA methylation of RASSF1A, HIN-1, RAR-β, Cyclin D2 and Twist in in situ and invasive lobular breast carcinoma. Int J Cancer. 107:970–975. 2003. View Article : Google Scholar : PubMed/NCBI

51 

Fackler MJ, McVeigh M, Mehrotra J, Blum MA, Lange J, Lapides A, Garrett E, Argani P and Sukumar S: Quantitative multiplex methylation-specific PCR assay for the detection of promoter hypermethylation in multiple genes in breast cancer. Cancer Res. 64:4442–4452. 2004. View Article : Google Scholar : PubMed/NCBI

52 

Swift-Scanlan T, Vang R, Blackford A, Fackler MJ and Sukumar S: Methylated genes in breast cancer: Associations with clinical and histopathological features in a familial breast cancer cohort. Cancer Biol Ther. 11:853–865. 2011. View Article : Google Scholar : PubMed/NCBI

53 

Feng W, Orlandi R, Zhao N, Carcangiu ML, Tagliabue E, Xu J, Bast RC Jr and Yu Y: Tumor suppressor genes are frequently methylated in lymph node metastases of breast cancers. BMC Cancer. 10:3782010. View Article : Google Scholar : PubMed/NCBI

54 

Rouzier R, Extra JM, Klijanienko J, Falcou MC, Asselain B, Vincent-Salomon A, Vielh P and Bourstyn E: Incidence and prognostic significance of complete axillary downstaging after primary chemotherapy in breast cancer patients with T1 to T3 tumors and cytologically proven axillary metastatic lymph nodes. J Clin Oncol. 20:1304–1310. 2002. View Article : Google Scholar : PubMed/NCBI

55 

Fisher ER, Anderson S, Redmond C and Fisher B: Pathologic findings from the national surgical adjuvant breast project protocol B-06: 10-year pathologic and clinical prognostic discriminants. Cancer. 71:2507–2514. 1993. View Article : Google Scholar : PubMed/NCBI

56 

Fitzgibbons PL, Page DL, Weaver D, Thor AD, Allred DC, Clark GM, Ruby SG, O'Malley F, Simpson JF, Connolly JL, et al: Prognostic factors in breast cancer: College of American Pathologists consensus statement 1999. Arch Pathol Lab Med. 124:966–978. 2000.PubMed/NCBI

57 

Jatoi I, Hilsenbeck SG, Clark GM and Osborne CK: Significance of axillary lymph node metastasis in primary breast cancer. J Clin Oncol. 17:2334–2340. 1999. View Article : Google Scholar : PubMed/NCBI

58 

Weiss M, Meyer M, Siegert S, Bartenstein P and Pfluger T: Metastases in patients with breast cancer despite of negative sentinel lymph node. Has the concept to be changed? Nuklearmedizin. 52:14–20. 2013.PubMed/NCBI

59 

Wen HY, Krystel-Whittemore M, Patil S, Pareja F, Bowser ZL, Dickler MN, Norton L, Morrow M, Hudis CA and Brogi E: Breast carcinoma with an Oncotype Dx recurrence score< 18: Rate of distant metastases in a large series with clinical follow-up. Cancer. 123:131–137. 2017. View Article : Google Scholar : PubMed/NCBI

60 

Wojdacz TK, Møller TH, Thestrup BB, Kristensen LS and Hansen LL: Limitations and advantages of MS-HRM and bisulfite sequencing for single locus methylation studies. Expert Rev Mol Diagn. 10:575–580. 2010. View Article : Google Scholar : PubMed/NCBI

61 

Wojdacz TK, Windeløv JA, Thestrup BB, Damsgaard TE, Overgaard J and Hansen L: Identification and characterization of locus-specific methylation patterns within novel loci undergoing hypermethylation during breast cancer pathogenesis. Breast Cancer Res. 16:R172014. View Article : Google Scholar : PubMed/NCBI

62 

Dobrovic A: Analysis of DNA methylation in clinical samples: Methods and applicationsmolecular pathology in cancer research. pp. 261–277. Springer; New York: 2016, View Article : Google Scholar

63 

Galimberti V, Cole BF, Zurrida S, Viale G, Luini A, Veronesi P, Baratella P, Chifu C, Sargenti M, Intra M, et al: Axillary dissection versus no axillary dissection in patients with sentinel-node micrometastases (IBCSG 23-01): A phase 3 randomised controlled trial. Lancet Oncol. 14:297–305. 2013. View Article : Google Scholar : PubMed/NCBI

64 

Giuliano AE, Ballman K, McCall L, Beitsch P, Whitworth PW, Blumencranz P, Leitch AM, Saha S, Morrow M and Hunt KK: Locoregional Recurrence After Sentinel Lymph Node Dissection With or Without Axillary Dissection in Patients With Sentinel Lymph Node Metastases: Long-term Follow-up From the American College of Surgeons Oncology Group (Alliance) ACOSOG Z0011 Randomized Trial. Ann Surg. 264:413–420. 2016. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Jezkova E, Zubor P, Kajo K, Grendar M, Dokus K, Adamkov M, Lasabova Z, Plank L and Danko J: Impact of RASSF1A gene methylation on the metastatic axillary nodal status in breast cancer patients. Oncol Lett 14: 758-766, 2017.
APA
Jezkova, E., Zubor, P., Kajo, K., Grendar, M., Dokus, K., Adamkov, M. ... Danko, J. (2017). Impact of RASSF1A gene methylation on the metastatic axillary nodal status in breast cancer patients. Oncology Letters, 14, 758-766. https://doi.org/10.3892/ol.2017.6204
MLA
Jezkova, E., Zubor, P., Kajo, K., Grendar, M., Dokus, K., Adamkov, M., Lasabova, Z., Plank, L., Danko, J."Impact of RASSF1A gene methylation on the metastatic axillary nodal status in breast cancer patients". Oncology Letters 14.1 (2017): 758-766.
Chicago
Jezkova, E., Zubor, P., Kajo, K., Grendar, M., Dokus, K., Adamkov, M., Lasabova, Z., Plank, L., Danko, J."Impact of RASSF1A gene methylation on the metastatic axillary nodal status in breast cancer patients". Oncology Letters 14, no. 1 (2017): 758-766. https://doi.org/10.3892/ol.2017.6204
Copy and paste a formatted citation
x
Spandidos Publications style
Jezkova E, Zubor P, Kajo K, Grendar M, Dokus K, Adamkov M, Lasabova Z, Plank L and Danko J: Impact of RASSF1A gene methylation on the metastatic axillary nodal status in breast cancer patients. Oncol Lett 14: 758-766, 2017.
APA
Jezkova, E., Zubor, P., Kajo, K., Grendar, M., Dokus, K., Adamkov, M. ... Danko, J. (2017). Impact of RASSF1A gene methylation on the metastatic axillary nodal status in breast cancer patients. Oncology Letters, 14, 758-766. https://doi.org/10.3892/ol.2017.6204
MLA
Jezkova, E., Zubor, P., Kajo, K., Grendar, M., Dokus, K., Adamkov, M., Lasabova, Z., Plank, L., Danko, J."Impact of RASSF1A gene methylation on the metastatic axillary nodal status in breast cancer patients". Oncology Letters 14.1 (2017): 758-766.
Chicago
Jezkova, E., Zubor, P., Kajo, K., Grendar, M., Dokus, K., Adamkov, M., Lasabova, Z., Plank, L., Danko, J."Impact of RASSF1A gene methylation on the metastatic axillary nodal status in breast cancer patients". Oncology Letters 14, no. 1 (2017): 758-766. https://doi.org/10.3892/ol.2017.6204
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