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Article Open Access

pPeOp from Omphalia lapidescens Schroet induces cell cycle arrest and inhibits the migration of MC‑4 gastric tumor cells

  • Authors:
    • Yong‑Le Yang
    • Wei‑Yao Gong
    • Fei‑Fei Chen
    • Lu‑Chao Chen
    • Yi‑Tao Chen
  • View Affiliations / Copyright

    Affiliations: College of Life Sciences, Zhejiang Chinese Medical University, Hangzhou, Zhejiang 310053, P.R. China
    Copyright: © Yang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 533-540
    |
    Published online on: May 19, 2017
       https://doi.org/10.3892/ol.2017.6207
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Abstract

The aim of the present study was to investigate the effect of purified Omphalia lapidescens protein (pPeOp) extracted by polyvinylpyrrolidone from the fungus Omphalia lapidescens Schroet on the proliferation and cell cycle progression of MC‑4 human gastric tumor cells. Using polyvinylpyrrolidone, pPeOp was extracted from O. lapidescens Schroet. MC‑4 cells were cultured with 30, 60 or 90 µg/ml pPeOp, with 5‑fluorouracil used as a positive control. Survival rates of treated cells were significantly decreased compared with those of the untreated control group in a dose‑dependent manner. Using flow cytometric analysis, cells treated with pPeOp were demonstrated to arrest in S phase and exhibit abnormal G0/G1 and G2/M phase cell cycle distribution. In addition, a wound healing assay demonstrated that pPeOp significantly inhibited the migration of MC‑4 cells. The mRNA and protein expression levels of cyclin D1/cyclin‑dependent kinase (CDK) 4, cyclin B/CDK1, cyclin A/CDK2, matrix metalloproteinase (MMP)‑2 and MMP‑9 were determined using reverse transcription‑quantitative polymerase chain reaction analysis and western blotting. The mRNA expression level of CDK4 and cyclin A was significantly increased compared with the untreated control; however, cyclin D1, CDK1, CDK2, cyclin B, MMP‑2, and MMP‑9 exhibited a significantly decreased mRNA expression level, indicating that there is a negative association between concentration and cyclin D1 expression levels. The expression of the cycle arrest‑associated proteins and migration‑associated proteins examined were similar to the observed mRNA expression levels. In conclusion, pPeOp was identified to inhibit migration of and cause S phase cell cycle arrest in MC‑4 cells.
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1 

Song W, Liu YY, Peng JJ, Liang HH, Chen HY, Chen JH, He WL, Xu JB, Cai SR and He YL: Identification of differentially expressed signatures of long non-coding RNAs associated with different metastatic potentials in gastric cancer. J Gastroenterol. 51:119–129. 2016. View Article : Google Scholar : PubMed/NCBI

2 

Choi HJ, Ki CS, Suh SP and Kim JW: Presymptomatic identification of CDH1 germline mutation in a healthy Korean individual with family history of gastric cancer. Ann Lab Med. 34:386–389. 2014. View Article : Google Scholar : PubMed/NCBI

3 

Jiang C, Chen X, Alattar M, Wei J and Liu H: MicroRNAs in tumorigenesis, metastasis, diagnosis and prognosis of gastric cancer. Cancer Gene Ther. 22:291–301. 2015. View Article : Google Scholar : PubMed/NCBI

4 

Zhao J, Liu Y, Huang G, Cui P, Zhang W and Zhang Y: Long non-coding RNAs in gastric cancer: Versatile mechanisms and potential for clinical translation. Am J Cancer Res. 5:907–927. 2015.PubMed/NCBI

5 

Robertson-Tessi M, Gillies RJ, Gatenby RA and Anderson AR: Impact of metabolic heterogeneity on tumor growth, invasion, and treatment outcomes. Cancer Res. 75:1567–1579. 2015. View Article : Google Scholar : PubMed/NCBI

6 

Li J, Li L, Liu R and Lin HS: Establishing Chinese medicine characteristic tumor response evaluation system is the key to promote internationalization of Chinese medicine oncology. Chin J Integr Med. 18:730–736. 2012. View Article : Google Scholar : PubMed/NCBI

7 

Qi F, Li A, Inagaki Y, Gao J, Li J, Kokudo N, Li XK and Tang W: Chinese herbal medicines as adjuvant treatment during chemo- or radio-therapy for cancer. Biosci Trends. 4:297–307. 2010.PubMed/NCBI

8 

Ling CQ, Yue XQ and Ling C: Three advantages of using traditional Chinese medicine to prevent and treat tumor. J Integr Med. 12:331–335. 2014. View Article : Google Scholar : PubMed/NCBI

9 

Chen YT, Lin MA, Cheng DQ, Shi ZJ, Zhu JL and Wu J: Effect of proteins extracted from mycelia of Omphalia lapidescens on inhibiting H, liver cancer in mice and regulating immune function. Zhong Yao Cai. 32:1870–1874. 2009.(In Chinese). PubMed/NCBI

10 

Yan H, Rong X, Chen PT, Zhang X and Ma ZQ: Two new steroids from sclerotia of the fungus Omphalia lapidescens. J Asian Nat Prod Res. 16:265–270. 2014. View Article : Google Scholar : PubMed/NCBI

11 

Ohno N, Saito K, Nemoto J, Kaneko S, Adachi Y, Nishijima M, Miyazaki T and Yadomae T: Immunopharmacological characterization of a highly branched fungal (1->3)-beta-D-glucan, OL-2, isolated from Omphalia lapidescens. Biol Pharm Bull. 16:414–419. 1993. View Article : Google Scholar : PubMed/NCBI

12 

Saito K, Nishijima M, Ohno N, Yadomae T and Miyazaki T: Structure and antitumor activity of the less-branched derivatives of an alkali-soluble glucan isolated from Omphalia lapidescens. (Studies on fungal polysaccharide. XXXVIII). Chem Pharm Bull (Tokyo). 40:261–263. 1992. View Article : Google Scholar : PubMed/NCBI

13 

Zhang G, Huang Y, Bian Y, Wong JH, Ng TB and Wang H: Hypoglycemic activity of the fungi Cordyceps militarisCordyceps sinensisTricholoma mongolicum, and Omphalia lapidescens in streptozotocin-induced diabetic rats. Appl Microbiol Biotechnol. 72:1152–1156. 2006. View Article : Google Scholar : PubMed/NCBI

14 

Chen YT, Lu QY, Lin MA, Cheng DQ, Ding ZS and Shan LT: A PVP-extract fungal protein of Omphalia lapidescens and its antitumor activity on human gastric tumors and normal cells. Oncol Rep. 26:1519–1526. 2011.PubMed/NCBI

15 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(−Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

16 

Zhang Y, Pan T, Zhong X and Cheng C: Androgen receptor promotes esophageal cancer cell migration and proliferation via matrix metalloproteinase 2. Tumour Biol. 36:5859–5864. 2015. View Article : Google Scholar : PubMed/NCBI

17 

Lin Y, Peng N, Zhuang H, Zhang D, Wang Y and Hua ZC: Heat shock proteins HSP70 and MRJ cooperatively regulate cell adhesion and migration through urokinase receptor. BMC Cancer. 14:6392014. View Article : Google Scholar : PubMed/NCBI

18 

Sun A, Yu G, Dou X, Yan X, Yang W and Lin Q: Nedd4-1 is an exceptional prognostic biomarker for gastric cardia adenocarcinoma and functionally associated with metastasis. Mol Cancer. 13:2482014. View Article : Google Scholar : PubMed/NCBI

19 

Cao K, Xie D, Cao P, Zou Q, Lu C, Xiao S, Zhou J and Peng X: SiRNA-mediated flotillin-2 (Flot2) downregulation inhibits cell proliferation, migration, and invasion in gastric carcinoma cells. Oncol Res. 21:271–279. 2014. View Article : Google Scholar : PubMed/NCBI

20 

Lu S, Zhu Q, Zhang Y, Song W, Wilson MJ and Liu P: Dual-functions of miR-373 and miR-520c by differently regulating the activities of MMP2 and MMP9. J Cell Physiol. 230:1862–1870. 2015. View Article : Google Scholar : PubMed/NCBI

21 

Tong WW, Tong GH, Chen XX, Zheng HC and Wang YZ: HIF2α is associated with poor prognosis and affects the expression levels of survivin and cyclin D1 in gastric carcinoma. Int J Oncol. 46:233–242. 2015.PubMed/NCBI

22 

Płuciennik E, Nowakowska M, Pospiech K, Stępień A, Wołkowicz M, Gałdyszyńska M, Popęda M, Wójcik-Krowiranda K, Bieńkiewicz A and Bednarek AK: The role of WWOX tumor suppressor gene in the regulation of EMT process via regulation of CDH1-ZEB1-VIM expression in endometrial cancer. Int J Oncol. 46:2639–2648. 2015.PubMed/NCBI

23 

Leight JL, Tokuda EY, Jones CE, Lin AJ and Anseth KS: Multifunctional bioscaffolds for 3D culture of melanoma cells reveal increased MMP activity and migration with BRAF kinase inhibition. Proc Natl Acad Sci USA. 112:pp. 5366–5371. 2015; View Article : Google Scholar : PubMed/NCBI

24 

Zheng L, Zhang YM, Zhan YZ and Liu CX: Momordica cochinchinensis seed extracts suppress migration and invasion of human breast cancer ZR-75-30 cells via down-regulating MMP-2 and MMP-9. Asian Pac J Cancer Prev. 15:1105–1110. 2014. View Article : Google Scholar : PubMed/NCBI

25 

da Rosa MR, Falcão AS, Fuzii HT, da Silva Kataoka MS, Ribeiro AL, Boccardo E, De Siqueira AS, Jaeger RG, de Jesus Viana Pinheiro J and de Melo Alves S Júnior: EGFR signaling downstream of EGF regulates migration, invasion and MMP secretion of immortalized cells derived from human ameloblastoma. Tumour Biol. 35:11107–11120. 2014. View Article : Google Scholar : PubMed/NCBI

26 

Liu F, Zhou J, Zhou P, Chen W and Guo F: The ubiquitin ligase CHIP inactivates NF-κB signaling and impairs the ability of migration and invasion in gastric cancer cells. Int J Oncol. 46:2096–2106. 2015.PubMed/NCBI

27 

Kim D, Lee D, Jang YL, Chae SY, Choi D, Jeong JH and Kim SH: Facial amphipathic deoxycholic acid-modified polyethyleneimine for efficient MMP-2 siRNA delivery in vascular smooth muscle cells. Eur J Pharm Biopharm. 81:14–23. 2012. View Article : Google Scholar : PubMed/NCBI

28 

Matusewicz L, Meissner J, Toporkiewicz M and Sikorski AF: The effect of statins on cancer cells-review. Tumour Biol. 36:4889–4904. 2015. View Article : Google Scholar : PubMed/NCBI

29 

Parajuli KR, Zhang Q, Liu S and You Z: Aminomethylphosphonic acid and methoxyacetic acid induce apoptosis in prostate cancer cells. Int J Mol Sci. 16:11750–11765. 2015. View Article : Google Scholar : PubMed/NCBI

30 

Saiko P, Steinmann MT, Schuster H, Graser G, Bressler S, Giessrigl B, Lackner A, Grusch M, Krupitza G, Bago-Horvath Z, et al: Epigallocatechin gallate, ellagic acid, and rosmarinic acid perturb dNTP pools and inhibit de novo DNA synthesis and proliferation of human HL-60 promyelocytic leukemia cells: Synergism with arabinofuranosylcytosine. Phytomedicine. 22:213–222. 2015. View Article : Google Scholar : PubMed/NCBI

31 

Hong S, Gu Y, Gao Z, Guo L, Guo W, Wu X, Shen Y, Sun Y, Wu X and Xu Q: EGFR inhibitor-driven endoplasmic reticulum stress-mediated injury on intestinal epithelial cells. Life Sci. 119:28–33. 2014. View Article : Google Scholar : PubMed/NCBI

32 

Evers B, Helleday T and Jonkers J: Targeting homologous recombination repair defects in cancer. Trends Pharmacol Sci. 31:372–380. 2010. View Article : Google Scholar : PubMed/NCBI

33 

Liu Z, Rader J, He S, Phung T and Thiele CJ: CASZ1 inhibits cell cycle progression in neuroblastoma by restoring pRb activity. Cell Cycle. 12:2210–2218. 2013. View Article : Google Scholar : PubMed/NCBI

34 

Cao Z, Lin W, Huang Z, Chen X, Zhao J, Zheng L, Ye H, Liu Z, Liao L and Du J: Ethyl acetate extraction from a Chinese herbal formula, Jiedu Xiaozheng Yin, inhibits the proliferation of hepatocellular carcinoma cells via induction of G0/G1 phase arrest in vivo and in vitro. Int J Oncol. 42:202–210. 2013.PubMed/NCBI

35 

Marconett CN, Morgenstern TJ, San Roman AK, Sundar SN, Singhal AK and Firestone GL: BZL101, a phytochemical extract from the Scutellaria barbata plant, disrupts proliferation of human breast and prostate cancer cells through distinct mechanisms dependent on the cancer cell phenotype. Cancer Biol Ther. 10:397–405. 2010. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Yang YL, Gong WY, Chen FF, Chen LC and Chen YT: pPeOp from Omphalia lapidescens Schroet induces cell cycle arrest and inhibits the migration of MC‑4 gastric tumor cells. Oncol Lett 14: 533-540, 2017.
APA
Yang, Y., Gong, W., Chen, F., Chen, L., & Chen, Y. (2017). pPeOp from Omphalia lapidescens Schroet induces cell cycle arrest and inhibits the migration of MC‑4 gastric tumor cells. Oncology Letters, 14, 533-540. https://doi.org/10.3892/ol.2017.6207
MLA
Yang, Y., Gong, W., Chen, F., Chen, L., Chen, Y."pPeOp from Omphalia lapidescens Schroet induces cell cycle arrest and inhibits the migration of MC‑4 gastric tumor cells". Oncology Letters 14.1 (2017): 533-540.
Chicago
Yang, Y., Gong, W., Chen, F., Chen, L., Chen, Y."pPeOp from Omphalia lapidescens Schroet induces cell cycle arrest and inhibits the migration of MC‑4 gastric tumor cells". Oncology Letters 14, no. 1 (2017): 533-540. https://doi.org/10.3892/ol.2017.6207
Copy and paste a formatted citation
x
Spandidos Publications style
Yang YL, Gong WY, Chen FF, Chen LC and Chen YT: pPeOp from Omphalia lapidescens Schroet induces cell cycle arrest and inhibits the migration of MC‑4 gastric tumor cells. Oncol Lett 14: 533-540, 2017.
APA
Yang, Y., Gong, W., Chen, F., Chen, L., & Chen, Y. (2017). pPeOp from Omphalia lapidescens Schroet induces cell cycle arrest and inhibits the migration of MC‑4 gastric tumor cells. Oncology Letters, 14, 533-540. https://doi.org/10.3892/ol.2017.6207
MLA
Yang, Y., Gong, W., Chen, F., Chen, L., Chen, Y."pPeOp from Omphalia lapidescens Schroet induces cell cycle arrest and inhibits the migration of MC‑4 gastric tumor cells". Oncology Letters 14.1 (2017): 533-540.
Chicago
Yang, Y., Gong, W., Chen, F., Chen, L., Chen, Y."pPeOp from Omphalia lapidescens Schroet induces cell cycle arrest and inhibits the migration of MC‑4 gastric tumor cells". Oncology Letters 14, no. 1 (2017): 533-540. https://doi.org/10.3892/ol.2017.6207
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