Open Access

pPeOp from Omphalia lapidescens Schroet induces cell cycle arrest and inhibits the migration of MC‑4 gastric tumor cells

  • Authors:
    • Yong‑Le Yang
    • Wei‑Yao Gong
    • Fei‑Fei Chen
    • Lu‑Chao Chen
    • Yi‑Tao Chen
  • View Affiliations

  • Published online on: May 19, 2017     https://doi.org/10.3892/ol.2017.6207
  • Pages: 533-540
  • Copyright: © Yang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The aim of the present study was to investigate the effect of purified Omphalia lapidescens protein (pPeOp) extracted by polyvinylpyrrolidone from the fungus Omphalia lapidescens Schroet on the proliferation and cell cycle progression of MC‑4 human gastric tumor cells. Using polyvinylpyrrolidone, pPeOp was extracted from O. lapidescens Schroet. MC‑4 cells were cultured with 30, 60 or 90 µg/ml pPeOp, with 5‑fluorouracil used as a positive control. Survival rates of treated cells were significantly decreased compared with those of the untreated control group in a dose‑dependent manner. Using flow cytometric analysis, cells treated with pPeOp were demonstrated to arrest in S phase and exhibit abnormal G0/G1 and G2/M phase cell cycle distribution. In addition, a wound healing assay demonstrated that pPeOp significantly inhibited the migration of MC‑4 cells. The mRNA and protein expression levels of cyclin D1/cyclin‑dependent kinase (CDK) 4, cyclin B/CDK1, cyclin A/CDK2, matrix metalloproteinase (MMP)‑2 and MMP‑9 were determined using reverse transcription‑quantitative polymerase chain reaction analysis and western blotting. The mRNA expression level of CDK4 and cyclin A was significantly increased compared with the untreated control; however, cyclin D1, CDK1, CDK2, cyclin B, MMP‑2, and MMP‑9 exhibited a significantly decreased mRNA expression level, indicating that there is a negative association between concentration and cyclin D1 expression levels. The expression of the cycle arrest‑associated proteins and migration‑associated proteins examined were similar to the observed mRNA expression levels. In conclusion, pPeOp was identified to inhibit migration of and cause S phase cell cycle arrest in MC‑4 cells.
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July-2017
Volume 14 Issue 1

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Online ISSN:1792-1082

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Spandidos Publications style
Yang YL, Gong WY, Chen FF, Chen LC and Chen YT: pPeOp from Omphalia lapidescens Schroet induces cell cycle arrest and inhibits the migration of MC‑4 gastric tumor cells. Oncol Lett 14: 533-540, 2017
APA
Yang, Y., Gong, W., Chen, F., Chen, L., & Chen, Y. (2017). pPeOp from Omphalia lapidescens Schroet induces cell cycle arrest and inhibits the migration of MC‑4 gastric tumor cells. Oncology Letters, 14, 533-540. https://doi.org/10.3892/ol.2017.6207
MLA
Yang, Y., Gong, W., Chen, F., Chen, L., Chen, Y."pPeOp from Omphalia lapidescens Schroet induces cell cycle arrest and inhibits the migration of MC‑4 gastric tumor cells". Oncology Letters 14.1 (2017): 533-540.
Chicago
Yang, Y., Gong, W., Chen, F., Chen, L., Chen, Y."pPeOp from Omphalia lapidescens Schroet induces cell cycle arrest and inhibits the migration of MC‑4 gastric tumor cells". Oncology Letters 14, no. 1 (2017): 533-540. https://doi.org/10.3892/ol.2017.6207