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Transforming growth factor‑β1 contributes to oxaliplatin resistance in colorectal cancer via epithelial to mesenchymal transition

  • Authors:
    • Liang Mao
    • Yan Li
    • Jinping Zhao
    • Qia Li
    • Bin Yang
    • Yuanyuan Wang
    • Zhitu Zhu
    • Hongzhi Sun
    • Zhenhua Zhai
  • View Affiliations / Copyright

    Affiliations: Department of Oncology, The First Affiliated Hospital of Liaoning Medical University, Jinzhou, Liaoning 121000, P.R. China, Tumor Angiogenesis and Microenvironment Laboratory (TAML), The First Affiliated Hospital of Liaoning Medical University, Jinzhou, Liaoning 121000, P.R. China
    Copyright: © Mao et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 647-654
    |
    Published online on: May 19, 2017
       https://doi.org/10.3892/ol.2017.6209
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Abstract

Transforming growth factor‑β1 (TGF‑β1), secreted by main components of tumor microenvironment, is considered to be closely associated with cancer development and chemoresistance. The present study aimed to analyze the effects and mechanisms underlying TGF‑β1‑induced chemoresistance to oxaliplatin (LOH) in human colorectal cancer (CRC) cell lines. The cytotoxic effects of LOH subsequent to TGF‑β1 treatment were assessed in three CRC cell lines by MTT assay. In addition, epithelial to mesenchymal transition (EMT), DNA damage and apoptosis assays were performed to evaluate the mechanisms of drug resistance in vitro. It was revealed that an exposure of CRC cells to TGF‑β1 induced EMT. This was followed by a decrease in the levels of DNA damage and LOH‑induced apoptotic cell death at certain TGF‑β1 concentrations compared with untreated cells, which was responsible for LOH resistance. TGF‑β1 leads to resistance to LOH in CRC cells, primarily through EMT. These data not only provide insight into the understanding of the chemoresistant mechanisms, but also may guide the clinical applications of reducing EMT to enhance the sensitivity to chemotherapy, by targeting TGF‑β1.
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Copy and paste a formatted citation
Spandidos Publications style
Mao L, Li Y, Zhao J, Li Q, Yang B, Wang Y, Zhu Z, Sun H and Zhai Z: Transforming growth factor‑β1 contributes to oxaliplatin resistance in colorectal cancer via epithelial to mesenchymal transition. Oncol Lett 14: 647-654, 2017.
APA
Mao, L., Li, Y., Zhao, J., Li, Q., Yang, B., Wang, Y. ... Zhai, Z. (2017). Transforming growth factor‑β1 contributes to oxaliplatin resistance in colorectal cancer via epithelial to mesenchymal transition. Oncology Letters, 14, 647-654. https://doi.org/10.3892/ol.2017.6209
MLA
Mao, L., Li, Y., Zhao, J., Li, Q., Yang, B., Wang, Y., Zhu, Z., Sun, H., Zhai, Z."Transforming growth factor‑β1 contributes to oxaliplatin resistance in colorectal cancer via epithelial to mesenchymal transition". Oncology Letters 14.1 (2017): 647-654.
Chicago
Mao, L., Li, Y., Zhao, J., Li, Q., Yang, B., Wang, Y., Zhu, Z., Sun, H., Zhai, Z."Transforming growth factor‑β1 contributes to oxaliplatin resistance in colorectal cancer via epithelial to mesenchymal transition". Oncology Letters 14, no. 1 (2017): 647-654. https://doi.org/10.3892/ol.2017.6209
Copy and paste a formatted citation
x
Spandidos Publications style
Mao L, Li Y, Zhao J, Li Q, Yang B, Wang Y, Zhu Z, Sun H and Zhai Z: Transforming growth factor‑β1 contributes to oxaliplatin resistance in colorectal cancer via epithelial to mesenchymal transition. Oncol Lett 14: 647-654, 2017.
APA
Mao, L., Li, Y., Zhao, J., Li, Q., Yang, B., Wang, Y. ... Zhai, Z. (2017). Transforming growth factor‑β1 contributes to oxaliplatin resistance in colorectal cancer via epithelial to mesenchymal transition. Oncology Letters, 14, 647-654. https://doi.org/10.3892/ol.2017.6209
MLA
Mao, L., Li, Y., Zhao, J., Li, Q., Yang, B., Wang, Y., Zhu, Z., Sun, H., Zhai, Z."Transforming growth factor‑β1 contributes to oxaliplatin resistance in colorectal cancer via epithelial to mesenchymal transition". Oncology Letters 14.1 (2017): 647-654.
Chicago
Mao, L., Li, Y., Zhao, J., Li, Q., Yang, B., Wang, Y., Zhu, Z., Sun, H., Zhai, Z."Transforming growth factor‑β1 contributes to oxaliplatin resistance in colorectal cancer via epithelial to mesenchymal transition". Oncology Letters 14, no. 1 (2017): 647-654. https://doi.org/10.3892/ol.2017.6209
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