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Article

Epigallocatechin-3-gallate inhibits growth and induces apoptosis in esophageal cancer cells through the demethylation and reactivation of the p16 gene

  • Authors:
    • Jianchao Meng
    • Qiang Tong
    • Xiaobo Liu
    • Zongtao Yu
    • Jicai Zhang
    • Bo Gao
  • View Affiliations / Copyright

    Affiliations: Department of Oncology, Taihe Hospital, Hubei University of Medicine, Shiyan, Hubei 442000, P.R. China, Department of Gastroenterology, Taihe Hospital, Hubei University of Medicine, Shiyan, Hubei 442000, P.R. China, Department of Laboratory Medicine, Taihe Hospital, Hubei University of Medicine, Shiyan, Hubei 442000, P.R. China
  • Pages: 1152-1156
    |
    Published online on: May 25, 2017
       https://doi.org/10.3892/ol.2017.6248
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Abstract

The present study aimed to investigate the effect of treatment with epigallocatechin-3-gallate (EGCG) on the human esophageal cancer cell line ECa109 and elucidate the associated underlying mechanisms. ECa109 cells were cultured and treated with increasing concentrations of EGCG for various durations. Cell viability was evaluated using the MTT assay and apoptosis was detected using flow cytometry. The methylation status of the cyclin‑dependent kinase inhibitor 2A (p16) gene was analyzed using the methylation‑specific polymerase chain reaction (PCR). p16 mRNA and protein expression was measured using reverse transcription‑quantitative PCR and western blot analysis, respectively. The results of the present study demonstrated that, following treatment with EGCG, ECa109 cell viability was significantly decreased, while the rate of apoptosis was significantly increased (P<0.01), in a dose‑ and time‑dependent manner. Following treatment of ECa109 cells with EGCG, p16 gene demethylation, and its mRNA and protein expression, were significantly increased compared with the untreated cells (P<0.01). EGCG may induce ECa109 cell apoptosis and inhibit cell growth through p16 gene demethylation, which restores its expression.
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1 

Gao QY and Fang JY: Early esophageal cancer screening in China. Best Pract Res Clin Gastroenterol. 29:885–893. 2015. View Article : Google Scholar : PubMed/NCBI

2 

Aichler M, Motschmann M, Jütting U, Luber B, Becker K, Ott K, Lordick F, Langer R, Feith M, Siewert JR and Walch A: Epidermal growth factor receptor (EGFR) is an independent adverse prognostic factor in esophageal adenocarcinoma patients treated with cisplatin-based neoadjuvant chemotherapy. Oncotarget. 5:6620–6632. 2014. View Article : Google Scholar : PubMed/NCBI

3 

Podolski-Renić A, Andelković T, Banković J, Tanić N, Ruždijić S and Pešić M: The role of paclitaxel in the development and treatment of multidrug resistant cancer cell lines. Biomed Pharmacother. 65:345–353. 2011. View Article : Google Scholar : PubMed/NCBI

4 

Ding XW, Wu JH and Jiang CP: ABCG2: A potential marker of stem cells and novel target in stem cell and cancer therapy. Life Sci. 86:631–637. 2010. View Article : Google Scholar : PubMed/NCBI

5 

He X, Gao B, Zhou L and Xiong S: Green tea polyphenol epigallocatechin-3-gallate alleviated coxsackievirus B3-induced myocarditis through inhibiting viral replication, but not through inhibiting inflammatory responses. J Cardiovasc Pharmacol. 69:41–47. 2017. View Article : Google Scholar : PubMed/NCBI

6 

Yang CS and Landau JM: Effects of tea consumption on nutrition and health. J Nutr. 130:2409–2412. 2000.PubMed/NCBI

7 

Suganuma M, Takahashi A, Watanabe T, Iida K, Matsuzaki T, Yoshikawa HY and Fujiki H: Biophysical approach to mechanisms of cancer prevention and treatment with green tea catechins. Molecules. 21:pii: E15662016. View Article : Google Scholar

8 

Balentine DA, Wiseman SA and Bouwens LC: The chemistry of tea flavonoids. Crit Rev Food Sci Nutr. 37:693–704. 1997. View Article : Google Scholar : PubMed/NCBI

9 

Kerksick CM, Roberts MD, Dalbo VJ, Kreider RB and Willoughby DS: Changes in skeletal muscle proteolytic gene expression after prophylactic supplementation of EGCG and NAC and eccentric damage. Food Chem Toxicol. 61:47–52. 2013. View Article : Google Scholar : PubMed/NCBI

10 

Shimizu M, Adachi S, Masuda M, Kozawa O and Moriwaki H: Cancer chemoprevention with green tea catechins by targeting receptor tyrosine kinases. Mol Nutr Food Res. 55:832–843. 2011. View Article : Google Scholar : PubMed/NCBI

11 

Manohar M, Fatima I, Saxena R, Chandra V, Sankhwar PL and Dwivedi A: (−)-Epigallocatechin-3-gallate induces apoptosis in human endometrial adenocarcinoma cells via ROS generation and p38 MAP kinase activation. J Nutr Biochem. 24:940–947. 2013. View Article : Google Scholar : PubMed/NCBI

12 

Kuhn DJ, Burns AC, Kazi A and Dou QP: Direct inhibition of the ubiquitin-proteasome pathway by ester bond-containing green tea polyphenols is associated with increased expression of sterol regulatory element-binding protein 2 and LDL receptor. Biochim Biophys Acta. 1682:1–10. 2004. View Article : Google Scholar : PubMed/NCBI

13 

Li Y, Yuan YY, Meeran SM and Tollefsbol TO: Synergistic epigenetic reactivation of estrogen receptor-α (ERα) by combined green tea polyphenol and histone deacetylase inhibitor in ERα-negative breast cancer cells. Mol Cancer. 9:2742010. View Article : Google Scholar : PubMed/NCBI

14 

Mir MR, Shabir N, Wani KA, Shaff S, Hussain I, Banday MA, Chikan NA, Bilal S and Aejaz S: Association between p16, hMLH1 and E-cadherin promoter hypermethylation and intake of local hot salted tea and sun-dried foods in Kashmiris with gastric tumors. Asian Pac J Cancer Prev. 13:181–186. 2012. View Article : Google Scholar : PubMed/NCBI

15 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(−Delta Delta C(T)) Method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

16 

Wang Q, Wang Y, Ji Z, Chen X, Pan Y, Gao G, Gu H, Yang Y, Choi BC and Yan Y: Risk factors for multiple myeloma: A hospital-based case-control study in Northwest China. Cancer Epidemiol. 36:439–444. 2012. View Article : Google Scholar : PubMed/NCBI

17 

Wang J, Zhang W, Sun L, Yu H, Ni QX, Risch HA and Gao YT: Green tea drinking and risk of pancreatic cancer: A large-scale, population-based case-control study in urban Shanghai. Cancer Epidemiol. 36:e354–e358. 2012. View Article : Google Scholar : PubMed/NCBI

18 

Wu H, Xin Y, Xiao Y and Zhao J: Low-dose docetaxel combined with (−)-epigallocatechin-3-gallate inhibits angiogenesis and tumor growth in nude mice with gastric cancer xenografts. Cancer Biother Radiopharm. 27:204–209. 2012. View Article : Google Scholar : PubMed/NCBI

19 

Stearns ME, Amatangelo MD, Varma D, Varma D, Sell C and Goodyear SM: Combination therapy with epigallocatechin-3-gallate and doxorubicin in human prostate tumor modeling studies: Inhibition of metastatic tumor growth in severe combined immunodeficiency mice. Am J Pathol. 177:3169–3179. 2010. View Article : Google Scholar : PubMed/NCBI

20 

Shankar S, Marsh L and Srivastava RK: EGCG inhibits growth of human pancreatic tumors orthotopically implanted in Balb C nude mice through modulation of FKHRL1/FOXO3a and neuropilin. Mol Cell Biochem. 372:83–94. 2013. View Article : Google Scholar : PubMed/NCBI

21 

Kato K, Long NK, Makita H, Toida M, Yamashita T, Hatakeyama D, Hara A, Mori H and Shibata T: Effects of green tea polyphenol on methylation status of RECK gene and cancer cell invasion in oral squamous cell carcinoma cells. Br J Cancer. 99:647–654. 2008. View Article : Google Scholar : PubMed/NCBI

22 

Gao Z, Xu Z, Hung MS, Lin YC, Wang T, Gong M, Zhi X, Jablon DM and You L: Promoter demethylation of WIF-1 by epigallocatechin-3-gallate in lung cancer cells. Anticancer Res. 29:2025–2030. 2009.PubMed/NCBI

23 

Lima SC, Hernández-Vargas H, Simão T, Durand G, Kruel CD, Le Calvez-Kelm F, Pinto LF Ribeiro and Herceg Z: Identification of a DNA methylome signature of esophageal squamous cell carcinoma and potential epigenetic biomarkers. Epigenetics. 6:1217–1227. 2011. View Article : Google Scholar : PubMed/NCBI

24 

Rocco JW and Sidransky D: p16 (MTS-1/CDKN2/INK4a) in cancer progression. Exp Cell Res. 264:42–55. 2001. View Article : Google Scholar : PubMed/NCBI

25 

Islam M Nazmul, Yadav S, Haque M Hakimul, Munaz A, Islam F, Al Hossain MS, Gopalan V, Lam AK, Nguyen NT and Shiddiky MJ: Optical biosensing strategies for DNA methylation analysis. Biosens Bioelectron. 92:668–678. 2017. View Article : Google Scholar : PubMed/NCBI

26 

Fang M, Chen D and Yang CS: Dietary polyphenols may affect DNA methylation. J Nutr. 137 1 Suppl:223S–228S. 2007.PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Meng J, Tong Q, Liu X, Yu Z, Zhang J and Gao B: Epigallocatechin-3-gallate inhibits growth and induces apoptosis in esophageal cancer cells through the demethylation and reactivation of the p16 gene. Oncol Lett 14: 1152-1156, 2017.
APA
Meng, J., Tong, Q., Liu, X., Yu, Z., Zhang, J., & Gao, B. (2017). Epigallocatechin-3-gallate inhibits growth and induces apoptosis in esophageal cancer cells through the demethylation and reactivation of the p16 gene. Oncology Letters, 14, 1152-1156. https://doi.org/10.3892/ol.2017.6248
MLA
Meng, J., Tong, Q., Liu, X., Yu, Z., Zhang, J., Gao, B."Epigallocatechin-3-gallate inhibits growth and induces apoptosis in esophageal cancer cells through the demethylation and reactivation of the p16 gene". Oncology Letters 14.1 (2017): 1152-1156.
Chicago
Meng, J., Tong, Q., Liu, X., Yu, Z., Zhang, J., Gao, B."Epigallocatechin-3-gallate inhibits growth and induces apoptosis in esophageal cancer cells through the demethylation and reactivation of the p16 gene". Oncology Letters 14, no. 1 (2017): 1152-1156. https://doi.org/10.3892/ol.2017.6248
Copy and paste a formatted citation
x
Spandidos Publications style
Meng J, Tong Q, Liu X, Yu Z, Zhang J and Gao B: Epigallocatechin-3-gallate inhibits growth and induces apoptosis in esophageal cancer cells through the demethylation and reactivation of the p16 gene. Oncol Lett 14: 1152-1156, 2017.
APA
Meng, J., Tong, Q., Liu, X., Yu, Z., Zhang, J., & Gao, B. (2017). Epigallocatechin-3-gallate inhibits growth and induces apoptosis in esophageal cancer cells through the demethylation and reactivation of the p16 gene. Oncology Letters, 14, 1152-1156. https://doi.org/10.3892/ol.2017.6248
MLA
Meng, J., Tong, Q., Liu, X., Yu, Z., Zhang, J., Gao, B."Epigallocatechin-3-gallate inhibits growth and induces apoptosis in esophageal cancer cells through the demethylation and reactivation of the p16 gene". Oncology Letters 14.1 (2017): 1152-1156.
Chicago
Meng, J., Tong, Q., Liu, X., Yu, Z., Zhang, J., Gao, B."Epigallocatechin-3-gallate inhibits growth and induces apoptosis in esophageal cancer cells through the demethylation and reactivation of the p16 gene". Oncology Letters 14, no. 1 (2017): 1152-1156. https://doi.org/10.3892/ol.2017.6248
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